Antimicrobial and Cytotoxic Activities of the Secondary Metabolites of Endophytic Fungi Isolated from the Medicinal Plant <i>Hyssopus officinalis</i>
According to the World Health Organization, it is estimated that by 2050, drug-resistant infections could cause up to 10 million deaths annually. Therefore, finding a new generation of antibiotics is crucial. Natural compounds from endophytic fungi are considered a potential source of new-generation...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/7/1201 |
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| author | Farkhod Eshboev Marina Karakozova Jaloliddin Abdurakhmanov Khayrulla Bobakulov Khayotjon Dolimov Akhror Abdurashidov Asadali Baymirzaev Artyom Makhnyov Ekaterina Terenteva Sobirdjan Sasmakov Galina Piyakina Dilfuza Egamberdieva Pavel A. Nazarov Shakhnoz Azimova |
| author_facet | Farkhod Eshboev Marina Karakozova Jaloliddin Abdurakhmanov Khayrulla Bobakulov Khayotjon Dolimov Akhror Abdurashidov Asadali Baymirzaev Artyom Makhnyov Ekaterina Terenteva Sobirdjan Sasmakov Galina Piyakina Dilfuza Egamberdieva Pavel A. Nazarov Shakhnoz Azimova |
| author_sort | Farkhod Eshboev |
| collection | DOAJ |
| description | According to the World Health Organization, it is estimated that by 2050, drug-resistant infections could cause up to 10 million deaths annually. Therefore, finding a new generation of antibiotics is crucial. Natural compounds from endophytic fungi are considered a potential source of new-generation antibiotics. The antimicrobial and cytotoxic effects of ethyl acetate extracts of nine endophytic fungal isolates obtained from <i>Hyssopus officinalis</i> were investigated for bioassay-guided isolation of the natural compounds. An extract of isolate VII showed the highest antimicrobial activities against Gram-positive bacteria <i>Bacillus subtilis</i> and <i>Staphylococcus aureus</i> (30.12 ± 0.20 mm and 35.21 ± 0.20 mm) and Gram-negative bacteria <i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i> (30.41 ± 0.23 mm and 25.12 ± 0.25 mm) among the tested extracts of isolates. Molecular identification of isolate VII confirmed it as <i>Chaetomium elatum</i> based on sequencing of its ITS genes, and it was discovered that this was the first time <i>C. elatum</i> had been isolated from <i>H. officinalis.</i> This isolate was cultured at a large scale for the isolation and identification of the active compound. Penicillic acid was isolated for the first time from <i>C. elatum</i> and its chemical structure was established by NMR spectroscopy. The penicillic acid showed strong antibacterial activities against <i>Bacillus subtilis</i> and <i>Staphylococcus aureus</i> with 20.68 mm and 25.51 mm inhibition zones, respectively. In addition, MIC and MBC values and antibiofilm activities of penicillic acid were determined. It was found that penicillic acid reduced the level of biofilms in proportion to antibacterial activity. |
| first_indexed | 2024-03-11T01:21:48Z |
| format | Article |
| id | doaj.art-9d9b564bd98b4c398f846f5990426a3f |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T01:21:48Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-9d9b564bd98b4c398f846f5990426a3f2023-11-18T18:03:42ZengMDPI AGAntibiotics2079-63822023-07-01127120110.3390/antibiotics12071201Antimicrobial and Cytotoxic Activities of the Secondary Metabolites of Endophytic Fungi Isolated from the Medicinal Plant <i>Hyssopus officinalis</i>Farkhod Eshboev0Marina Karakozova1Jaloliddin Abdurakhmanov2Khayrulla Bobakulov3Khayotjon Dolimov4Akhror Abdurashidov5Asadali Baymirzaev6Artyom Makhnyov7Ekaterina Terenteva8Sobirdjan Sasmakov9Galina Piyakina10Dilfuza Egamberdieva11Pavel A. Nazarov12Shakhnoz Azimova13S.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, 77 Mirzo Ulugbek Str., Tashkent 100170, UzbekistanBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, RussiaS.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, 77 Mirzo Ulugbek Str., Tashkent 100170, UzbekistanS.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, 77 Mirzo Ulugbek Str., Tashkent 100170, UzbekistanS.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, 77 Mirzo Ulugbek Str., Tashkent 100170, UzbekistanS.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, 77 Mirzo Ulugbek Str., Tashkent 100170, UzbekistanS.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, 77 Mirzo Ulugbek Str., Tashkent 100170, UzbekistanS.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, 77 Mirzo Ulugbek Str., Tashkent 100170, UzbekistanS.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, 77 Mirzo Ulugbek Str., Tashkent 100170, UzbekistanS.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, 77 Mirzo Ulugbek Str., Tashkent 100170, UzbekistanS.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, 77 Mirzo Ulugbek Str., Tashkent 100170, Uzbekistan“Tashkent Institute of Irrigation and Agricultural Mechanization Engineers” National Research University, 39 Kori Niyoziy Str., Tashkent 100000, UzbekistanBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, RussiaS.Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, 77 Mirzo Ulugbek Str., Tashkent 100170, UzbekistanAccording to the World Health Organization, it is estimated that by 2050, drug-resistant infections could cause up to 10 million deaths annually. Therefore, finding a new generation of antibiotics is crucial. Natural compounds from endophytic fungi are considered a potential source of new-generation antibiotics. The antimicrobial and cytotoxic effects of ethyl acetate extracts of nine endophytic fungal isolates obtained from <i>Hyssopus officinalis</i> were investigated for bioassay-guided isolation of the natural compounds. An extract of isolate VII showed the highest antimicrobial activities against Gram-positive bacteria <i>Bacillus subtilis</i> and <i>Staphylococcus aureus</i> (30.12 ± 0.20 mm and 35.21 ± 0.20 mm) and Gram-negative bacteria <i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i> (30.41 ± 0.23 mm and 25.12 ± 0.25 mm) among the tested extracts of isolates. Molecular identification of isolate VII confirmed it as <i>Chaetomium elatum</i> based on sequencing of its ITS genes, and it was discovered that this was the first time <i>C. elatum</i> had been isolated from <i>H. officinalis.</i> This isolate was cultured at a large scale for the isolation and identification of the active compound. Penicillic acid was isolated for the first time from <i>C. elatum</i> and its chemical structure was established by NMR spectroscopy. The penicillic acid showed strong antibacterial activities against <i>Bacillus subtilis</i> and <i>Staphylococcus aureus</i> with 20.68 mm and 25.51 mm inhibition zones, respectively. In addition, MIC and MBC values and antibiofilm activities of penicillic acid were determined. It was found that penicillic acid reduced the level of biofilms in proportion to antibacterial activity.https://www.mdpi.com/2079-6382/12/7/1201antibiofilm activityantimicrobial activity<i>Chaetomium elatum</i>cytotoxic activityendophytic fungi<i>Hyssopus officinalis</i> |
| spellingShingle | Farkhod Eshboev Marina Karakozova Jaloliddin Abdurakhmanov Khayrulla Bobakulov Khayotjon Dolimov Akhror Abdurashidov Asadali Baymirzaev Artyom Makhnyov Ekaterina Terenteva Sobirdjan Sasmakov Galina Piyakina Dilfuza Egamberdieva Pavel A. Nazarov Shakhnoz Azimova Antimicrobial and Cytotoxic Activities of the Secondary Metabolites of Endophytic Fungi Isolated from the Medicinal Plant <i>Hyssopus officinalis</i> Antibiotics antibiofilm activity antimicrobial activity <i>Chaetomium elatum</i> cytotoxic activity endophytic fungi <i>Hyssopus officinalis</i> |
| title | Antimicrobial and Cytotoxic Activities of the Secondary Metabolites of Endophytic Fungi Isolated from the Medicinal Plant <i>Hyssopus officinalis</i> |
| title_full | Antimicrobial and Cytotoxic Activities of the Secondary Metabolites of Endophytic Fungi Isolated from the Medicinal Plant <i>Hyssopus officinalis</i> |
| title_fullStr | Antimicrobial and Cytotoxic Activities of the Secondary Metabolites of Endophytic Fungi Isolated from the Medicinal Plant <i>Hyssopus officinalis</i> |
| title_full_unstemmed | Antimicrobial and Cytotoxic Activities of the Secondary Metabolites of Endophytic Fungi Isolated from the Medicinal Plant <i>Hyssopus officinalis</i> |
| title_short | Antimicrobial and Cytotoxic Activities of the Secondary Metabolites of Endophytic Fungi Isolated from the Medicinal Plant <i>Hyssopus officinalis</i> |
| title_sort | antimicrobial and cytotoxic activities of the secondary metabolites of endophytic fungi isolated from the medicinal plant i hyssopus officinalis i |
| topic | antibiofilm activity antimicrobial activity <i>Chaetomium elatum</i> cytotoxic activity endophytic fungi <i>Hyssopus officinalis</i> |
| url | https://www.mdpi.com/2079-6382/12/7/1201 |
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