Toxoplasma gondii tachyzoite-extract acts as a potent immunomodulator against allergic sensitization and airway inflammation
Abstract Epidemiological and experimental studies have shown an inverse relationship between infections with certain parasites and a reduced incidence of allergic diseases. We and others have shown that infection with Toxoplasma gondii prevents the development of allergy in mice. To establish whethe...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-15663-4 |
| _version_ | 1818751444067549184 |
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| author | Mirjana Drinić Angelika Wagner Priya Sarate Christian Zwicker Elke Korb Gerhard Loupal Roman Peschke Anja Joachim Ursula Wiedermann Irma Schabussova |
| author_facet | Mirjana Drinić Angelika Wagner Priya Sarate Christian Zwicker Elke Korb Gerhard Loupal Roman Peschke Anja Joachim Ursula Wiedermann Irma Schabussova |
| author_sort | Mirjana Drinić |
| collection | DOAJ |
| description | Abstract Epidemiological and experimental studies have shown an inverse relationship between infections with certain parasites and a reduced incidence of allergic diseases. We and others have shown that infection with Toxoplasma gondii prevents the development of allergy in mice. To establish whether this beneficial effect could be recapitulated by soluble products of this parasite, we tested an extract derived from T. gondii tachyzoites. Immunization of BALB/c mice with tachyzoites lysate antigen (TLA) elicited mixed Th1/Th2 responses. When TLA was applied together with the sensitizing ovalbumin (OVA), the development of allergic airway inflammation was reduced, with decreased airway hyperresponsiveness associated with reduced peribronchial and perivascular cellular infiltration, reduced production of OVA-specific Th2 cytokines in lungs and spleens and reduced levels of serum OVA-specific IgG1 as well as IgE-dependent basophil degranulation. Of note, TLA retained its immunomodulatory properties, inducing high levels of IL-6, TNFα, IL-10 and IL-12p70 in bone marrow-derived dendritic cells after heat-inactivation or proteinase K-treatment for disruption of proteins, but not after sodium metaperiodate-treatment that degrades carbohydrate structures, suggesting that carbohydrates may play a role in immunomodulatory properties of TLA. Here we show that extracts derived from parasites may replicate the benefits of parasitic infection, offering new therapies for immune-mediated disorders. |
| first_indexed | 2024-12-18T04:35:40Z |
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| id | doaj.art-9d98377b0ea1474b8fe56b7f5c6479bc |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T04:35:40Z |
| publishDate | 2017-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9d98377b0ea1474b8fe56b7f5c6479bc2022-12-21T21:20:52ZengNature PortfolioScientific Reports2045-23222017-11-017111210.1038/s41598-017-15663-4Toxoplasma gondii tachyzoite-extract acts as a potent immunomodulator against allergic sensitization and airway inflammationMirjana Drinić0Angelika Wagner1Priya Sarate2Christian Zwicker3Elke Korb4Gerhard Loupal5Roman Peschke6Anja Joachim7Ursula Wiedermann8Irma Schabussova9Institute of Specific Prophylaxis and Tropical Medicine, Medical University of ViennaInstitute of Specific Prophylaxis and Tropical Medicine, Medical University of ViennaInstitute of Specific Prophylaxis and Tropical Medicine, Medical University of ViennaInstitute of Specific Prophylaxis and Tropical Medicine, Medical University of ViennaInstitute of Specific Prophylaxis and Tropical Medicine, Medical University of ViennaInstitute of Pathology and Forensic Veterinary Medicine, Department of Pathobiology, University of Veterinary Medicine ViennaInstitute of Parasitology, Department of Pathobiology, University of Veterinary Medicine ViennaInstitute of Parasitology, Department of Pathobiology, University of Veterinary Medicine ViennaInstitute of Specific Prophylaxis and Tropical Medicine, Medical University of ViennaInstitute of Specific Prophylaxis and Tropical Medicine, Medical University of ViennaAbstract Epidemiological and experimental studies have shown an inverse relationship between infections with certain parasites and a reduced incidence of allergic diseases. We and others have shown that infection with Toxoplasma gondii prevents the development of allergy in mice. To establish whether this beneficial effect could be recapitulated by soluble products of this parasite, we tested an extract derived from T. gondii tachyzoites. Immunization of BALB/c mice with tachyzoites lysate antigen (TLA) elicited mixed Th1/Th2 responses. When TLA was applied together with the sensitizing ovalbumin (OVA), the development of allergic airway inflammation was reduced, with decreased airway hyperresponsiveness associated with reduced peribronchial and perivascular cellular infiltration, reduced production of OVA-specific Th2 cytokines in lungs and spleens and reduced levels of serum OVA-specific IgG1 as well as IgE-dependent basophil degranulation. Of note, TLA retained its immunomodulatory properties, inducing high levels of IL-6, TNFα, IL-10 and IL-12p70 in bone marrow-derived dendritic cells after heat-inactivation or proteinase K-treatment for disruption of proteins, but not after sodium metaperiodate-treatment that degrades carbohydrate structures, suggesting that carbohydrates may play a role in immunomodulatory properties of TLA. Here we show that extracts derived from parasites may replicate the benefits of parasitic infection, offering new therapies for immune-mediated disorders.https://doi.org/10.1038/s41598-017-15663-4 |
| spellingShingle | Mirjana Drinić Angelika Wagner Priya Sarate Christian Zwicker Elke Korb Gerhard Loupal Roman Peschke Anja Joachim Ursula Wiedermann Irma Schabussova Toxoplasma gondii tachyzoite-extract acts as a potent immunomodulator against allergic sensitization and airway inflammation Scientific Reports |
| title | Toxoplasma gondii tachyzoite-extract acts as a potent immunomodulator against allergic sensitization and airway inflammation |
| title_full | Toxoplasma gondii tachyzoite-extract acts as a potent immunomodulator against allergic sensitization and airway inflammation |
| title_fullStr | Toxoplasma gondii tachyzoite-extract acts as a potent immunomodulator against allergic sensitization and airway inflammation |
| title_full_unstemmed | Toxoplasma gondii tachyzoite-extract acts as a potent immunomodulator against allergic sensitization and airway inflammation |
| title_short | Toxoplasma gondii tachyzoite-extract acts as a potent immunomodulator against allergic sensitization and airway inflammation |
| title_sort | toxoplasma gondii tachyzoite extract acts as a potent immunomodulator against allergic sensitization and airway inflammation |
| url | https://doi.org/10.1038/s41598-017-15663-4 |
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