Enzymatic Assessment of the State of Oil-Contaminated Soils in the South of Russia after Bioremediation
Soil pollution with oil as a result of accidents at oil pipelines and oil refineries is a frequent occurrence in the south of Russia. To restore such polluted lands, it is necessary to carry out soil remediation measures. This work aimed to evaluate the use of ameliorants of various natures (biochar...
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MDPI AG
2023-04-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/11/4/355 |
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| author | Tatyana Minnikova Sergey Kolesnikov Sofia Revina Anna Ruseva Vladimir Gaivoronsky |
| author_facet | Tatyana Minnikova Sergey Kolesnikov Sofia Revina Anna Ruseva Vladimir Gaivoronsky |
| author_sort | Tatyana Minnikova |
| collection | DOAJ |
| description | Soil pollution with oil as a result of accidents at oil pipelines and oil refineries is a frequent occurrence in the south of Russia. To restore such polluted lands, it is necessary to carry out soil remediation measures. This work aimed to evaluate the use of ameliorants of various natures (biochar, sodium humate, and microbial preparation Baikal EM-1) to restore the ecological state of oil-contaminated soils with different properties (Haplic Chernozem, Haplic Arenosols, Haplic Cambisols). To assess the ecological state of soils, the following physicochemical and biological indicators were studied: residual oil content, redox potential, and medium reaction (pH). Changes in enzymatic activity were also studied, including catalase, dehydrogenases, invertase, urease, and phosphatase. The greatest decomposition of oil in Haplic Chernozem and Haplic Cambisols was provided by Baikal EM-1 (56 and 26%), and in Haplic Arenosols, this was provided by biochar (94%) and sodium humate (93%). In oil-contaminated Haplic Cambisols, the content of easily soluble salts with the addition of biochar and Baikal EM-1 increased by 83 and 58%, respectively. The introduction of biochar caused an increase in pH from 5.3 (Haplic Cambisols) to 8.2 (Haplic Arenosols). The introduction of oil-contaminated Haplic Arenosols of biochar, humate, and Baikal stimulated the activity of catalase and dehydrogenases by 52–245%. The activity of invertase was stimulated in the Haplic Chernozem after the introduction of ameliorants by 15–50%. The activity of urease was stimulated after the introduction of ameliorants into borax and Arenosol by 15–250%. The most effective ameliorant for restoring the ecological state of Haplic Cambisols after oil pollution was biochar. For Haplic Arenosols, this was sodium humate, and for Haplic Chernozem, the effectiveness of biochar and sodium humate did not differ. The most informative indicator for the remediation of Haplic Chernozem and Haplic Cambisols was the activity of dehydrogenases, and for Haplic Arenosols, this was the activity of phosphatase. The results of the study should be used to biomonitor the ecological state of oil-contaminated soils after bioremediation. |
| first_indexed | 2024-03-11T04:28:18Z |
| format | Article |
| id | doaj.art-9db41fda551942aa86ea8c7923dde994 |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-11T04:28:18Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj.art-9db41fda551942aa86ea8c7923dde9942023-11-17T21:37:43ZengMDPI AGToxics2305-63042023-04-0111435510.3390/toxics11040355Enzymatic Assessment of the State of Oil-Contaminated Soils in the South of Russia after BioremediationTatyana Minnikova0Sergey Kolesnikov1Sofia Revina2Anna Ruseva3Vladimir Gaivoronsky4Department of Ecology and Nature Management, Academy of Biology and Biotechnology by D.I. Ivanovsky, Southern Federal University, Stachki Ave., 194/1, Rostov Region, 344090 Rostov-on-Don, RussiaDepartment of Ecology and Nature Management, Academy of Biology and Biotechnology by D.I. Ivanovsky, Southern Federal University, Stachki Ave., 194/1, Rostov Region, 344090 Rostov-on-Don, RussiaDepartment of Ecology and Nature Management, Academy of Biology and Biotechnology by D.I. Ivanovsky, Southern Federal University, Stachki Ave., 194/1, Rostov Region, 344090 Rostov-on-Don, RussiaDepartment of Ecology and Nature Management, Academy of Biology and Biotechnology by D.I. Ivanovsky, Southern Federal University, Stachki Ave., 194/1, Rostov Region, 344090 Rostov-on-Don, RussiaAcademy of Physical Culture and Sports, Department of Theoretical Foundations of Physical Education, Southern Federal University, St. Zorge, 5, Rostov Region, 344015 Rostov-on-Don, RussiaSoil pollution with oil as a result of accidents at oil pipelines and oil refineries is a frequent occurrence in the south of Russia. To restore such polluted lands, it is necessary to carry out soil remediation measures. This work aimed to evaluate the use of ameliorants of various natures (biochar, sodium humate, and microbial preparation Baikal EM-1) to restore the ecological state of oil-contaminated soils with different properties (Haplic Chernozem, Haplic Arenosols, Haplic Cambisols). To assess the ecological state of soils, the following physicochemical and biological indicators were studied: residual oil content, redox potential, and medium reaction (pH). Changes in enzymatic activity were also studied, including catalase, dehydrogenases, invertase, urease, and phosphatase. The greatest decomposition of oil in Haplic Chernozem and Haplic Cambisols was provided by Baikal EM-1 (56 and 26%), and in Haplic Arenosols, this was provided by biochar (94%) and sodium humate (93%). In oil-contaminated Haplic Cambisols, the content of easily soluble salts with the addition of biochar and Baikal EM-1 increased by 83 and 58%, respectively. The introduction of biochar caused an increase in pH from 5.3 (Haplic Cambisols) to 8.2 (Haplic Arenosols). The introduction of oil-contaminated Haplic Arenosols of biochar, humate, and Baikal stimulated the activity of catalase and dehydrogenases by 52–245%. The activity of invertase was stimulated in the Haplic Chernozem after the introduction of ameliorants by 15–50%. The activity of urease was stimulated after the introduction of ameliorants into borax and Arenosol by 15–250%. The most effective ameliorant for restoring the ecological state of Haplic Cambisols after oil pollution was biochar. For Haplic Arenosols, this was sodium humate, and for Haplic Chernozem, the effectiveness of biochar and sodium humate did not differ. The most informative indicator for the remediation of Haplic Chernozem and Haplic Cambisols was the activity of dehydrogenases, and for Haplic Arenosols, this was the activity of phosphatase. The results of the study should be used to biomonitor the ecological state of oil-contaminated soils after bioremediation.https://www.mdpi.com/2305-6304/11/4/355soilpollutionbiodiagnosticsactivity of catalaseactivity of dehydrogenasesactivity of invertase |
| spellingShingle | Tatyana Minnikova Sergey Kolesnikov Sofia Revina Anna Ruseva Vladimir Gaivoronsky Enzymatic Assessment of the State of Oil-Contaminated Soils in the South of Russia after Bioremediation Toxics soil pollution biodiagnostics activity of catalase activity of dehydrogenases activity of invertase |
| title | Enzymatic Assessment of the State of Oil-Contaminated Soils in the South of Russia after Bioremediation |
| title_full | Enzymatic Assessment of the State of Oil-Contaminated Soils in the South of Russia after Bioremediation |
| title_fullStr | Enzymatic Assessment of the State of Oil-Contaminated Soils in the South of Russia after Bioremediation |
| title_full_unstemmed | Enzymatic Assessment of the State of Oil-Contaminated Soils in the South of Russia after Bioremediation |
| title_short | Enzymatic Assessment of the State of Oil-Contaminated Soils in the South of Russia after Bioremediation |
| title_sort | enzymatic assessment of the state of oil contaminated soils in the south of russia after bioremediation |
| topic | soil pollution biodiagnostics activity of catalase activity of dehydrogenases activity of invertase |
| url | https://www.mdpi.com/2305-6304/11/4/355 |
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