THE GENERATION OF REACTIVE OXYGEN SPECIES BY CORD BLOOD NUCLEATED CELLS DURING CRYOPRESERVATION
The generation of reactive oxygen species in different populations of nucleated cells during cord blood cryopreservation was studied. Using cytometry with the 2′,7′-dichlorodihydrofluorescein diacetate dye we assessed the integral content of reactive oxygen species in various populations (lymphocyte...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2014-08-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/storage/4_2014/riazantsev.pdf |
| _version_ | 1797762805832613888 |
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| author | Ryazantsev V. V. Babijchuk L. A. Mykhailova O. O. Zubov P. M. |
| author_facet | Ryazantsev V. V. Babijchuk L. A. Mykhailova O. O. Zubov P. M. |
| author_sort | Ryazantsev V. V. |
| collection | DOAJ |
| description | The generation of reactive oxygen species in different populations of nucleated cells during cord blood cryopreservation was studied. Using cytometry with the 2′,7′-dichlorodihydrofluorescein diacetate dye we assessed the integral content of reactive oxygen species in various populations (lymphocytes, monocytes, granulocytes) of cord blood nucleated cells, depending on the method of isolation, treatment with cryoprotectant and freezing. Furthermore the activity of antioxidant enzymes prior to and after cryopreservation while the cells were loaded with exogenous reactive oxygen species, which source was hydrogen peroxide, was assessed. The results obtained showed that the isolation and cryopreservation of cord blood nucleated cells according to proposed method, which included the isolation of cells in polyglucinum and freezing with 5% DMSO did not result in a significant increase in level of intracellular reactive oxygen species. In addition the activity of antioxidant enzymes to exogenous hydrogen peroxide inactivation in cryopreserved cell was maintained at the level close to that in the native cord blood. Among all the populations of cord blood nucleated cells the granulocytes were the least resistant to cryopreservation effects. |
| first_indexed | 2024-03-12T19:32:44Z |
| format | Article |
| id | doaj.art-9d9d0caaacef439586a984fa3309a08f |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T19:32:44Z |
| publishDate | 2014-08-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-9d9d0caaacef439586a984fa3309a08f2023-08-02T04:24:24ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2014-08-017410010610.15407/biotech7.04.100THE GENERATION OF REACTIVE OXYGEN SPECIES BY CORD BLOOD NUCLEATED CELLS DURING CRYOPRESERVATIONRyazantsev V. V.0Babijchuk L. A.1Mykhailova O. O.2Zubov P. M.3Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, KharkivInstitute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, KharkivInstitute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, KharkivInstitute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, KharkivThe generation of reactive oxygen species in different populations of nucleated cells during cord blood cryopreservation was studied. Using cytometry with the 2′,7′-dichlorodihydrofluorescein diacetate dye we assessed the integral content of reactive oxygen species in various populations (lymphocytes, monocytes, granulocytes) of cord blood nucleated cells, depending on the method of isolation, treatment with cryoprotectant and freezing. Furthermore the activity of antioxidant enzymes prior to and after cryopreservation while the cells were loaded with exogenous reactive oxygen species, which source was hydrogen peroxide, was assessed. The results obtained showed that the isolation and cryopreservation of cord blood nucleated cells according to proposed method, which included the isolation of cells in polyglucinum and freezing with 5% DMSO did not result in a significant increase in level of intracellular reactive oxygen species. In addition the activity of antioxidant enzymes to exogenous hydrogen peroxide inactivation in cryopreserved cell was maintained at the level close to that in the native cord blood. Among all the populations of cord blood nucleated cells the granulocytes were the least resistant to cryopreservation effects.http://biotechnology.kiev.ua/images/storage/4_2014/riazantsev.pdfnucleated cellscord bloodcryopreservationreactive oxygen species. |
| spellingShingle | Ryazantsev V. V. Babijchuk L. A. Mykhailova O. O. Zubov P. M. THE GENERATION OF REACTIVE OXYGEN SPECIES BY CORD BLOOD NUCLEATED CELLS DURING CRYOPRESERVATION Biotechnologia Acta nucleated cells cord blood cryopreservation reactive oxygen species. |
| title | THE GENERATION OF REACTIVE OXYGEN SPECIES BY CORD BLOOD NUCLEATED CELLS DURING CRYOPRESERVATION |
| title_full | THE GENERATION OF REACTIVE OXYGEN SPECIES BY CORD BLOOD NUCLEATED CELLS DURING CRYOPRESERVATION |
| title_fullStr | THE GENERATION OF REACTIVE OXYGEN SPECIES BY CORD BLOOD NUCLEATED CELLS DURING CRYOPRESERVATION |
| title_full_unstemmed | THE GENERATION OF REACTIVE OXYGEN SPECIES BY CORD BLOOD NUCLEATED CELLS DURING CRYOPRESERVATION |
| title_short | THE GENERATION OF REACTIVE OXYGEN SPECIES BY CORD BLOOD NUCLEATED CELLS DURING CRYOPRESERVATION |
| title_sort | generation of reactive oxygen species by cord blood nucleated cells during cryopreservation |
| topic | nucleated cells cord blood cryopreservation reactive oxygen species. |
| url | http://biotechnology.kiev.ua/images/storage/4_2014/riazantsev.pdf |
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