Isolation and characterization of marine bacteria from East Coast of India: functional screening for salt stress tolerance

Soil salinization has become a severe constraint for crop production world-wide which necessitated development or induced enhancement of salt stress tolerance in plant life to sustain production in saline lands. Recognition and prospecting of valuable stress tolerant genes from natural microbial res...

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Main Authors: Priyanka Das, Soumendranath Chatterjee, Bijay Kumar Behera, Tushar Kanti Dangar, Basanta Kumar Das, Trilochan Mohapatra
Format: Article
Language:English
Published: Elsevier 2019-06-01
Series:Heliyon
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2405844019334000
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author Priyanka Das
Soumendranath Chatterjee
Bijay Kumar Behera
Tushar Kanti Dangar
Basanta Kumar Das
Trilochan Mohapatra
author_facet Priyanka Das
Soumendranath Chatterjee
Bijay Kumar Behera
Tushar Kanti Dangar
Basanta Kumar Das
Trilochan Mohapatra
author_sort Priyanka Das
collection DOAJ
description Soil salinization has become a severe constraint for crop production world-wide which necessitated development or induced enhancement of salt stress tolerance in plant life to sustain production in saline lands. Recognition and prospecting of valuable stress tolerant genes from natural microbial resources of saline habitat is obscure to date. Therefore, the investigation was towards isolation and characterization of marine salt stress tolerant microbes along the East coast of India for revelation of effective salt stress tolerant genes. Salt stress tolerance was assessed from 98 bacterial isolates obtained from 28 water and soil samples. Among them, 35 isolates which failed to grow beyond 4% salt were discarded and remainder 63 isolates were selected for further functional analysis and only seven isolates recorded ≥8% NaCl stress tolerance. Phylogeny revealed that four isolates belong to Firmicutes and three isolates were members of Proteobacteria. Ribosomal Database Project Release-11 and SILVA SSU database based genotyping and taxonomic identity analysis confirmed that the higher (20%) salt stress tolerant bacteria were Staphylococcus sp., Enterococcus sp., Enterobacter sp. and Proteus sp. To investigate candidate, as well as, novel salt stress tolerant genes, the seven bacterial isolates would provide new horizon to focus on the recent developments of salinity stress tolerance. In addition, the findings evidently point out the diversity of salt stress tolerant marine bacteria in coastal Odisha and West Bengal, India.
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spelling doaj.art-9d9f478716b84965af9af136d86c8cf82022-12-22T00:45:51ZengElsevierHeliyon2405-84402019-06-0156e01869Isolation and characterization of marine bacteria from East Coast of India: functional screening for salt stress tolerancePriyanka Das0Soumendranath Chatterjee1Bijay Kumar Behera2Tushar Kanti Dangar3Basanta Kumar Das4Trilochan Mohapatra5Biotechnology Laboratory, Fishery Resource and Environmental Management Division, ICAR- Central Inland Fisheries Research Institute, Barrackpore, West Bengal, India; Parasitology and Microbiology Research Laboratory, Department of Zoology, University of Burdwan, Burdwan, West Bengal, IndiaParasitology and Microbiology Research Laboratory, Department of Zoology, University of Burdwan, Burdwan, West Bengal, IndiaBiotechnology Laboratory, Fishery Resource and Environmental Management Division, ICAR- Central Inland Fisheries Research Institute, Barrackpore, West Bengal, India; Corresponding author.Microbiology Laboratory, Crop Production Division, ICAR-National Rice Research Institute, Cuttack, Odisha, IndiaBiotechnology Laboratory, Fishery Resource and Environmental Management Division, ICAR- Central Inland Fisheries Research Institute, Barrackpore, West Bengal, IndiaDirector General, Indian Council of Agricultural Research, New Delhi, IndiaSoil salinization has become a severe constraint for crop production world-wide which necessitated development or induced enhancement of salt stress tolerance in plant life to sustain production in saline lands. Recognition and prospecting of valuable stress tolerant genes from natural microbial resources of saline habitat is obscure to date. Therefore, the investigation was towards isolation and characterization of marine salt stress tolerant microbes along the East coast of India for revelation of effective salt stress tolerant genes. Salt stress tolerance was assessed from 98 bacterial isolates obtained from 28 water and soil samples. Among them, 35 isolates which failed to grow beyond 4% salt were discarded and remainder 63 isolates were selected for further functional analysis and only seven isolates recorded ≥8% NaCl stress tolerance. Phylogeny revealed that four isolates belong to Firmicutes and three isolates were members of Proteobacteria. Ribosomal Database Project Release-11 and SILVA SSU database based genotyping and taxonomic identity analysis confirmed that the higher (20%) salt stress tolerant bacteria were Staphylococcus sp., Enterococcus sp., Enterobacter sp. and Proteus sp. To investigate candidate, as well as, novel salt stress tolerant genes, the seven bacterial isolates would provide new horizon to focus on the recent developments of salinity stress tolerance. In addition, the findings evidently point out the diversity of salt stress tolerant marine bacteria in coastal Odisha and West Bengal, India.http://www.sciencedirect.com/science/article/pii/S2405844019334000BioinformaticsBiotechnologyMicrobiologyMolecular biology
spellingShingle Priyanka Das
Soumendranath Chatterjee
Bijay Kumar Behera
Tushar Kanti Dangar
Basanta Kumar Das
Trilochan Mohapatra
Isolation and characterization of marine bacteria from East Coast of India: functional screening for salt stress tolerance
Heliyon
Bioinformatics
Biotechnology
Microbiology
Molecular biology
title Isolation and characterization of marine bacteria from East Coast of India: functional screening for salt stress tolerance
title_full Isolation and characterization of marine bacteria from East Coast of India: functional screening for salt stress tolerance
title_fullStr Isolation and characterization of marine bacteria from East Coast of India: functional screening for salt stress tolerance
title_full_unstemmed Isolation and characterization of marine bacteria from East Coast of India: functional screening for salt stress tolerance
title_short Isolation and characterization of marine bacteria from East Coast of India: functional screening for salt stress tolerance
title_sort isolation and characterization of marine bacteria from east coast of india functional screening for salt stress tolerance
topic Bioinformatics
Biotechnology
Microbiology
Molecular biology
url http://www.sciencedirect.com/science/article/pii/S2405844019334000
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