A new multiplex polymerase chain reaction assay for the identification a panel of bacteria involved in bacteremia
Background: Throughout the world, bloodstream infections (BSI s ) are associated with high rates of morbidity and mortality. Rapid pathogens identification is central significance for the outcome of the patient than culture techniques for microbial identification. To develop an end point multiplex P...
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| Format: | Article |
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Wolters Kluwer Medknow Publications
2013-01-01
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| Series: | Advanced Biomedical Research |
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| Online Access: | http://www.advbiores.net/article.asp?issn=2277-9175;year=2013;volume=2;issue=1;spage=7;epage=7;aulast=Fazzeli |
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| author | Hossein Fazzeli Mohammad R Arabestani Bahram N Esfahani Farzin Khorvash Mohammad R Pourshafie Sharareh Moghim Hajieh G Safaei Jamshid Faghri Amir Azimian |
| author_facet | Hossein Fazzeli Mohammad R Arabestani Bahram N Esfahani Farzin Khorvash Mohammad R Pourshafie Sharareh Moghim Hajieh G Safaei Jamshid Faghri Amir Azimian |
| author_sort | Hossein Fazzeli |
| collection | DOAJ |
| description | Background: Throughout the world, bloodstream infections (BSI s ) are associated with high rates of morbidity and mortality. Rapid pathogens identification is central significance for the outcome of the patient than culture techniques for microbial identification. To develop an end point multiplex PCR to identify a group of bacteria including Enterococcus spp., Pseudomons aeruginosa, Staphylococcus spp., Acinetobacter baumannii, 16S rDNA, and Drosophila Melanogaster were used as internal control (IC).
Materials and Methods: Design of primers was done using Mega4, Allel ID6, Oligo6 and Oligo analyzer softwares. Genetic targets for primer designing and identification of genus Enterococcus spp., Staphylococcus spp., and species of Acinetobacter baumannii, Pseudomons aeruginosa, included the rpoB, rpoB and gyrA, sss respectively. Then PCR and multiplex PCR were performed
Results: The intended specificity was obtained for the bacteria, which used in this study and there wasn′t seen any unspecific amplification by the multiplex PCR. The test showed a sensitivity ranging from 1 to 100 target copies per reaction depending on the bacterial species.
Conclusions: The presented multiplex PCR offers a rapid and accurate molecular diagnostic tool for simultaneous detection of some pathogenic microorganisms. The IC exists in the multiplex PCR accompanied by other primers in the system, can serve as a simple, cost- effective internal control for the multiplex PCR assay. |
| first_indexed | 2024-12-11T00:24:19Z |
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| issn | 2277-9175 2277-9175 |
| language | English |
| last_indexed | 2024-12-11T00:24:19Z |
| publishDate | 2013-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
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| series | Advanced Biomedical Research |
| spelling | doaj.art-9da661a5d2af402b9d04c3fa0c39896d2022-12-22T01:27:37ZengWolters Kluwer Medknow PublicationsAdvanced Biomedical Research2277-91752277-91752013-01-01217710.4103/2277-9175.107972A new multiplex polymerase chain reaction assay for the identification a panel of bacteria involved in bacteremiaHossein FazzeliMohammad R ArabestaniBahram N EsfahaniFarzin KhorvashMohammad R PourshafieSharareh MoghimHajieh G SafaeiJamshid FaghriAmir AzimianBackground: Throughout the world, bloodstream infections (BSI s ) are associated with high rates of morbidity and mortality. Rapid pathogens identification is central significance for the outcome of the patient than culture techniques for microbial identification. To develop an end point multiplex PCR to identify a group of bacteria including Enterococcus spp., Pseudomons aeruginosa, Staphylococcus spp., Acinetobacter baumannii, 16S rDNA, and Drosophila Melanogaster were used as internal control (IC). Materials and Methods: Design of primers was done using Mega4, Allel ID6, Oligo6 and Oligo analyzer softwares. Genetic targets for primer designing and identification of genus Enterococcus spp., Staphylococcus spp., and species of Acinetobacter baumannii, Pseudomons aeruginosa, included the rpoB, rpoB and gyrA, sss respectively. Then PCR and multiplex PCR were performed Results: The intended specificity was obtained for the bacteria, which used in this study and there wasn′t seen any unspecific amplification by the multiplex PCR. The test showed a sensitivity ranging from 1 to 100 target copies per reaction depending on the bacterial species. Conclusions: The presented multiplex PCR offers a rapid and accurate molecular diagnostic tool for simultaneous detection of some pathogenic microorganisms. The IC exists in the multiplex PCR accompanied by other primers in the system, can serve as a simple, cost- effective internal control for the multiplex PCR assay.http://www.advbiores.net/article.asp?issn=2277-9175;year=2013;volume=2;issue=1;spage=7;epage=7;aulast=FazzeliConventional multiplex polymerase chain reactionprimers designbacterimia |
| spellingShingle | Hossein Fazzeli Mohammad R Arabestani Bahram N Esfahani Farzin Khorvash Mohammad R Pourshafie Sharareh Moghim Hajieh G Safaei Jamshid Faghri Amir Azimian A new multiplex polymerase chain reaction assay for the identification a panel of bacteria involved in bacteremia Advanced Biomedical Research Conventional multiplex polymerase chain reaction primers design bacterimia |
| title | A new multiplex polymerase chain reaction assay for the identification a panel of bacteria involved in bacteremia |
| title_full | A new multiplex polymerase chain reaction assay for the identification a panel of bacteria involved in bacteremia |
| title_fullStr | A new multiplex polymerase chain reaction assay for the identification a panel of bacteria involved in bacteremia |
| title_full_unstemmed | A new multiplex polymerase chain reaction assay for the identification a panel of bacteria involved in bacteremia |
| title_short | A new multiplex polymerase chain reaction assay for the identification a panel of bacteria involved in bacteremia |
| title_sort | new multiplex polymerase chain reaction assay for the identification a panel of bacteria involved in bacteremia |
| topic | Conventional multiplex polymerase chain reaction primers design bacterimia |
| url | http://www.advbiores.net/article.asp?issn=2277-9175;year=2013;volume=2;issue=1;spage=7;epage=7;aulast=Fazzeli |
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