The MYB-CC Transcription Factor PHOSPHATE STARVATION RESPONSE-LIKE 7 (PHL7) Functions in Phosphate Homeostasis and Affects Salt Stress Tolerance in Rice
Inorganic phosphate (Pi) homeostasis plays an important role in plant growth and abiotic stress tolerance. Several MYB-CC transcription factors involved in Pi homeostasis have been identified in rice (<i>Oryza sativa</i>). PHOSPHATE STARVATION RESPONSE-LIKE 7 (PHL7) is a class II MYC-CC...
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MDPI AG
2024-02-01
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| Online Access: | https://www.mdpi.com/2223-7747/13/5/637 |
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| author | Won Tae Yang Ki Deuk Bae Seon-Woo Lee Ki Hong Jung Sunok Moon Prakash Basnet Ik-Young Choi Taeyoung Um Doh Hoon Kim |
| author_facet | Won Tae Yang Ki Deuk Bae Seon-Woo Lee Ki Hong Jung Sunok Moon Prakash Basnet Ik-Young Choi Taeyoung Um Doh Hoon Kim |
| author_sort | Won Tae Yang |
| collection | DOAJ |
| description | Inorganic phosphate (Pi) homeostasis plays an important role in plant growth and abiotic stress tolerance. Several MYB-CC transcription factors involved in Pi homeostasis have been identified in rice (<i>Oryza sativa</i>). PHOSPHATE STARVATION RESPONSE-LIKE 7 (PHL7) is a class II MYC-CC protein, in which the MYC-CC domain is located at the N terminus. In this study, we established that OsPHL7 is localized to the nucleus and that the encoding gene is induced by Pi deficiency. The Pi-responsive genes and Pi transporter genes are positively regulated by OsPHL7. The overexpression of <i>OsPHL7</i> enhanced the tolerance of rice plants to Pi starvation, whereas the RNA interference-based knockdown of this gene resulted in increased sensitivity to Pi deficiency. Transgenic rice plants overexpressing <i>OsPHL7</i> produced more roots than wild-type plants under both Pi-sufficient and Pi-deficient conditions and accumulated more Pi in the shoots and roots. In addition, the overexpression of <i>OsPHL7</i> enhanced rice tolerance to salt stress. Together, these results demonstrate that <i>OsPHL7</i> is involved in the maintenance of Pi homeostasis and enhances tolerance to Pi deficiency and salt stress in rice. |
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| language | English |
| last_indexed | 2024-04-25T00:22:21Z |
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| spelling | doaj.art-9db13f6a80564c54adc1c1663428d0312024-03-12T16:52:44ZengMDPI AGPlants2223-77472024-02-0113563710.3390/plants13050637The MYB-CC Transcription Factor PHOSPHATE STARVATION RESPONSE-LIKE 7 (PHL7) Functions in Phosphate Homeostasis and Affects Salt Stress Tolerance in RiceWon Tae Yang0Ki Deuk Bae1Seon-Woo Lee2Ki Hong Jung3Sunok Moon4Prakash Basnet5Ik-Young Choi6Taeyoung Um7Doh Hoon Kim8College of Life Science and Natural Resources, Dong-A University, Busan 49315, Republic of KoreaCollege of Life Science and Natural Resources, Dong-A University, Busan 49315, Republic of KoreaCollege of Life Science and Natural Resources, Dong-A University, Busan 49315, Republic of KoreaGraduate School of Green-Bio Science, Kyung Hee University, Yongin 17104, Republic of KoreaGraduate School of Green-Bio Science, Kyung Hee University, Yongin 17104, Republic of KoreaDepartment of Agriculture and Life Industry, Kangwon National University, Chuncheon 24341, Republic of KoreaDepartment of Agriculture and Life Industry, Kangwon National University, Chuncheon 24341, Republic of KoreaDepartment of Agriculture and Life Science Institute, Kangwon National University, Chuncheon 24341, Republic of KoreaCollege of Life Science and Natural Resources, Dong-A University, Busan 49315, Republic of KoreaInorganic phosphate (Pi) homeostasis plays an important role in plant growth and abiotic stress tolerance. Several MYB-CC transcription factors involved in Pi homeostasis have been identified in rice (<i>Oryza sativa</i>). PHOSPHATE STARVATION RESPONSE-LIKE 7 (PHL7) is a class II MYC-CC protein, in which the MYC-CC domain is located at the N terminus. In this study, we established that OsPHL7 is localized to the nucleus and that the encoding gene is induced by Pi deficiency. The Pi-responsive genes and Pi transporter genes are positively regulated by OsPHL7. The overexpression of <i>OsPHL7</i> enhanced the tolerance of rice plants to Pi starvation, whereas the RNA interference-based knockdown of this gene resulted in increased sensitivity to Pi deficiency. Transgenic rice plants overexpressing <i>OsPHL7</i> produced more roots than wild-type plants under both Pi-sufficient and Pi-deficient conditions and accumulated more Pi in the shoots and roots. In addition, the overexpression of <i>OsPHL7</i> enhanced rice tolerance to salt stress. Together, these results demonstrate that <i>OsPHL7</i> is involved in the maintenance of Pi homeostasis and enhances tolerance to Pi deficiency and salt stress in rice.https://www.mdpi.com/2223-7747/13/5/637MYB transcription factor<i>Oryza sativa</i>phosphorus homeostasisPi starvation responsesalt stress tolerance |
| spellingShingle | Won Tae Yang Ki Deuk Bae Seon-Woo Lee Ki Hong Jung Sunok Moon Prakash Basnet Ik-Young Choi Taeyoung Um Doh Hoon Kim The MYB-CC Transcription Factor PHOSPHATE STARVATION RESPONSE-LIKE 7 (PHL7) Functions in Phosphate Homeostasis and Affects Salt Stress Tolerance in Rice Plants MYB transcription factor <i>Oryza sativa</i> phosphorus homeostasis Pi starvation response salt stress tolerance |
| title | The MYB-CC Transcription Factor PHOSPHATE STARVATION RESPONSE-LIKE 7 (PHL7) Functions in Phosphate Homeostasis and Affects Salt Stress Tolerance in Rice |
| title_full | The MYB-CC Transcription Factor PHOSPHATE STARVATION RESPONSE-LIKE 7 (PHL7) Functions in Phosphate Homeostasis and Affects Salt Stress Tolerance in Rice |
| title_fullStr | The MYB-CC Transcription Factor PHOSPHATE STARVATION RESPONSE-LIKE 7 (PHL7) Functions in Phosphate Homeostasis and Affects Salt Stress Tolerance in Rice |
| title_full_unstemmed | The MYB-CC Transcription Factor PHOSPHATE STARVATION RESPONSE-LIKE 7 (PHL7) Functions in Phosphate Homeostasis and Affects Salt Stress Tolerance in Rice |
| title_short | The MYB-CC Transcription Factor PHOSPHATE STARVATION RESPONSE-LIKE 7 (PHL7) Functions in Phosphate Homeostasis and Affects Salt Stress Tolerance in Rice |
| title_sort | myb cc transcription factor phosphate starvation response like 7 phl7 functions in phosphate homeostasis and affects salt stress tolerance in rice |
| topic | MYB transcription factor <i>Oryza sativa</i> phosphorus homeostasis Pi starvation response salt stress tolerance |
| url | https://www.mdpi.com/2223-7747/13/5/637 |
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