Evaluating the antimicrobial, apoptotic, and cancer cell gene delivery properties of protein-capped gold nanoparticles synthesized from the edible mycorrhizal fungus Tricholoma crassum
Abstract Biosynthesis of gold nanoparticles of distinct geometric shapes with highly functional protein coats without additional capping steps is rarely reported. This study describes green synthesis of protein-coated gold nanoparticles for the first time from the edible, mycorrhizal fungus Tricholo...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-05-01
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| Series: | Nanoscale Research Letters |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s11671-018-2561-y |
| _version_ | 1797761189781962752 |
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| author | Arpita Basu Sarmishtha Ray Supriyo Chowdhury Arnab Sarkar Deba Prasad Mandal Shamee Bhattacharjee Surekha Kundu |
| author_facet | Arpita Basu Sarmishtha Ray Supriyo Chowdhury Arnab Sarkar Deba Prasad Mandal Shamee Bhattacharjee Surekha Kundu |
| author_sort | Arpita Basu |
| collection | DOAJ |
| description | Abstract Biosynthesis of gold nanoparticles of distinct geometric shapes with highly functional protein coats without additional capping steps is rarely reported. This study describes green synthesis of protein-coated gold nanoparticles for the first time from the edible, mycorrhizal fungus Tricholoma crassum (Berk.) Sacc. The nanoparticles were of the size range 5–25 nm and of different shapes. Spectroscopic analysis showed red shift of the absorption maxima with longer reaction period during production and blue shift with increase in pH. These were characterized with spectroscopy, SEM, TEM, AFM, XRD, and DLS. The particle size could be altered by changing synthesis parameters. These had potent antimicrobial activity against bacteria, fungi, and multi-drug-resistant pathogenic bacteria. These also had inhibitory effect on the growth kinetics of bacteria and germination of fungal spores. These showed apoptotic properties on eukaryotic cells when tested with comet assays. Moreover, the particles are capped with a natural 40 kDa protein which was utilized as attachment sites for genes to be delivered into sarcoma cancer cells. The present work also attempted at optimizing safe dosage of these nanoparticles using hemolysis assays, for application in therapy. Large-scale production of the nanoparticles in fermentors and other possible applications of the particles have been discussed. |
| first_indexed | 2024-03-12T19:09:20Z |
| format | Article |
| id | doaj.art-9d96feaa43e5423bbd9e557900be8d85 |
| institution | Directory Open Access Journal |
| issn | 1931-7573 1556-276X |
| language | English |
| last_indexed | 2024-03-12T19:09:20Z |
| publishDate | 2018-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nanoscale Research Letters |
| spelling | doaj.art-9d96feaa43e5423bbd9e557900be8d852023-08-02T06:00:29ZengSpringerOpenNanoscale Research Letters1931-75731556-276X2018-05-0113111610.1186/s11671-018-2561-yEvaluating the antimicrobial, apoptotic, and cancer cell gene delivery properties of protein-capped gold nanoparticles synthesized from the edible mycorrhizal fungus Tricholoma crassumArpita Basu0Sarmishtha Ray1Supriyo Chowdhury2Arnab Sarkar3Deba Prasad Mandal4Shamee Bhattacharjee5Surekha Kundu6Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of CalcuttaMolecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of CalcuttaMolecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of CalcuttaDepartment of Zoology, West Bengal State UniversityDepartment of Zoology, West Bengal State UniversityDepartment of Zoology, West Bengal State UniversityMolecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of CalcuttaAbstract Biosynthesis of gold nanoparticles of distinct geometric shapes with highly functional protein coats without additional capping steps is rarely reported. This study describes green synthesis of protein-coated gold nanoparticles for the first time from the edible, mycorrhizal fungus Tricholoma crassum (Berk.) Sacc. The nanoparticles were of the size range 5–25 nm and of different shapes. Spectroscopic analysis showed red shift of the absorption maxima with longer reaction period during production and blue shift with increase in pH. These were characterized with spectroscopy, SEM, TEM, AFM, XRD, and DLS. The particle size could be altered by changing synthesis parameters. These had potent antimicrobial activity against bacteria, fungi, and multi-drug-resistant pathogenic bacteria. These also had inhibitory effect on the growth kinetics of bacteria and germination of fungal spores. These showed apoptotic properties on eukaryotic cells when tested with comet assays. Moreover, the particles are capped with a natural 40 kDa protein which was utilized as attachment sites for genes to be delivered into sarcoma cancer cells. The present work also attempted at optimizing safe dosage of these nanoparticles using hemolysis assays, for application in therapy. Large-scale production of the nanoparticles in fermentors and other possible applications of the particles have been discussed.http://link.springer.com/article/10.1186/s11671-018-2561-yGreen synthesisProtein-capped gold nanoparticlesAntimicrobialApoptosisCancer cell gene delivery |
| spellingShingle | Arpita Basu Sarmishtha Ray Supriyo Chowdhury Arnab Sarkar Deba Prasad Mandal Shamee Bhattacharjee Surekha Kundu Evaluating the antimicrobial, apoptotic, and cancer cell gene delivery properties of protein-capped gold nanoparticles synthesized from the edible mycorrhizal fungus Tricholoma crassum Nanoscale Research Letters Green synthesis Protein-capped gold nanoparticles Antimicrobial Apoptosis Cancer cell gene delivery |
| title | Evaluating the antimicrobial, apoptotic, and cancer cell gene delivery properties of protein-capped gold nanoparticles synthesized from the edible mycorrhizal fungus Tricholoma crassum |
| title_full | Evaluating the antimicrobial, apoptotic, and cancer cell gene delivery properties of protein-capped gold nanoparticles synthesized from the edible mycorrhizal fungus Tricholoma crassum |
| title_fullStr | Evaluating the antimicrobial, apoptotic, and cancer cell gene delivery properties of protein-capped gold nanoparticles synthesized from the edible mycorrhizal fungus Tricholoma crassum |
| title_full_unstemmed | Evaluating the antimicrobial, apoptotic, and cancer cell gene delivery properties of protein-capped gold nanoparticles synthesized from the edible mycorrhizal fungus Tricholoma crassum |
| title_short | Evaluating the antimicrobial, apoptotic, and cancer cell gene delivery properties of protein-capped gold nanoparticles synthesized from the edible mycorrhizal fungus Tricholoma crassum |
| title_sort | evaluating the antimicrobial apoptotic and cancer cell gene delivery properties of protein capped gold nanoparticles synthesized from the edible mycorrhizal fungus tricholoma crassum |
| topic | Green synthesis Protein-capped gold nanoparticles Antimicrobial Apoptosis Cancer cell gene delivery |
| url | http://link.springer.com/article/10.1186/s11671-018-2561-y |
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