Maize Bran Particle Size Governs the Community Composition and Metabolic Output of Human Gut Microbiota in in vitro Fermentations
Differences in the chemical and physical properties of dietary fibers are increasingly known to exert effects on their fermentation by gut microbiota. Here, we demonstrate that maize bran particle size fractions show metabolic output and microbial community differences similar to those we previously...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-05-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.01009/full |
| _version_ | 1819018741164277760 |
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| author | Riya D. Thakkar Yunus E. Tuncil Bruce R. Hamaker Stephen R. Lindemann |
| author_facet | Riya D. Thakkar Yunus E. Tuncil Bruce R. Hamaker Stephen R. Lindemann |
| author_sort | Riya D. Thakkar |
| collection | DOAJ |
| description | Differences in the chemical and physical properties of dietary fibers are increasingly known to exert effects on their fermentation by gut microbiota. Here, we demonstrate that maize bran particle size fractions show metabolic output and microbial community differences similar to those we previously observed for wheat brans. As for wheat brans, maize bran particles varied in starch and protein content and in sugar composition with respect to size. We fermented maize bran particles varying in size in vitro with human fecal microbiota as inocula, measuring their metabolic fate [i.e., short-chain fatty acids (SCFAs)] and resulting community structure (via 16S rRNA gene amplicon sequencing). Metabolically, acetate, propionate and butyrate productions were size-dependent. 16S rRNA sequencing revealed that the size-dependent SCFA production was linked to divergent microbial community structures, which exerted effects at fine taxonomic resolution (the genus and species level). These results further suggest that the physical properties of bran particles, such as size, are important variables governing microbial community compositional and metabolic responses. |
| first_indexed | 2024-12-21T03:24:14Z |
| format | Article |
| id | doaj.art-9da7e052815e4a0cb0f078966a8d8de2 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-21T03:24:14Z |
| publishDate | 2020-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-9da7e052815e4a0cb0f078966a8d8de22022-12-21T19:17:38ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-05-011110.3389/fmicb.2020.01009532762Maize Bran Particle Size Governs the Community Composition and Metabolic Output of Human Gut Microbiota in in vitro FermentationsRiya D. Thakkar0Yunus E. Tuncil1Bruce R. Hamaker2Stephen R. Lindemann3Whistler Center for Carbohydrate Research, Department of Food Science, Purdue University, West Lafayette, IN, United StatesFood Engineering Department, Ordu University, Ordu, TurkeyWhistler Center for Carbohydrate Research, Department of Food Science, Purdue University, West Lafayette, IN, United StatesWhistler Center for Carbohydrate Research, Department of Food Science, Purdue University, West Lafayette, IN, United StatesDifferences in the chemical and physical properties of dietary fibers are increasingly known to exert effects on their fermentation by gut microbiota. Here, we demonstrate that maize bran particle size fractions show metabolic output and microbial community differences similar to those we previously observed for wheat brans. As for wheat brans, maize bran particles varied in starch and protein content and in sugar composition with respect to size. We fermented maize bran particles varying in size in vitro with human fecal microbiota as inocula, measuring their metabolic fate [i.e., short-chain fatty acids (SCFAs)] and resulting community structure (via 16S rRNA gene amplicon sequencing). Metabolically, acetate, propionate and butyrate productions were size-dependent. 16S rRNA sequencing revealed that the size-dependent SCFA production was linked to divergent microbial community structures, which exerted effects at fine taxonomic resolution (the genus and species level). These results further suggest that the physical properties of bran particles, such as size, are important variables governing microbial community compositional and metabolic responses.https://www.frontiersin.org/article/10.3389/fmicb.2020.01009/fullmaize branparticle sizemillingupper gastrointestinal digestionshort-chain fatty acidsbutyrate |
| spellingShingle | Riya D. Thakkar Yunus E. Tuncil Bruce R. Hamaker Stephen R. Lindemann Maize Bran Particle Size Governs the Community Composition and Metabolic Output of Human Gut Microbiota in in vitro Fermentations Frontiers in Microbiology maize bran particle size milling upper gastrointestinal digestion short-chain fatty acids butyrate |
| title | Maize Bran Particle Size Governs the Community Composition and Metabolic Output of Human Gut Microbiota in in vitro Fermentations |
| title_full | Maize Bran Particle Size Governs the Community Composition and Metabolic Output of Human Gut Microbiota in in vitro Fermentations |
| title_fullStr | Maize Bran Particle Size Governs the Community Composition and Metabolic Output of Human Gut Microbiota in in vitro Fermentations |
| title_full_unstemmed | Maize Bran Particle Size Governs the Community Composition and Metabolic Output of Human Gut Microbiota in in vitro Fermentations |
| title_short | Maize Bran Particle Size Governs the Community Composition and Metabolic Output of Human Gut Microbiota in in vitro Fermentations |
| title_sort | maize bran particle size governs the community composition and metabolic output of human gut microbiota in in vitro fermentations |
| topic | maize bran particle size milling upper gastrointestinal digestion short-chain fatty acids butyrate |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.01009/full |
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