Biochemical Properties and Immunogenic Epitopes of Echinococcus granulosus Glutathione S-Transferase as a Vaccine Target: In-Silico Study
Background: The current in silico study was done to determine the primary biochemical features and immunogenic epitopes of Echinococcus granulosus glutathione S-transferase protein as a potential vaccine candidate. Methods: Several web tools were employed to predict physico-chemical properties,...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2024-03-01
|
| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3839 |
| _version_ | 1797266441203875840 |
|---|---|
| author | Sasan Khazaei Abdolhossein Dalimi Majid Pirestani Fatemeh Ghaffarifar |
| author_facet | Sasan Khazaei Abdolhossein Dalimi Majid Pirestani Fatemeh Ghaffarifar |
| author_sort | Sasan Khazaei |
| collection | DOAJ |
| description |
Background: The current in silico study was done to determine the primary biochemical features and immunogenic epitopes of Echinococcus granulosus glutathione S-transferase protein as a potential vaccine candidate.
Methods: Several web tools were employed to predict physico-chemical properties, antigenicity, allergenicity, solubility, post-translational modification (PTM) sites, subcellular localization, signal peptide, transmembrane domain, secondary and tertiary structure followed by refinement and validations. In addition, B-cell epitopes were predicted and were screened using various web servers, while MHC-binding and CTL epitopes were predicted using IEDB and NetCTL servers, respectively.
Results: The protein had 219 residues with a molecular weight of 25.55 kDa and alkaline isoelectric pH (7.5). This protein was stable, thermotolerant (aliphatic index: 78.04) and hydrophilic (GRAVY: -0.440). The predicted antigenicity scores were low and the protein was non-allergenic in nature. There were no transmembrane domain and signal peptide in the sequence. Moreover, several B-cell, MHC-binding and CTL epitopes were found in the EgGST protein, which could be further used in multi-epitope vaccines.
Conclusion: Further studies are needed on the development of vaccines in vivo using EgGST alone or in combination with other antigens in the future.
|
| first_indexed | 2024-04-25T01:00:44Z |
| format | Article |
| id | doaj.art-9daa085516834735af81e6b29d667ac7 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-04-25T01:00:44Z |
| publishDate | 2024-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-9daa085516834735af81e6b29d667ac72024-03-11T05:30:02ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2024-03-01191Biochemical Properties and Immunogenic Epitopes of Echinococcus granulosus Glutathione S-Transferase as a Vaccine Target: In-Silico StudySasan Khazaei0Abdolhossein Dalimi1Majid Pirestani2Fatemeh Ghaffarifar3Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Background: The current in silico study was done to determine the primary biochemical features and immunogenic epitopes of Echinococcus granulosus glutathione S-transferase protein as a potential vaccine candidate. Methods: Several web tools were employed to predict physico-chemical properties, antigenicity, allergenicity, solubility, post-translational modification (PTM) sites, subcellular localization, signal peptide, transmembrane domain, secondary and tertiary structure followed by refinement and validations. In addition, B-cell epitopes were predicted and were screened using various web servers, while MHC-binding and CTL epitopes were predicted using IEDB and NetCTL servers, respectively. Results: The protein had 219 residues with a molecular weight of 25.55 kDa and alkaline isoelectric pH (7.5). This protein was stable, thermotolerant (aliphatic index: 78.04) and hydrophilic (GRAVY: -0.440). The predicted antigenicity scores were low and the protein was non-allergenic in nature. There were no transmembrane domain and signal peptide in the sequence. Moreover, several B-cell, MHC-binding and CTL epitopes were found in the EgGST protein, which could be further used in multi-epitope vaccines. Conclusion: Further studies are needed on the development of vaccines in vivo using EgGST alone or in combination with other antigens in the future. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3839Echinococcus granulosusGlutathione S-transferaseBioinformaticsVaccine |
| spellingShingle | Sasan Khazaei Abdolhossein Dalimi Majid Pirestani Fatemeh Ghaffarifar Biochemical Properties and Immunogenic Epitopes of Echinococcus granulosus Glutathione S-Transferase as a Vaccine Target: In-Silico Study Iranian Journal of Parasitology Echinococcus granulosus Glutathione S-transferase Bioinformatics Vaccine |
| title | Biochemical Properties and Immunogenic Epitopes of Echinococcus granulosus Glutathione S-Transferase as a Vaccine Target: In-Silico Study |
| title_full | Biochemical Properties and Immunogenic Epitopes of Echinococcus granulosus Glutathione S-Transferase as a Vaccine Target: In-Silico Study |
| title_fullStr | Biochemical Properties and Immunogenic Epitopes of Echinococcus granulosus Glutathione S-Transferase as a Vaccine Target: In-Silico Study |
| title_full_unstemmed | Biochemical Properties and Immunogenic Epitopes of Echinococcus granulosus Glutathione S-Transferase as a Vaccine Target: In-Silico Study |
| title_short | Biochemical Properties and Immunogenic Epitopes of Echinococcus granulosus Glutathione S-Transferase as a Vaccine Target: In-Silico Study |
| title_sort | biochemical properties and immunogenic epitopes of echinococcus granulosus glutathione s transferase as a vaccine target in silico study |
| topic | Echinococcus granulosus Glutathione S-transferase Bioinformatics Vaccine |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3839 |
| work_keys_str_mv | AT sasankhazaei biochemicalpropertiesandimmunogenicepitopesofechinococcusgranulosusglutathionestransferaseasavaccinetargetinsilicostudy AT abdolhosseindalimi biochemicalpropertiesandimmunogenicepitopesofechinococcusgranulosusglutathionestransferaseasavaccinetargetinsilicostudy AT majidpirestani biochemicalpropertiesandimmunogenicepitopesofechinococcusgranulosusglutathionestransferaseasavaccinetargetinsilicostudy AT fatemehghaffarifar biochemicalpropertiesandimmunogenicepitopesofechinococcusgranulosusglutathionestransferaseasavaccinetargetinsilicostudy |