Zebrafish Model for Studying Dexamethasone-Induced Muscle Atrophy and Preventive Effect of Maca (<i>Lepidium meyenii</i>)
Loss of myofibers during muscle atrophy affects functional capacity and quality of life. Dexamethasone, an inducer of rapid atrophy of skeletal myofibers, has been studied as a glucocorticoid receptor in muscle atrophy or motor neurodegeneration. In this study, we examined dexamethasone-induced musc...
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MDPI AG
2021-10-01
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| Online Access: | https://www.mdpi.com/2073-4409/10/11/2879 |
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| author | Bomi Ryu Jun-Geon Je You-Jin Jeon Hye-Won Yang |
| author_facet | Bomi Ryu Jun-Geon Je You-Jin Jeon Hye-Won Yang |
| author_sort | Bomi Ryu |
| collection | DOAJ |
| description | Loss of myofibers during muscle atrophy affects functional capacity and quality of life. Dexamethasone, an inducer of rapid atrophy of skeletal myofibers, has been studied as a glucocorticoid receptor in muscle atrophy or motor neurodegeneration. In this study, we examined dexamethasone-induced muscle atrophy using zebrafish (<i>Danio rerio</i>), a vertebrate model, and assessed whether administration of <i>Lepidium meyenii</i> (maca) as a dietary supplement can prevent muscle atrophy. Changes in skeletal myofibers in zebrafish were evaluated after exposure to dexamethasone for different periods and at different concentrations. Under optimized conditions, zebrafish pre-fed with maca for 3 days were exposed to 0.01% dexamethasone for 1 h/day for 7 days. Thereafter, myofiber loss, damaged muscle contractile proteins, and abnormal exploratory behavior due to the structural and functional impairment of skeletal muscle associated with muscle atrophy were investigated using hematoxylin–eosin, immunofluorescence staining, and behavioral analyses. Our findings suggest that dexamethasone induces muscle atrophy in zebrafish, inhibiting exploratory behavior by inducing myofiber loss, inhibiting muscle contraction, and causing changes in endurance and velocity. Thus, the zebrafish model can be used to screen pharmaceutical agents and to study muscle atrophy. Furthermore, maca is a potential dietary supplement to prevent muscle atrophy, as it protects muscle fibers. |
| first_indexed | 2024-03-10T05:37:35Z |
| format | Article |
| id | doaj.art-9da6aa82e1db4a4c85779486da71aa5c |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T05:37:35Z |
| publishDate | 2021-10-01 |
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| record_format | Article |
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| spelling | doaj.art-9da6aa82e1db4a4c85779486da71aa5c2023-11-22T22:48:05ZengMDPI AGCells2073-44092021-10-011011287910.3390/cells10112879Zebrafish Model for Studying Dexamethasone-Induced Muscle Atrophy and Preventive Effect of Maca (<i>Lepidium meyenii</i>)Bomi Ryu0Jun-Geon Je1You-Jin Jeon2Hye-Won Yang3Department of Marine Life Science, Jeju National University, Jeju 63243, KoreaDepartment of Marine Life Science, Jeju National University, Jeju 63243, KoreaDepartment of Marine Life Science, Jeju National University, Jeju 63243, KoreaDepartment of Marine Life Science, Jeju National University, Jeju 63243, KoreaLoss of myofibers during muscle atrophy affects functional capacity and quality of life. Dexamethasone, an inducer of rapid atrophy of skeletal myofibers, has been studied as a glucocorticoid receptor in muscle atrophy or motor neurodegeneration. In this study, we examined dexamethasone-induced muscle atrophy using zebrafish (<i>Danio rerio</i>), a vertebrate model, and assessed whether administration of <i>Lepidium meyenii</i> (maca) as a dietary supplement can prevent muscle atrophy. Changes in skeletal myofibers in zebrafish were evaluated after exposure to dexamethasone for different periods and at different concentrations. Under optimized conditions, zebrafish pre-fed with maca for 3 days were exposed to 0.01% dexamethasone for 1 h/day for 7 days. Thereafter, myofiber loss, damaged muscle contractile proteins, and abnormal exploratory behavior due to the structural and functional impairment of skeletal muscle associated with muscle atrophy were investigated using hematoxylin–eosin, immunofluorescence staining, and behavioral analyses. Our findings suggest that dexamethasone induces muscle atrophy in zebrafish, inhibiting exploratory behavior by inducing myofiber loss, inhibiting muscle contraction, and causing changes in endurance and velocity. Thus, the zebrafish model can be used to screen pharmaceutical agents and to study muscle atrophy. Furthermore, maca is a potential dietary supplement to prevent muscle atrophy, as it protects muscle fibers.https://www.mdpi.com/2073-4409/10/11/2879zebrafishmuscle atrophydexamethasonemaca |
| spellingShingle | Bomi Ryu Jun-Geon Je You-Jin Jeon Hye-Won Yang Zebrafish Model for Studying Dexamethasone-Induced Muscle Atrophy and Preventive Effect of Maca (<i>Lepidium meyenii</i>) Cells zebrafish muscle atrophy dexamethasone maca |
| title | Zebrafish Model for Studying Dexamethasone-Induced Muscle Atrophy and Preventive Effect of Maca (<i>Lepidium meyenii</i>) |
| title_full | Zebrafish Model for Studying Dexamethasone-Induced Muscle Atrophy and Preventive Effect of Maca (<i>Lepidium meyenii</i>) |
| title_fullStr | Zebrafish Model for Studying Dexamethasone-Induced Muscle Atrophy and Preventive Effect of Maca (<i>Lepidium meyenii</i>) |
| title_full_unstemmed | Zebrafish Model for Studying Dexamethasone-Induced Muscle Atrophy and Preventive Effect of Maca (<i>Lepidium meyenii</i>) |
| title_short | Zebrafish Model for Studying Dexamethasone-Induced Muscle Atrophy and Preventive Effect of Maca (<i>Lepidium meyenii</i>) |
| title_sort | zebrafish model for studying dexamethasone induced muscle atrophy and preventive effect of maca i lepidium meyenii i |
| topic | zebrafish muscle atrophy dexamethasone maca |
| url | https://www.mdpi.com/2073-4409/10/11/2879 |
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