Transcriptome Analysis Reveals the Inducing Effect of <i>Bacillus siamensis</i> on Disease Resistance in Postharvest Mango Fruit
Postharvest anthracnose, caused by the fungus <i>Colletotrichum gloeosporioides</i>, is one of the most important postharvest diseases of mangoes worldwide. <i>Bacillus siamensis</i> (<i>B. siamensis</i>), as a biocontrol bacteria, has significant effects on inhib...
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MDPI AG
2022-01-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/1/107 |
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| author | Zecheng Jiang Rui Li Yue Tang Ziyu Cheng Minjie Qian Wen Li Yuanzhi Shao |
| author_facet | Zecheng Jiang Rui Li Yue Tang Ziyu Cheng Minjie Qian Wen Li Yuanzhi Shao |
| author_sort | Zecheng Jiang |
| collection | DOAJ |
| description | Postharvest anthracnose, caused by the fungus <i>Colletotrichum gloeosporioides</i>, is one of the most important postharvest diseases of mangoes worldwide. <i>Bacillus siamensis</i> (<i>B. siamensis</i>), as a biocontrol bacteria, has significant effects on inhibiting disease and improving the quality of fruits and vegetables. In this study, pre-storage application of <i>B. siamensis</i> significantly induced disease resistance and decreased disease index (DI) of stored mango fruit. To investigate the induction mechanisms of <i>B. siamensis</i>, comparative transcriptome analysis of mango fruit samples during the storage were established. In total, 234,808 unique transcripts were assembled and 56,704 differentially expressed genes (DEGs) were identified by comparative transcriptome analysis. Gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of DEGs showed that most of the DEGs involved in plant-pathogen interaction, plant hormone signal transduction, and biosynthesis of resistant substances were enriched. Fourteen DEGs related to disease-resistance were validated by qRT-PCR, which well corresponded to the FPKM value obtained from the transcriptome data. These results indicate that <i>B. siamensis</i> treatment may act to induce disease resistance of mango fruit by affecting multiple pathways. These findings not only reveal the transcriptional regulatory mechanisms that govern postharvest disease, but also develop a biological strategy to maintain quality of post-harvest mango fruit. |
| first_indexed | 2024-03-10T03:41:41Z |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T03:41:41Z |
| publishDate | 2022-01-01 |
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| series | Foods |
| spelling | doaj.art-9daa9e0aeb8a4eb0af51fbdda23461ba2023-11-23T11:31:33ZengMDPI AGFoods2304-81582022-01-0111110710.3390/foods11010107Transcriptome Analysis Reveals the Inducing Effect of <i>Bacillus siamensis</i> on Disease Resistance in Postharvest Mango FruitZecheng Jiang0Rui Li1Yue Tang2Ziyu Cheng3Minjie Qian4Wen Li5Yuanzhi Shao6College of Food Science and Engineering, Hainan University, Haikou 570228, ChinaKey Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, College of Horticulture, Hainan University, Haikou 570228, ChinaCollege of Food Science and Engineering, Hainan University, Haikou 570228, ChinaCollege of Food Science and Engineering, Hainan University, Haikou 570228, ChinaKey Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, College of Horticulture, Hainan University, Haikou 570228, ChinaKey Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, College of Horticulture, Hainan University, Haikou 570228, ChinaSchool of Life Sciences, Hainan University, Haikou 570228, ChinaPostharvest anthracnose, caused by the fungus <i>Colletotrichum gloeosporioides</i>, is one of the most important postharvest diseases of mangoes worldwide. <i>Bacillus siamensis</i> (<i>B. siamensis</i>), as a biocontrol bacteria, has significant effects on inhibiting disease and improving the quality of fruits and vegetables. In this study, pre-storage application of <i>B. siamensis</i> significantly induced disease resistance and decreased disease index (DI) of stored mango fruit. To investigate the induction mechanisms of <i>B. siamensis</i>, comparative transcriptome analysis of mango fruit samples during the storage were established. In total, 234,808 unique transcripts were assembled and 56,704 differentially expressed genes (DEGs) were identified by comparative transcriptome analysis. Gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of DEGs showed that most of the DEGs involved in plant-pathogen interaction, plant hormone signal transduction, and biosynthesis of resistant substances were enriched. Fourteen DEGs related to disease-resistance were validated by qRT-PCR, which well corresponded to the FPKM value obtained from the transcriptome data. These results indicate that <i>B. siamensis</i> treatment may act to induce disease resistance of mango fruit by affecting multiple pathways. These findings not only reveal the transcriptional regulatory mechanisms that govern postharvest disease, but also develop a biological strategy to maintain quality of post-harvest mango fruit.https://www.mdpi.com/2304-8158/11/1/107<i>Bacillus siamensis</i>mango fruitdisease resistancetranscriptome analysisgene expression |
| spellingShingle | Zecheng Jiang Rui Li Yue Tang Ziyu Cheng Minjie Qian Wen Li Yuanzhi Shao Transcriptome Analysis Reveals the Inducing Effect of <i>Bacillus siamensis</i> on Disease Resistance in Postharvest Mango Fruit Foods <i>Bacillus siamensis</i> mango fruit disease resistance transcriptome analysis gene expression |
| title | Transcriptome Analysis Reveals the Inducing Effect of <i>Bacillus siamensis</i> on Disease Resistance in Postharvest Mango Fruit |
| title_full | Transcriptome Analysis Reveals the Inducing Effect of <i>Bacillus siamensis</i> on Disease Resistance in Postharvest Mango Fruit |
| title_fullStr | Transcriptome Analysis Reveals the Inducing Effect of <i>Bacillus siamensis</i> on Disease Resistance in Postharvest Mango Fruit |
| title_full_unstemmed | Transcriptome Analysis Reveals the Inducing Effect of <i>Bacillus siamensis</i> on Disease Resistance in Postharvest Mango Fruit |
| title_short | Transcriptome Analysis Reveals the Inducing Effect of <i>Bacillus siamensis</i> on Disease Resistance in Postharvest Mango Fruit |
| title_sort | transcriptome analysis reveals the inducing effect of i bacillus siamensis i on disease resistance in postharvest mango fruit |
| topic | <i>Bacillus siamensis</i> mango fruit disease resistance transcriptome analysis gene expression |
| url | https://www.mdpi.com/2304-8158/11/1/107 |
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