<i>Indigo Pulverata Levis</i> (Chung-Dae, <i>Persicaria tinctoria</i>) Alleviates Atopic Dermatitis-like Inflammatory Responses In Vivo and In Vitro
Atopic dermatitis (AD) is a chronic inflammatory skin disease associated with a type 2 T helper cell (Th2) immune response. The <i>Indigo</i><i>Pulverata Levis</i> extract (CHD) is used in traditional Southeast Asian medicine; however, its beneficial effects on AD remain unin...
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2022-01-01
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author | Ga-Yul Min Ji-Hye Kim Tae-In Kim Won-Kyung Cho Ju-Hye Yang Jin-Yeul Ma |
author_facet | Ga-Yul Min Ji-Hye Kim Tae-In Kim Won-Kyung Cho Ju-Hye Yang Jin-Yeul Ma |
author_sort | Ga-Yul Min |
collection | DOAJ |
description | Atopic dermatitis (AD) is a chronic inflammatory skin disease associated with a type 2 T helper cell (Th2) immune response. The <i>Indigo</i><i>Pulverata Levis</i> extract (CHD) is used in traditional Southeast Asian medicine; however, its beneficial effects on AD remain uninvestigated. Therefore, we investigated the therapeutic effects of CHD in 2,4-dinitrochlorobenzene (DNCB)-induced BALB/c mice and tumor necrosis factor (TNF)-α- and interferon gamma (IFN)-γ-stimulated HaCaT cells. We evaluated immune cell infiltration, skin thickness, and the serum IgE and TNF-α levels in DNCB-induced AD mice. Moreover, we measured the expression levels of pro-inflammatory cytokines, mitogen-activated protein kinase (MAPK), and the nuclear factor-kappa B (NF-κB) in the mice dorsal skin. We also studied the effect of CHD on the translocation of NF-κB p65 and inflammatory chemokines in HaCaT cells. Our in vivo results revealed that CHD reduced the dermis and epidermis thicknesses and inhibited immune cell infiltration. Furthermore, it suppressed the proinflammatory cytokine expression and MAPK and NF-κB phosphorylations in the skin tissue and decreased serum IgE and TNF-α levels. In vitro results indicated that CHD downregulated inflammatory chemokines and blocked NF-κB p65 translocation. Thus, we deduced that CHD is a potential drug candidate for AD treatment. |
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series | International Journal of Molecular Sciences |
spelling | doaj.art-9d92f029b3644bf29b7bfbcecd88bad32023-11-23T11:41:47ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-01-0123155310.3390/ijms23010553<i>Indigo Pulverata Levis</i> (Chung-Dae, <i>Persicaria tinctoria</i>) Alleviates Atopic Dermatitis-like Inflammatory Responses In Vivo and In VitroGa-Yul Min0Ji-Hye Kim1Tae-In Kim2Won-Kyung Cho3Ju-Hye Yang4Jin-Yeul Ma5Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, 70 Cheomdan-ro, Dong-gu, Daegu 41062, KoreaKorean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, 70 Cheomdan-ro, Dong-gu, Daegu 41062, KoreaKorean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, 70 Cheomdan-ro, Dong-gu, Daegu 41062, KoreaKorean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, 70 Cheomdan-ro, Dong-gu, Daegu 41062, KoreaKorean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, 70 Cheomdan-ro, Dong-gu, Daegu 41062, KoreaKorean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, 70 Cheomdan-ro, Dong-gu, Daegu 41062, KoreaAtopic dermatitis (AD) is a chronic inflammatory skin disease associated with a type 2 T helper cell (Th2) immune response. The <i>Indigo</i><i>Pulverata Levis</i> extract (CHD) is used in traditional Southeast Asian medicine; however, its beneficial effects on AD remain uninvestigated. Therefore, we investigated the therapeutic effects of CHD in 2,4-dinitrochlorobenzene (DNCB)-induced BALB/c mice and tumor necrosis factor (TNF)-α- and interferon gamma (IFN)-γ-stimulated HaCaT cells. We evaluated immune cell infiltration, skin thickness, and the serum IgE and TNF-α levels in DNCB-induced AD mice. Moreover, we measured the expression levels of pro-inflammatory cytokines, mitogen-activated protein kinase (MAPK), and the nuclear factor-kappa B (NF-κB) in the mice dorsal skin. We also studied the effect of CHD on the translocation of NF-κB p65 and inflammatory chemokines in HaCaT cells. Our in vivo results revealed that CHD reduced the dermis and epidermis thicknesses and inhibited immune cell infiltration. Furthermore, it suppressed the proinflammatory cytokine expression and MAPK and NF-κB phosphorylations in the skin tissue and decreased serum IgE and TNF-α levels. In vitro results indicated that CHD downregulated inflammatory chemokines and blocked NF-κB p65 translocation. Thus, we deduced that CHD is a potential drug candidate for AD treatment.https://www.mdpi.com/1422-0067/23/1/553atopic dermatitis<i>Indigo Pulverata Levis</i>immune-cell infiltrationskin thicknessNF-κB p65proinflammatory cytokines |
spellingShingle | Ga-Yul Min Ji-Hye Kim Tae-In Kim Won-Kyung Cho Ju-Hye Yang Jin-Yeul Ma <i>Indigo Pulverata Levis</i> (Chung-Dae, <i>Persicaria tinctoria</i>) Alleviates Atopic Dermatitis-like Inflammatory Responses In Vivo and In Vitro International Journal of Molecular Sciences atopic dermatitis <i>Indigo Pulverata Levis</i> immune-cell infiltration skin thickness NF-κB p65 proinflammatory cytokines |
title | <i>Indigo Pulverata Levis</i> (Chung-Dae, <i>Persicaria tinctoria</i>) Alleviates Atopic Dermatitis-like Inflammatory Responses In Vivo and In Vitro |
title_full | <i>Indigo Pulverata Levis</i> (Chung-Dae, <i>Persicaria tinctoria</i>) Alleviates Atopic Dermatitis-like Inflammatory Responses In Vivo and In Vitro |
title_fullStr | <i>Indigo Pulverata Levis</i> (Chung-Dae, <i>Persicaria tinctoria</i>) Alleviates Atopic Dermatitis-like Inflammatory Responses In Vivo and In Vitro |
title_full_unstemmed | <i>Indigo Pulverata Levis</i> (Chung-Dae, <i>Persicaria tinctoria</i>) Alleviates Atopic Dermatitis-like Inflammatory Responses In Vivo and In Vitro |
title_short | <i>Indigo Pulverata Levis</i> (Chung-Dae, <i>Persicaria tinctoria</i>) Alleviates Atopic Dermatitis-like Inflammatory Responses In Vivo and In Vitro |
title_sort | i indigo pulverata levis i chung dae i persicaria tinctoria i alleviates atopic dermatitis like inflammatory responses in vivo and in vitro |
topic | atopic dermatitis <i>Indigo Pulverata Levis</i> immune-cell infiltration skin thickness NF-κB p65 proinflammatory cytokines |
url | https://www.mdpi.com/1422-0067/23/1/553 |
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