Identification of Putative Parasitism Genes Expressed in the Esophageal Gland Cells of the Soybean Cyst Nematode Heterodera glycines
Cloning parasitism genes encoding secretory proteins expressed in the esophageal gland cells is the key to understanding the molecular basis of nematode parasitism of plants. Suppression subtractive hybridization (SSH) with the microaspirated contents from Heterodera glycines esophageal gland cells...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2001-10-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.10.1247 |
| _version_ | 1818915017350709248 |
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| author | Bingli Gao R. Allen Tom Maier Eric L. Davis Thomas J. Baum Richard S. Hussey |
| author_facet | Bingli Gao R. Allen Tom Maier Eric L. Davis Thomas J. Baum Richard S. Hussey |
| author_sort | Bingli Gao |
| collection | DOAJ |
| description | Cloning parasitism genes encoding secretory proteins expressed in the esophageal gland cells is the key to understanding the molecular basis of nematode parasitism of plants. Suppression subtractive hybridization (SSH) with the microaspirated contents from Heterodera glycines esophageal gland cells and intestinal region was used to isolate genes expressed preferentially in the gland cells of parasitic stages. Twenty-three unique cDNA sequences from a SSH cDNA library were identified and hybridized to the genomic DNA of H. glycines in Southern blots. Full-length cDNAs of 21 clones were obtained by screening a gland-cell long-distance polymerase chain reaction cDNA library. Deduced proteins of 10 clones were preceded by a signal peptide for secretion, and PSORT II computer analysis predicted eight proteins as extracellular, one as nuclear, and one as plasmalemma localized. In situ hybridization showed that four of the predicted extracellular clones were expressed specifically in the dorsal gland cell, one in the subventral gland cells, and three in the intestine in H. glycines. The predicted nuclear clone and the plasmalemma-localized clone were expressed in the subventral gland cells and the dorsal gland cell, respectively. SSH is an efficient method for cloning putative parasitism genes encoding esophageal gland cell secretory proteins that may have a role in H. glycines parasitism of soybean. |
| first_indexed | 2024-12-19T23:55:35Z |
| format | Article |
| id | doaj.art-9d9a914ece9d4fdb8fbd5a58e33a7719 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-19T23:55:35Z |
| publishDate | 2001-10-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-9d9a914ece9d4fdb8fbd5a58e33a77192022-12-21T20:01:01ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062001-10-0114101247125410.1094/MPMI.2001.14.10.1247Identification of Putative Parasitism Genes Expressed in the Esophageal Gland Cells of the Soybean Cyst Nematode Heterodera glycinesBingli GaoR. AllenTom MaierEric L. DavisThomas J. BaumRichard S. HusseyCloning parasitism genes encoding secretory proteins expressed in the esophageal gland cells is the key to understanding the molecular basis of nematode parasitism of plants. Suppression subtractive hybridization (SSH) with the microaspirated contents from Heterodera glycines esophageal gland cells and intestinal region was used to isolate genes expressed preferentially in the gland cells of parasitic stages. Twenty-three unique cDNA sequences from a SSH cDNA library were identified and hybridized to the genomic DNA of H. glycines in Southern blots. Full-length cDNAs of 21 clones were obtained by screening a gland-cell long-distance polymerase chain reaction cDNA library. Deduced proteins of 10 clones were preceded by a signal peptide for secretion, and PSORT II computer analysis predicted eight proteins as extracellular, one as nuclear, and one as plasmalemma localized. In situ hybridization showed that four of the predicted extracellular clones were expressed specifically in the dorsal gland cell, one in the subventral gland cells, and three in the intestine in H. glycines. The predicted nuclear clone and the plasmalemma-localized clone were expressed in the subventral gland cells and the dorsal gland cell, respectively. SSH is an efficient method for cloning putative parasitism genes encoding esophageal gland cell secretory proteins that may have a role in H. glycines parasitism of soybean.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.10.1247macroarrayplant-parasitic nematodesedentary plant parasitesstylet secretions |
| spellingShingle | Bingli Gao R. Allen Tom Maier Eric L. Davis Thomas J. Baum Richard S. Hussey Identification of Putative Parasitism Genes Expressed in the Esophageal Gland Cells of the Soybean Cyst Nematode Heterodera glycines Molecular Plant-Microbe Interactions macroarray plant-parasitic nematode sedentary plant parasites stylet secretions |
| title | Identification of Putative Parasitism Genes Expressed in the Esophageal Gland Cells of the Soybean Cyst Nematode Heterodera glycines |
| title_full | Identification of Putative Parasitism Genes Expressed in the Esophageal Gland Cells of the Soybean Cyst Nematode Heterodera glycines |
| title_fullStr | Identification of Putative Parasitism Genes Expressed in the Esophageal Gland Cells of the Soybean Cyst Nematode Heterodera glycines |
| title_full_unstemmed | Identification of Putative Parasitism Genes Expressed in the Esophageal Gland Cells of the Soybean Cyst Nematode Heterodera glycines |
| title_short | Identification of Putative Parasitism Genes Expressed in the Esophageal Gland Cells of the Soybean Cyst Nematode Heterodera glycines |
| title_sort | identification of putative parasitism genes expressed in the esophageal gland cells of the soybean cyst nematode heterodera glycines |
| topic | macroarray plant-parasitic nematode sedentary plant parasites stylet secretions |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.10.1247 |
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