Validation of aqueous two-phase extraction method
Nowadays, consumer interest in food with natural ingredients has increased. This need has led to the research of new sources and green extraction methods. Betalains are compounds responsible for giving color to cacti fruits. The aim is to obtain low-sugar betacyanins extracts from jiotilla Escontria...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-01-01
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| Series: | MethodsX |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016121002144 |
| _version_ | 1819099445647638528 |
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| author | Luisaldo Sandate-Flores José Rodríguez-Rodríguez Magdalena Rostro-Alanis Jorge Alejandro Santiago Urbina Karla Mayolo-Deloisa Elda M. Melchor-Martínez Juan Eduardo Sosa-Hernández Roberto Parra-Saldívar Hafiz M.N. Iqbal |
| author_facet | Luisaldo Sandate-Flores José Rodríguez-Rodríguez Magdalena Rostro-Alanis Jorge Alejandro Santiago Urbina Karla Mayolo-Deloisa Elda M. Melchor-Martínez Juan Eduardo Sosa-Hernández Roberto Parra-Saldívar Hafiz M.N. Iqbal |
| author_sort | Luisaldo Sandate-Flores |
| collection | DOAJ |
| description | Nowadays, consumer interest in food with natural ingredients has increased. This need has led to the research of new sources and green extraction methods. Betalains are compounds responsible for giving color to cacti fruits. The aim is to obtain low-sugar betacyanins extracts from jiotilla Escontria chiotilla using aqueous two-phase systems (ATPS) to color food with the extract. The effect of principal parameters of ATPS (Ethyl alcohol- KH2PO4/K2HPO4) as tie-line length (TL;40,50 and 70), phase volume ratios (Vr; 1 and 3) on the partitioning of betacyanins, betaxanthins, total sugars, reducing sugars, and antioxidant activity in the extract was evaluated. The yields were determined from the top and bottom phases of the aforementioned parameters. Multivariate analysis of variance (MANOVA, α = 0.05) showed that TLL and Vr were statistically significant (P < 0.05). The lowest bottom sugar yield (25.78 ± 3.14%) corresponds to TLL = 40, Vr = 3. Under these conditions, the corresponding value for betacyanins yield is 62.98±4.52%. For the first time, the ATPS was used to extract betacyanins from cactus fruit. • Escontria chiotilla, as a biological source, contained a high percent of betalains • Aqueous two-phase systems (ATPS) was statistically optimized • The developed method enriches the valorization of environmentally related plants waste materials |
| first_indexed | 2024-12-22T00:47:00Z |
| format | Article |
| id | doaj.art-9d9c987d5fdb4237837ed110ac2982f3 |
| institution | Directory Open Access Journal |
| issn | 2215-0161 |
| language | English |
| last_indexed | 2024-12-22T00:47:00Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj.art-9d9c987d5fdb4237837ed110ac2982f32022-12-21T18:44:32ZengElsevierMethodsX2215-01612021-01-018101421Validation of aqueous two-phase extraction methodLuisaldo Sandate-Flores0José Rodríguez-Rodríguez1Magdalena Rostro-Alanis2Jorge Alejandro Santiago Urbina3Karla Mayolo-Deloisa4Elda M. Melchor-Martínez5Juan Eduardo Sosa-Hernández6Roberto Parra-Saldívar7Hafiz M.N. Iqbal8Tecnologico de Monterrey, School of Engineering and Sciences, Monterrey 64849 MexicoTecnologico de Monterrey, School of Engineering and Sciences, Monterrey 64849 MexicoTecnologico de Monterrey, School of Engineering and Sciences, Monterrey 64849 MexicoUniversidad Tecnológica de los Valles Centrales de Oaxaca, Avenida Universidad S/N, San Pablo Huixtepec, Zimatlán de Álvarez, Oaxaca 71270, MexicoTecnologico de Monterrey, School of Engineering and Sciences, Monterrey 64849 MexicoTecnologico de Monterrey, School of Engineering and Sciences, Monterrey 64849 MexicoTecnologico de Monterrey, School of Engineering and Sciences, Monterrey 64849 MexicoTecnologico de Monterrey, School of Engineering and Sciences, Monterrey 64849 Mexico; Corresponding authors.Tecnologico de Monterrey, School of Engineering and Sciences, Monterrey 64849 Mexico; Corresponding authors.Nowadays, consumer interest in food with natural ingredients has increased. This need has led to the research of new sources and green extraction methods. Betalains are compounds responsible for giving color to cacti fruits. The aim is to obtain low-sugar betacyanins extracts from jiotilla Escontria chiotilla using aqueous two-phase systems (ATPS) to color food with the extract. The effect of principal parameters of ATPS (Ethyl alcohol- KH2PO4/K2HPO4) as tie-line length (TL;40,50 and 70), phase volume ratios (Vr; 1 and 3) on the partitioning of betacyanins, betaxanthins, total sugars, reducing sugars, and antioxidant activity in the extract was evaluated. The yields were determined from the top and bottom phases of the aforementioned parameters. Multivariate analysis of variance (MANOVA, α = 0.05) showed that TLL and Vr were statistically significant (P < 0.05). The lowest bottom sugar yield (25.78 ± 3.14%) corresponds to TLL = 40, Vr = 3. Under these conditions, the corresponding value for betacyanins yield is 62.98±4.52%. For the first time, the ATPS was used to extract betacyanins from cactus fruit. • Escontria chiotilla, as a biological source, contained a high percent of betalains • Aqueous two-phase systems (ATPS) was statistically optimized • The developed method enriches the valorization of environmentally related plants waste materialshttp://www.sciencedirect.com/science/article/pii/S2215016121002144Aqueous two-phase systems (ATPS) |
| spellingShingle | Luisaldo Sandate-Flores José Rodríguez-Rodríguez Magdalena Rostro-Alanis Jorge Alejandro Santiago Urbina Karla Mayolo-Deloisa Elda M. Melchor-Martínez Juan Eduardo Sosa-Hernández Roberto Parra-Saldívar Hafiz M.N. Iqbal Validation of aqueous two-phase extraction method MethodsX Aqueous two-phase systems (ATPS) |
| title | Validation of aqueous two-phase extraction method |
| title_full | Validation of aqueous two-phase extraction method |
| title_fullStr | Validation of aqueous two-phase extraction method |
| title_full_unstemmed | Validation of aqueous two-phase extraction method |
| title_short | Validation of aqueous two-phase extraction method |
| title_sort | validation of aqueous two phase extraction method |
| topic | Aqueous two-phase systems (ATPS) |
| url | http://www.sciencedirect.com/science/article/pii/S2215016121002144 |
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