Protein Elicitor PemG1 from Magnaporthe grisea Induces Systemic Acquired Resistance (SAR) in Plants
Elicitors can stimulate defense responses in plants and have become a popular strategy in plant disease control. Previously, we isolated a novel protein elicitor, PemG1, from Magnaporthe grisea. In the present study, PemG1 protein expressed in and purified from Escherichia coli improved resistance o...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2011-10-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-01-11-0003 |
| _version_ | 1818914953852092416 |
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| author | Dong-Hai Peng De-Wen Qiu Li-Fang Ruan Chen-Fei Zhou Ming Sun |
| author_facet | Dong-Hai Peng De-Wen Qiu Li-Fang Ruan Chen-Fei Zhou Ming Sun |
| author_sort | Dong-Hai Peng |
| collection | DOAJ |
| description | Elicitors can stimulate defense responses in plants and have become a popular strategy in plant disease control. Previously, we isolated a novel protein elicitor, PemG1, from Magnaporthe grisea. In the present study, PemG1 protein expressed in and purified from Escherichia coli improved resistance of rice and Arabidopsis to bacterial infection, induced transient expression of pathogenesis-related (PR) genes, and increased accumulation of hydrogen peroxide in rice. The effects of PemG1 on disease resistance and PR gene expression were mobilized systemically throughout the rice plant and persisted for more than 28 days. PemG1-induced accumulation of OsPR-1a in rice was prevented by the calcium channel blockers LaCl3, BAPTA, EGTA, W7, and TFP. Arabidopsis mutants that are insensitive to jasmonic acid (JA) and ethylene showed increased resistance to bacterial infection after PemG1 treatment but PemG1 did not affect resistance of mutants with an impaired salicylic acid (SA) transduction pathway. In rice, PemG1 induced overexpressions of the SA signal-related genes (OsEDS1, OsPAL1, and OsNH1) but not the JA pathway-related genes (OsLOX2 and OsAOS2). Our findings reveal that PemG1 protein can function as an activator of plant disease resistance, and the PemG1-mediated systemic acquired resistance is modulated by SA- and Ca2+-related signaling pathways. |
| first_indexed | 2024-12-19T23:54:35Z |
| format | Article |
| id | doaj.art-9d9118e35c5c4df783ab741394f6d984 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-19T23:54:35Z |
| publishDate | 2011-10-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-9d9118e35c5c4df783ab741394f6d9842022-12-21T20:01:03ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062011-10-0124101239124610.1094/MPMI-01-11-0003Protein Elicitor PemG1 from Magnaporthe grisea Induces Systemic Acquired Resistance (SAR) in PlantsDong-Hai PengDe-Wen QiuLi-Fang RuanChen-Fei ZhouMing SunElicitors can stimulate defense responses in plants and have become a popular strategy in plant disease control. Previously, we isolated a novel protein elicitor, PemG1, from Magnaporthe grisea. In the present study, PemG1 protein expressed in and purified from Escherichia coli improved resistance of rice and Arabidopsis to bacterial infection, induced transient expression of pathogenesis-related (PR) genes, and increased accumulation of hydrogen peroxide in rice. The effects of PemG1 on disease resistance and PR gene expression were mobilized systemically throughout the rice plant and persisted for more than 28 days. PemG1-induced accumulation of OsPR-1a in rice was prevented by the calcium channel blockers LaCl3, BAPTA, EGTA, W7, and TFP. Arabidopsis mutants that are insensitive to jasmonic acid (JA) and ethylene showed increased resistance to bacterial infection after PemG1 treatment but PemG1 did not affect resistance of mutants with an impaired salicylic acid (SA) transduction pathway. In rice, PemG1 induced overexpressions of the SA signal-related genes (OsEDS1, OsPAL1, and OsNH1) but not the JA pathway-related genes (OsLOX2 and OsAOS2). Our findings reveal that PemG1 protein can function as an activator of plant disease resistance, and the PemG1-mediated systemic acquired resistance is modulated by SA- and Ca2+-related signaling pathways.https://apsjournals.apsnet.org/doi/10.1094/MPMI-01-11-0003 |
| spellingShingle | Dong-Hai Peng De-Wen Qiu Li-Fang Ruan Chen-Fei Zhou Ming Sun Protein Elicitor PemG1 from Magnaporthe grisea Induces Systemic Acquired Resistance (SAR) in Plants Molecular Plant-Microbe Interactions |
| title | Protein Elicitor PemG1 from Magnaporthe grisea Induces Systemic Acquired Resistance (SAR) in Plants |
| title_full | Protein Elicitor PemG1 from Magnaporthe grisea Induces Systemic Acquired Resistance (SAR) in Plants |
| title_fullStr | Protein Elicitor PemG1 from Magnaporthe grisea Induces Systemic Acquired Resistance (SAR) in Plants |
| title_full_unstemmed | Protein Elicitor PemG1 from Magnaporthe grisea Induces Systemic Acquired Resistance (SAR) in Plants |
| title_short | Protein Elicitor PemG1 from Magnaporthe grisea Induces Systemic Acquired Resistance (SAR) in Plants |
| title_sort | protein elicitor pemg1 from magnaporthe grisea induces systemic acquired resistance sar in plants |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-01-11-0003 |
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