Effect of dietary bile acids: Growth performance, immune response, genes expression of fatty acid metabolism, intestinal, and liver morphology of striped catfish (Pangasianodon hypophthalmus)

Seven isonitrogenous (31.70% protein) and isolipidic (5.20%) experimental diets were made by supplementing a control diet (BA0) with 0.25, 0.50, 0.75, 1.0, 1.25, and 1.5 g bile acid (BA) kg−1 diet, respectively. The diets were fed to striped catfish (Pangasianodon hypophthalmus) to monitor the effec...

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Main Authors: Ali Hussein Adam, Marc Verdegem, Ali A. Soliman, Mohamed Zaki, Riad H. Khalil, Abd-Elaziz M. Nour, Asmaa A. Khaled, Mohammed F. El Basuini, Hala Saber Khalil
Format: Article
Language:English
Published: Elsevier 2023-04-01
Series:Aquaculture Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2352513423000492
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author Ali Hussein Adam
Marc Verdegem
Ali A. Soliman
Mohamed Zaki
Riad H. Khalil
Abd-Elaziz M. Nour
Asmaa A. Khaled
Mohammed F. El Basuini
Hala Saber Khalil
author_facet Ali Hussein Adam
Marc Verdegem
Ali A. Soliman
Mohamed Zaki
Riad H. Khalil
Abd-Elaziz M. Nour
Asmaa A. Khaled
Mohammed F. El Basuini
Hala Saber Khalil
author_sort Ali Hussein Adam
collection DOAJ
description Seven isonitrogenous (31.70% protein) and isolipidic (5.20%) experimental diets were made by supplementing a control diet (BA0) with 0.25, 0.50, 0.75, 1.0, 1.25, and 1.5 g bile acid (BA) kg−1 diet, respectively. The diets were fed to striped catfish (Pangasianodon hypophthalmus) to monitor the effects on the growth, non-specific immune response, gene expression of fatty acids metabolism, liver, and their intestinal morphology. The average initial fish weight was 10.3 ± 0.20 (SD) g. The diets were randomly assigned to 21 hapas, each stocked with 10 fish and fed for 70 days. Supplementing BA1.0 and BA1.25 significantly improved growth and nutrient utilization (P < 0.05) compared to BA0 and other treatments. Additionally, the hematological analyses red blood cell count (RBC), haemoglobin, packed cell volume (PCV) and white blood cell count (WBC) amplified in the subsequent order: BA1.0, BA1.25, BA0.75, BA1.50, and BA0.50 g kg−1 diet and finally BA0.25 g kg−1 diet compared to the control diet. The rates of serum lysozyme activity, biochemical parameters, phagocytic index, phagocytic activity, and antioxidants parameters were considerably improved (P ≤ 0.05) in fish fed diets BA1.0 and BA1.25. In the intestinal measurement and liver histomorphometricall, the highest values were observed on BA1.0 and BA1.25. Among the five genes assayed in the liver tissues, peritoneal fat, and musculature levels of ATGL, HSL, and CPT-1, genes expression increased in fish fed diets with increasing levels of bile acid added to the diet while, the expression of PPARα and FAS decreased. Consequently, bile acids are recommended at levels of 1.0–1.25 g kg−1 diet to improve the growth performance and immune status of striped catfish.
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spelling doaj.art-9daf344312cb416cb9bfa3f48a11f8032023-03-23T04:36:27ZengElsevierAquaculture Reports2352-51342023-04-0129101510Effect of dietary bile acids: Growth performance, immune response, genes expression of fatty acid metabolism, intestinal, and liver morphology of striped catfish (Pangasianodon hypophthalmus)Ali Hussein Adam0Marc Verdegem1Ali A. Soliman2Mohamed Zaki3Riad H. Khalil4Abd-Elaziz M. Nour5Asmaa A. Khaled6Mohammed F. El Basuini7Hala Saber Khalil8Animal Production Department, Faculty of Agriculture, Alexandria University, Alexandria, EgyptWageningen Institute of Animal Sciences, Aquaculture and Fisheries Group, Wageningen University and Research, Wageningen, the Netherlands; Corresponding author.National Institute of Oceanography and Fisheries (NIOF), EgyptAnimal Production Department, Faculty of Agriculture, Alexandria University, Alexandria, EgyptDepartment of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Alexandria University, Alexandria 22758, EgyptAnimal Production Department, Faculty of Agriculture, Alexandria University, Alexandria, EgyptAnimal and Fish Production Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria, EgyptAnimal Production Department, Faculty of Agriculture, Tanta University, 31527 Tanta, Egypt; Faculty of Desert Agriculture, King Salman International University, South Sinai 46618, EgyptWageningen Institute of Animal Sciences, Aquaculture and Fisheries Group, Wageningen University and Research, Wageningen, the Netherlands; National Institute of Oceanography and Fisheries (NIOF), Egypt; Correspondence to: Wageningen University and Research, the Netherlands.Seven isonitrogenous (31.70% protein) and isolipidic (5.20%) experimental diets were made by supplementing a control diet (BA0) with 0.25, 0.50, 0.75, 1.0, 1.25, and 1.5 g bile acid (BA) kg−1 diet, respectively. The diets were fed to striped catfish (Pangasianodon hypophthalmus) to monitor the effects on the growth, non-specific immune response, gene expression of fatty acids metabolism, liver, and their intestinal morphology. The average initial fish weight was 10.3 ± 0.20 (SD) g. The diets were randomly assigned to 21 hapas, each stocked with 10 fish and fed for 70 days. Supplementing BA1.0 and BA1.25 significantly improved growth and nutrient utilization (P < 0.05) compared to BA0 and other treatments. Additionally, the hematological analyses red blood cell count (RBC), haemoglobin, packed cell volume (PCV) and white blood cell count (WBC) amplified in the subsequent order: BA1.0, BA1.25, BA0.75, BA1.50, and BA0.50 g kg−1 diet and finally BA0.25 g kg−1 diet compared to the control diet. The rates of serum lysozyme activity, biochemical parameters, phagocytic index, phagocytic activity, and antioxidants parameters were considerably improved (P ≤ 0.05) in fish fed diets BA1.0 and BA1.25. In the intestinal measurement and liver histomorphometricall, the highest values were observed on BA1.0 and BA1.25. Among the five genes assayed in the liver tissues, peritoneal fat, and musculature levels of ATGL, HSL, and CPT-1, genes expression increased in fish fed diets with increasing levels of bile acid added to the diet while, the expression of PPARα and FAS decreased. Consequently, bile acids are recommended at levels of 1.0–1.25 g kg−1 diet to improve the growth performance and immune status of striped catfish.http://www.sciencedirect.com/science/article/pii/S2352513423000492Feed utilizationBody compositionPhagocytic indexPeritoneal fatLiver histomorphometrically
spellingShingle Ali Hussein Adam
Marc Verdegem
Ali A. Soliman
Mohamed Zaki
Riad H. Khalil
Abd-Elaziz M. Nour
Asmaa A. Khaled
Mohammed F. El Basuini
Hala Saber Khalil
Effect of dietary bile acids: Growth performance, immune response, genes expression of fatty acid metabolism, intestinal, and liver morphology of striped catfish (Pangasianodon hypophthalmus)
Aquaculture Reports
Feed utilization
Body composition
Phagocytic index
Peritoneal fat
Liver histomorphometrically
title Effect of dietary bile acids: Growth performance, immune response, genes expression of fatty acid metabolism, intestinal, and liver morphology of striped catfish (Pangasianodon hypophthalmus)
title_full Effect of dietary bile acids: Growth performance, immune response, genes expression of fatty acid metabolism, intestinal, and liver morphology of striped catfish (Pangasianodon hypophthalmus)
title_fullStr Effect of dietary bile acids: Growth performance, immune response, genes expression of fatty acid metabolism, intestinal, and liver morphology of striped catfish (Pangasianodon hypophthalmus)
title_full_unstemmed Effect of dietary bile acids: Growth performance, immune response, genes expression of fatty acid metabolism, intestinal, and liver morphology of striped catfish (Pangasianodon hypophthalmus)
title_short Effect of dietary bile acids: Growth performance, immune response, genes expression of fatty acid metabolism, intestinal, and liver morphology of striped catfish (Pangasianodon hypophthalmus)
title_sort effect of dietary bile acids growth performance immune response genes expression of fatty acid metabolism intestinal and liver morphology of striped catfish pangasianodon hypophthalmus
topic Feed utilization
Body composition
Phagocytic index
Peritoneal fat
Liver histomorphometrically
url http://www.sciencedirect.com/science/article/pii/S2352513423000492
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