In Vitro Investigation of D- and Lenantiomer Synergistic Efects of Some Amino Acids
D-amino acids can arise from endogenous microbial flora, from ingestion with the diet or from spontaneous racemization of L-amino acids during ageing. In this work, the behavior of methionine, homocysteine and cysteine enantiomers was investigated in human serum in vitro during 0-72 h at incubation...
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| Format: | Article |
| Language: | English |
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SciCell s.r.o.
2016-06-01
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| Series: | Nova Biotechnologica et Chimica |
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| Online Access: | http://www.degruyter.com/view/j/nbec.2016.15.issue-1/nbec-2016-0005/nbec-2016-0005.xml?format=INT |
| _version_ | 1818533647405285376 |
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| author | Bystrická Zuzana Lehotay Jozef |
| author_facet | Bystrická Zuzana Lehotay Jozef |
| author_sort | Bystrická Zuzana |
| collection | DOAJ |
| description | D-amino acids can arise from endogenous microbial flora, from ingestion with the diet or from spontaneous racemization of L-amino acids during ageing. In this work, the behavior of methionine, homocysteine and cysteine enantiomers was investigated in human serum in vitro during 0-72 h at incubation temperature 37°C. The separation of enantiomers was realized in two dimensional on-line system (the connection of an achiral column Purospher RP-18 endcapped and a chiral column Chirobiotic TAG). This system allowed simultaneous monitoring all tested amino acids and their enantiomers. The possible effect D-enantiomer on the behavior of its L-enantiomer (the synergistic effect) was evaluated during incubation time. The first results have showed that no synergistic effect of D-enantiomer on its Lisomer has been observed in our experimental conditions in vitro. |
| first_indexed | 2024-12-11T18:01:29Z |
| format | Article |
| id | doaj.art-9da80a0ae1f241828ed1c8883ed25be1 |
| institution | Directory Open Access Journal |
| issn | 1338-6905 |
| language | English |
| last_indexed | 2024-12-11T18:01:29Z |
| publishDate | 2016-06-01 |
| publisher | SciCell s.r.o. |
| record_format | Article |
| series | Nova Biotechnologica et Chimica |
| spelling | doaj.art-9da80a0ae1f241828ed1c8883ed25be12022-12-22T00:55:53ZengSciCell s.r.o.Nova Biotechnologica et Chimica1338-69052016-06-01151475410.1515/nbec-2016-0005nbec-2016-0005In Vitro Investigation of D- and Lenantiomer Synergistic Efects of Some Amino AcidsBystrická Zuzana0Lehotay Jozef1Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova 2, Bratislava, SK-811 08, Slovak RepublicDepartment of Chemistry, University of SS. Cyril and Methodius, J. Herdu 2, Trnava, SK-917 01, Slovak RepublicD-amino acids can arise from endogenous microbial flora, from ingestion with the diet or from spontaneous racemization of L-amino acids during ageing. In this work, the behavior of methionine, homocysteine and cysteine enantiomers was investigated in human serum in vitro during 0-72 h at incubation temperature 37°C. The separation of enantiomers was realized in two dimensional on-line system (the connection of an achiral column Purospher RP-18 endcapped and a chiral column Chirobiotic TAG). This system allowed simultaneous monitoring all tested amino acids and their enantiomers. The possible effect D-enantiomer on the behavior of its L-enantiomer (the synergistic effect) was evaluated during incubation time. The first results have showed that no synergistic effect of D-enantiomer on its Lisomer has been observed in our experimental conditions in vitro.http://www.degruyter.com/view/j/nbec.2016.15.issue-1/nbec-2016-0005/nbec-2016-0005.xml?format=INTamino acidenantiomersserumsynergism |
| spellingShingle | Bystrická Zuzana Lehotay Jozef In Vitro Investigation of D- and Lenantiomer Synergistic Efects of Some Amino Acids Nova Biotechnologica et Chimica amino acid enantiomers serum synergism |
| title | In Vitro Investigation of D- and Lenantiomer Synergistic Efects of Some Amino Acids |
| title_full | In Vitro Investigation of D- and Lenantiomer Synergistic Efects of Some Amino Acids |
| title_fullStr | In Vitro Investigation of D- and Lenantiomer Synergistic Efects of Some Amino Acids |
| title_full_unstemmed | In Vitro Investigation of D- and Lenantiomer Synergistic Efects of Some Amino Acids |
| title_short | In Vitro Investigation of D- and Lenantiomer Synergistic Efects of Some Amino Acids |
| title_sort | in vitro investigation of d and lenantiomer synergistic efects of some amino acids |
| topic | amino acid enantiomers serum synergism |
| url | http://www.degruyter.com/view/j/nbec.2016.15.issue-1/nbec-2016-0005/nbec-2016-0005.xml?format=INT |
| work_keys_str_mv | AT bystrickazuzana invitroinvestigationofdandlenantiomersynergisticefectsofsomeaminoacids AT lehotayjozef invitroinvestigationofdandlenantiomersynergisticefectsofsomeaminoacids |