Exploring the Transcriptome Dynamics of In Vivo <i>Theileria annulata</i> Infection in Crossbred Cattle
The molecular changes occurring in the host in response to in vivo <i>Theileria annulata</i> parasitic infection are not well understood. Therefore, the present study investigated the differential expression profiles of peripheral blood mononuclear cells (PBMCs) across <i>Theileria...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/9/1663 |
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| author | Sonika Ahlawat Vikas Choudhary Reena Arora Ashish Kumar Mandeep Kaur Pooja Chhabra |
| author_facet | Sonika Ahlawat Vikas Choudhary Reena Arora Ashish Kumar Mandeep Kaur Pooja Chhabra |
| author_sort | Sonika Ahlawat |
| collection | DOAJ |
| description | The molecular changes occurring in the host in response to in vivo <i>Theileria annulata</i> parasitic infection are not well understood. Therefore, the present study investigated the differential expression profiles of peripheral blood mononuclear cells (PBMCs) across <i>Theileria annulata</i>-infected and non-infected crossbred cows. The differential expression profiles from PBMCs of infected and non-infected crossbred cows were generated by RNA sequencing. A marked difference in the expression of genes associated with innate immunity (<i>FTH1</i>, <i>ACTB</i>, <i>ISG15</i>) was observed between the two groups. The over-represented pathways in <i>Theileria annulata</i>-infected cows were associated with the immune system and regulation of the mitotic cycle. Enriched genes and pathways in non-infected animals were associated with the maintenance of chromatin integrity and cell structure. The highly connected genes identified in this study form potential candidates for further investigation into host–parasite interactions in cattle. An improved understanding of the transcriptomic dynamics during theileriosis would lead to underpinning molecular level differences related to the health status of cattle. |
| first_indexed | 2024-03-10T22:43:36Z |
| format | Article |
| id | doaj.art-9d94baa0d0d74b169ba670e6b7802feb |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T22:43:36Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-9d94baa0d0d74b169ba670e6b7802feb2023-11-19T10:51:57ZengMDPI AGGenes2073-44252023-08-01149166310.3390/genes14091663Exploring the Transcriptome Dynamics of In Vivo <i>Theileria annulata</i> Infection in Crossbred CattleSonika Ahlawat0Vikas Choudhary1Reena Arora2Ashish Kumar3Mandeep Kaur4Pooja Chhabra5ICAR-National Bureau of Animal Genetic Resources, Karnal 132001, Haryana, IndiaDepartment of Animal Husbandry and Dairying, Karnal 132001, Haryana, IndiaICAR-National Bureau of Animal Genetic Resources, Karnal 132001, Haryana, IndiaICAR-National Bureau of Animal Genetic Resources, Karnal 132001, Haryana, IndiaICAR-National Bureau of Animal Genetic Resources, Karnal 132001, Haryana, IndiaICAR-National Bureau of Animal Genetic Resources, Karnal 132001, Haryana, IndiaThe molecular changes occurring in the host in response to in vivo <i>Theileria annulata</i> parasitic infection are not well understood. Therefore, the present study investigated the differential expression profiles of peripheral blood mononuclear cells (PBMCs) across <i>Theileria annulata</i>-infected and non-infected crossbred cows. The differential expression profiles from PBMCs of infected and non-infected crossbred cows were generated by RNA sequencing. A marked difference in the expression of genes associated with innate immunity (<i>FTH1</i>, <i>ACTB</i>, <i>ISG15</i>) was observed between the two groups. The over-represented pathways in <i>Theileria annulata</i>-infected cows were associated with the immune system and regulation of the mitotic cycle. Enriched genes and pathways in non-infected animals were associated with the maintenance of chromatin integrity and cell structure. The highly connected genes identified in this study form potential candidates for further investigation into host–parasite interactions in cattle. An improved understanding of the transcriptomic dynamics during theileriosis would lead to underpinning molecular level differences related to the health status of cattle.https://www.mdpi.com/2073-4425/14/9/1663differential expressionmRNAparasiteRNAseqtheileriosis |
| spellingShingle | Sonika Ahlawat Vikas Choudhary Reena Arora Ashish Kumar Mandeep Kaur Pooja Chhabra Exploring the Transcriptome Dynamics of In Vivo <i>Theileria annulata</i> Infection in Crossbred Cattle Genes differential expression mRNA parasite RNAseq theileriosis |
| title | Exploring the Transcriptome Dynamics of In Vivo <i>Theileria annulata</i> Infection in Crossbred Cattle |
| title_full | Exploring the Transcriptome Dynamics of In Vivo <i>Theileria annulata</i> Infection in Crossbred Cattle |
| title_fullStr | Exploring the Transcriptome Dynamics of In Vivo <i>Theileria annulata</i> Infection in Crossbred Cattle |
| title_full_unstemmed | Exploring the Transcriptome Dynamics of In Vivo <i>Theileria annulata</i> Infection in Crossbred Cattle |
| title_short | Exploring the Transcriptome Dynamics of In Vivo <i>Theileria annulata</i> Infection in Crossbred Cattle |
| title_sort | exploring the transcriptome dynamics of in vivo i theileria annulata i infection in crossbred cattle |
| topic | differential expression mRNA parasite RNAseq theileriosis |
| url | https://www.mdpi.com/2073-4425/14/9/1663 |
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