Interactions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA
Abstract The flavescence dorée phytoplasma undergoes a propagative cycle in its insect vectors by first interacting with the insect cell surfaces, primarily in the midgut lumen and subsequently in the salivary glands. Adhesion of flavescence dorée phytoplasma to insect cells is mediated by the adhes...
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| Format: | Article |
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Nature Portfolio
2021-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-90809-z |
| _version_ | 1818405338100006912 |
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| author | Nathalie Arricau-Bouvery Sybille Duret Marie-Pierre Dubrana Delphine Desqué Sandrine Eveillard Lysiane Brocard Sylvie Malembic-Maher Xavier Foissac |
| author_facet | Nathalie Arricau-Bouvery Sybille Duret Marie-Pierre Dubrana Delphine Desqué Sandrine Eveillard Lysiane Brocard Sylvie Malembic-Maher Xavier Foissac |
| author_sort | Nathalie Arricau-Bouvery |
| collection | DOAJ |
| description | Abstract The flavescence dorée phytoplasma undergoes a propagative cycle in its insect vectors by first interacting with the insect cell surfaces, primarily in the midgut lumen and subsequently in the salivary glands. Adhesion of flavescence dorée phytoplasma to insect cells is mediated by the adhesin VmpA. We hypothesize that VmpA may have lectin-like activity, similar to several adhesins of bacteria that invade the insect gut. We first demonstrated that the luminal surface of the midgut and the basal surface of the salivary gland cells of the natural vector Scaphoideus titanus and those of the experimental vector Euscelidius variegatus were differentially glycosylated. Using ELISA, inhibition and competitive adhesion assays, and protein overlay assays in the Euva-6 insect cell line, we showed that the protein VmpA binds insect proteins in a lectin-like manner. In conclusion, the results of this study indicate that N-acetylglucosamine and mannose present on the surfaces of the midgut and salivary glands serve as recognition sites for the phytoplasma adhesin VmpA. |
| first_indexed | 2024-12-14T08:54:27Z |
| format | Article |
| id | doaj.art-9d9118444b3f4bd68d7ed80c6a87ed8e |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T08:54:27Z |
| publishDate | 2021-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9d9118444b3f4bd68d7ed80c6a87ed8e2022-12-21T23:08:58ZengNature PortfolioScientific Reports2045-23222021-05-0111111410.1038/s41598-021-90809-zInteractions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpANathalie Arricau-Bouvery0Sybille Duret1Marie-Pierre Dubrana2Delphine Desqué3Sandrine Eveillard4Lysiane Brocard5Sylvie Malembic-Maher6Xavier Foissac7Univ. Bordeaux, INRAE, Biologie du Fruit et Pathologie, UMR 1332Univ. Bordeaux, INRAE, Biologie du Fruit et Pathologie, UMR 1332Univ. Bordeaux, INRAE, Biologie du Fruit et Pathologie, UMR 1332Univ. Bordeaux, INRAE, Biologie du Fruit et Pathologie, UMR 1332Univ. Bordeaux, INRAE, Biologie du Fruit et Pathologie, UMR 1332Univ. Bordeaux, CNRS, INSERM, Bordeaux Imaging Center, BIC, UMS 3420, US 4Univ. Bordeaux, INRAE, Biologie du Fruit et Pathologie, UMR 1332Univ. Bordeaux, INRAE, Biologie du Fruit et Pathologie, UMR 1332Abstract The flavescence dorée phytoplasma undergoes a propagative cycle in its insect vectors by first interacting with the insect cell surfaces, primarily in the midgut lumen and subsequently in the salivary glands. Adhesion of flavescence dorée phytoplasma to insect cells is mediated by the adhesin VmpA. We hypothesize that VmpA may have lectin-like activity, similar to several adhesins of bacteria that invade the insect gut. We first demonstrated that the luminal surface of the midgut and the basal surface of the salivary gland cells of the natural vector Scaphoideus titanus and those of the experimental vector Euscelidius variegatus were differentially glycosylated. Using ELISA, inhibition and competitive adhesion assays, and protein overlay assays in the Euva-6 insect cell line, we showed that the protein VmpA binds insect proteins in a lectin-like manner. In conclusion, the results of this study indicate that N-acetylglucosamine and mannose present on the surfaces of the midgut and salivary glands serve as recognition sites for the phytoplasma adhesin VmpA.https://doi.org/10.1038/s41598-021-90809-z |
| spellingShingle | Nathalie Arricau-Bouvery Sybille Duret Marie-Pierre Dubrana Delphine Desqué Sandrine Eveillard Lysiane Brocard Sylvie Malembic-Maher Xavier Foissac Interactions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA Scientific Reports |
| title | Interactions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA |
| title_full | Interactions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA |
| title_fullStr | Interactions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA |
| title_full_unstemmed | Interactions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA |
| title_short | Interactions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA |
| title_sort | interactions between the flavescence doree phytoplasma and its insect vector indicate lectin type adhesion mediated by the adhesin vmpa |
| url | https://doi.org/10.1038/s41598-021-90809-z |
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