Phytochemical Analysis and Antioxidant Activity of Eight Cultivars of Tea (Camellia sinensis) and Rapid Discrimination with FTIR Spectroscopy and Pattern Recognition Techniques
Background: Tea (Camellia sinensis L.O. Kuntze) is one of the most commonly consumed beverages globally, with several beneficial health effects. The composition of leaves is affected by different factors such as climate and tea cultivar. Methods: In this study, the total phenolic, flavonoid, and tan...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2023-01-01
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| Series: | Pharmaceutical Sciences |
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| Online Access: | https://ps.tbzmed.ac.ir/PDF/ps-29-100.pdf |
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| author | Fatemeh Yousefbeyk Heshmatollah Ebrahimi-Najafabadi Sara Dabirian Salar Salimi Farshid Baniardalani Fatemeh Azmian Moghadam Saeed Ghasemi |
| author_facet | Fatemeh Yousefbeyk Heshmatollah Ebrahimi-Najafabadi Sara Dabirian Salar Salimi Farshid Baniardalani Fatemeh Azmian Moghadam Saeed Ghasemi |
| author_sort | Fatemeh Yousefbeyk |
| collection | DOAJ |
| description | Background: Tea (Camellia sinensis L.O. Kuntze) is one of the most commonly consumed beverages globally, with several beneficial health effects. The composition of leaves is affected by different factors such as climate and tea cultivar. Methods: In this study, the total phenolic, flavonoid, and tannin contents, and antioxidant activities of eight cultivars of tea growing in Iran were determined. The epigallocatechin gallate (EGCG) contents were measured by high-performance liquid chromatography (HPLC) analysis. The Fourier-transform infrared (FT-IR) spectra were used to construct a supervised pattern recognition model using a genetic algorithm-support vector machine (GA-SVM) for the classification of cultivars. Results: The results indicated a wide variation of total phenolic content (178.04 to 316.09 mg gallic acid equivalents (GAE)/g extract). Also, the flavonoid contents ranged from 25.54 to 41.1 mg quercetin equivalents )QE(/g extract. All the samples had close tannin amounts (ranging from 40.32 to 45.90 mg as tannic acid equivalent (TAE)/g extract). There was a significant linear relationship among total phenolic and flavonoid contents and antioxidant properties. The cultivars DN, PK2, and C.Y.9 had the highest content of phenolic and flavonoid content as well as the best antioxidant activity. The EGCG contents were from 2.66 to 4.12%. The highest amount of EGCG was found in cultivars 282, PK2, and C.Y.9. The discriminative region of FT-IR spectra (1350-1650 cm-1) was selected using a GA-SVM. This model showed 100% sensitivity and specificity for training and test sets. Conclusion: The leaves’ phytochemical compositions and antioxidant effect are deeply dependent on the type of tea cultivars. The cultivars PK2 and C.Y.9 can be considered richer sources of polyphenols, especially EGCG. The eight different cultivars can be classified based on chemical components using the recorded FT-IR spectra. |
| first_indexed | 2024-04-09T17:50:38Z |
| format | Article |
| id | doaj.art-9dad83d350b44e0e9bc2d11bae8dccd7 |
| institution | Directory Open Access Journal |
| issn | 2383-2886 |
| language | English |
| last_indexed | 2024-04-09T17:50:38Z |
| publishDate | 2023-01-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Pharmaceutical Sciences |
| spelling | doaj.art-9dad83d350b44e0e9bc2d11bae8dccd72023-04-16T08:17:59ZengTabriz University of Medical SciencesPharmaceutical Sciences2383-28862023-01-0129110011010.34172/PS.2022.27ps-35960Phytochemical Analysis and Antioxidant Activity of Eight Cultivars of Tea (Camellia sinensis) and Rapid Discrimination with FTIR Spectroscopy and Pattern Recognition TechniquesFatemeh Yousefbeyk0Heshmatollah Ebrahimi-Najafabadi1Sara Dabirian2Salar Salimi3Farshid Baniardalani4Fatemeh Azmian Moghadam5Saeed Ghasemi6Department of Pharmacognosy, School of Pharmacy, Guilan University of Medical Sciences, Rasht, IranDepartment of Medicinal Chemistry, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran.Department of Pharmaceutical Biotechnology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran.Department of Pharmacognosy, School of Pharmacy, Guilan University of Medical Sciences, Rasht, IranDepartment of Pharmacognosy, School of Pharmacy, Guilan University of Medical Sciences, Rasht, IranDepartment of Medicinal Chemistry, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran.Department of Medicinal Chemistry, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran.Background: Tea (Camellia sinensis L.O. Kuntze) is one of the most commonly consumed beverages globally, with several beneficial health effects. The composition of leaves is affected by different factors such as climate and tea cultivar. Methods: In this study, the total phenolic, flavonoid, and tannin contents, and antioxidant activities of eight cultivars of tea growing in Iran were determined. The epigallocatechin gallate (EGCG) contents were measured by high-performance liquid chromatography (HPLC) analysis. The Fourier-transform infrared (FT-IR) spectra were used to construct a supervised pattern recognition model using a genetic algorithm-support vector machine (GA-SVM) for the classification of cultivars. Results: The results indicated a wide variation of total phenolic content (178.04 to 316.09 mg gallic acid equivalents (GAE)/g extract). Also, the flavonoid contents ranged from 25.54 to 41.1 mg quercetin equivalents )QE(/g extract. All the samples had close tannin amounts (ranging from 40.32 to 45.90 mg as tannic acid equivalent (TAE)/g extract). There was a significant linear relationship among total phenolic and flavonoid contents and antioxidant properties. The cultivars DN, PK2, and C.Y.9 had the highest content of phenolic and flavonoid content as well as the best antioxidant activity. The EGCG contents were from 2.66 to 4.12%. The highest amount of EGCG was found in cultivars 282, PK2, and C.Y.9. The discriminative region of FT-IR spectra (1350-1650 cm-1) was selected using a GA-SVM. This model showed 100% sensitivity and specificity for training and test sets. Conclusion: The leaves’ phytochemical compositions and antioxidant effect are deeply dependent on the type of tea cultivars. The cultivars PK2 and C.Y.9 can be considered richer sources of polyphenols, especially EGCG. The eight different cultivars can be classified based on chemical components using the recorded FT-IR spectra.https://ps.tbzmed.ac.ir/PDF/ps-29-100.pdfantioxidantegcgcamellia sinensispattern recognitionphenolic compound |
| spellingShingle | Fatemeh Yousefbeyk Heshmatollah Ebrahimi-Najafabadi Sara Dabirian Salar Salimi Farshid Baniardalani Fatemeh Azmian Moghadam Saeed Ghasemi Phytochemical Analysis and Antioxidant Activity of Eight Cultivars of Tea (Camellia sinensis) and Rapid Discrimination with FTIR Spectroscopy and Pattern Recognition Techniques Pharmaceutical Sciences antioxidant egcg camellia sinensis pattern recognition phenolic compound |
| title | Phytochemical Analysis and Antioxidant Activity of Eight Cultivars of Tea (Camellia sinensis) and Rapid Discrimination with FTIR Spectroscopy and Pattern Recognition Techniques |
| title_full | Phytochemical Analysis and Antioxidant Activity of Eight Cultivars of Tea (Camellia sinensis) and Rapid Discrimination with FTIR Spectroscopy and Pattern Recognition Techniques |
| title_fullStr | Phytochemical Analysis and Antioxidant Activity of Eight Cultivars of Tea (Camellia sinensis) and Rapid Discrimination with FTIR Spectroscopy and Pattern Recognition Techniques |
| title_full_unstemmed | Phytochemical Analysis and Antioxidant Activity of Eight Cultivars of Tea (Camellia sinensis) and Rapid Discrimination with FTIR Spectroscopy and Pattern Recognition Techniques |
| title_short | Phytochemical Analysis and Antioxidant Activity of Eight Cultivars of Tea (Camellia sinensis) and Rapid Discrimination with FTIR Spectroscopy and Pattern Recognition Techniques |
| title_sort | phytochemical analysis and antioxidant activity of eight cultivars of tea camellia sinensis and rapid discrimination with ftir spectroscopy and pattern recognition techniques |
| topic | antioxidant egcg camellia sinensis pattern recognition phenolic compound |
| url | https://ps.tbzmed.ac.ir/PDF/ps-29-100.pdf |
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