<i>Indigo Pulverata Levis</i> (Chung-Dae, <i>Persicaria tinctoria</i>) Alleviates Atopic Dermatitis-like Inflammatory Responses In Vivo and In Vitro

<i>Indigo Pulverata Levis</i> (Chung-Dae, <i>Persicaria tinctoria</i>) Alleviates Atopic Dermatitis-like Inflammatory Responses In Vivo and In Vitro

Atopic dermatitis (AD) is a chronic inflammatory skin disease associated with a type 2 T helper cell (Th2) immune response. The <i>Indigo</i><i>Pulverata Levis</i> extract (CHD) is used in traditional Southeast Asian medicine; however, its beneficial effects on AD remain unin...

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Bibliographic Details
Main Authors: Ga-Yul Min, Ji-Hye Kim, Tae-In Kim, Won-Kyung Cho, Ju-Hye Yang, Jin-Yeul Ma
Format: Article
Language:English
Published: MDPI AG 2022-01-01
Series:International Journal of Molecular Sciences
Subjects:
atopic dermatitis
<i>Indigo Pulverata Levis</i>
immune-cell infiltration
skin thickness
NF-κB p65
proinflammatory cytokines
Online Access:https://www.mdpi.com/1422-0067/23/1/553
Description
Summary:Atopic dermatitis (AD) is a chronic inflammatory skin disease associated with a type 2 T helper cell (Th2) immune response. The <i>Indigo</i><i>Pulverata Levis</i> extract (CHD) is used in traditional Southeast Asian medicine; however, its beneficial effects on AD remain uninvestigated. Therefore, we investigated the therapeutic effects of CHD in 2,4-dinitrochlorobenzene (DNCB)-induced BALB/c mice and tumor necrosis factor (TNF)-α- and interferon gamma (IFN)-γ-stimulated HaCaT cells. We evaluated immune cell infiltration, skin thickness, and the serum IgE and TNF-α levels in DNCB-induced AD mice. Moreover, we measured the expression levels of pro-inflammatory cytokines, mitogen-activated protein kinase (MAPK), and the nuclear factor-kappa B (NF-κB) in the mice dorsal skin. We also studied the effect of CHD on the translocation of NF-κB p65 and inflammatory chemokines in HaCaT cells. Our in vivo results revealed that CHD reduced the dermis and epidermis thicknesses and inhibited immune cell infiltration. Furthermore, it suppressed the proinflammatory cytokine expression and MAPK and NF-κB phosphorylations in the skin tissue and decreased serum IgE and TNF-α levels. In vitro results indicated that CHD downregulated inflammatory chemokines and blocked NF-κB p65 translocation. Thus, we deduced that CHD is a potential drug candidate for AD treatment.
ISSN:1661-6596
1422-0067

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