Stem Cell Mesenchymal Injection Increases Platelet-Derived Growth Factors Level and Percentage of Collagen in Third-Degree Burn injured Mice
Introduction: Mesenchymal stem cell (MSC) has been demonstrated to accelerate wound healing. Platelet-derived growth factor (PDGF) and collagen have an important role in wound healing. However, the effect of MSC on levels of PDGF and number of collagen has not been established. Objectives: To determ...
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| Format: | Article |
| Language: | English |
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Universitas Sultan Agung Semarang
2017-03-01
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| Series: | Sains Medika |
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| Online Access: | http://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/1173 |
| _version_ | 1811283835926609920 |
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| author | Erna Ridawati Taufiqurrachman Nasihun Wiratno Wiratno |
| author_facet | Erna Ridawati Taufiqurrachman Nasihun Wiratno Wiratno |
| author_sort | Erna Ridawati |
| collection | DOAJ |
| description | Introduction: Mesenchymal stem cell (MSC) has been demonstrated to accelerate wound healing. Platelet-derived growth factor (PDGF) and collagen have an important role in wound healing. However, the effect of MSC on levels of PDGF and number of collagen has not been established. Objectives: To determine the effect of MSC on the levels of PDGF and the percentage of collagen in the burns healing.
Methods: in this experimental study, 20 BALB /c mice were divided into 4 groups: a control group (G-0) injected with 2 ml of distilled water and group 1 (G-1), Group 2 (G-2), and group 3 (G- 3) injected with MSC at the dose of 1 x 104, 2 x 104, and 4 x 104 respectively. The burn wound was made by attaching a metal nail that had been heated for 20 seconds at the feet of mice. Levels of PDGF on day 2 (PDGF-2) and 7 (PDGF-7) was assessed by ELISA, while on day 10 excision of skin was done to calculate the percentage of collagen.
Results: There was a significant difference in the levels of PDGF-2, PDGF-7, and the percentage of collagen ( P <0.05). Posthoc analysis showed that the levels of PDGF-2, PDGF-7, and percentage of collagen in the G-2 and G-3 was significantly higher than that of G-0, p <0.05. While the levels of PDGF-2, PDGF-7, and the percentage of collagen in the G-3 was significantly higher than that of G-2 and G-1 (p <0.05).
Conclusion: Injection of MSC at a dose of 2 x 1044 and 4 x 1044 increases the levels of PDGF and the percentage of collagen. |
| first_indexed | 2024-04-13T02:19:22Z |
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| id | doaj.art-9db613248f6e4be49df4c492c7c9d4d2 |
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| issn | 2085-1545 2339-093X |
| language | English |
| last_indexed | 2024-04-13T02:19:22Z |
| publishDate | 2017-03-01 |
| publisher | Universitas Sultan Agung Semarang |
| record_format | Article |
| series | Sains Medika |
| spelling | doaj.art-9db613248f6e4be49df4c492c7c9d4d22022-12-22T03:07:03ZengUniversitas Sultan Agung SemarangSains Medika2085-15452339-093X2017-03-0172495310.26532/sainsmed.v7i2.11731039Stem Cell Mesenchymal Injection Increases Platelet-Derived Growth Factors Level and Percentage of Collagen in Third-Degree Burn injured MiceErna Ridawati0Taufiqurrachman Nasihun1Wiratno Wiratno2Biomedical Sciences Department of the Medical Faculty of Sultan Agung Islamic University Jl. Kaligawe KM 4 Semarang, Central Java Indonesia, Telp. + 628 122 920 906 *Corresponding author : Email: ernarida93@yahoo.co.idBiomedical Sciences Department of the Medical Faculty of Sultan Agung Islamic University Jl. Kaligawe KM 4 Semarang, Central Java Indonesia, Telp. + 628 122 920 906Biomedical Sciences Department of the Medical Faculty of Sultan Agung Islamic University Jl. Kaligawe KM 4 Semarang, Central Java Indonesia, Telp. + 628 122 920 906Introduction: Mesenchymal stem cell (MSC) has been demonstrated to accelerate wound healing. Platelet-derived growth factor (PDGF) and collagen have an important role in wound healing. However, the effect of MSC on levels of PDGF and number of collagen has not been established. Objectives: To determine the effect of MSC on the levels of PDGF and the percentage of collagen in the burns healing. Methods: in this experimental study, 20 BALB /c mice were divided into 4 groups: a control group (G-0) injected with 2 ml of distilled water and group 1 (G-1), Group 2 (G-2), and group 3 (G- 3) injected with MSC at the dose of 1 x 104, 2 x 104, and 4 x 104 respectively. The burn wound was made by attaching a metal nail that had been heated for 20 seconds at the feet of mice. Levels of PDGF on day 2 (PDGF-2) and 7 (PDGF-7) was assessed by ELISA, while on day 10 excision of skin was done to calculate the percentage of collagen. Results: There was a significant difference in the levels of PDGF-2, PDGF-7, and the percentage of collagen ( P <0.05). Posthoc analysis showed that the levels of PDGF-2, PDGF-7, and percentage of collagen in the G-2 and G-3 was significantly higher than that of G-0, p <0.05. While the levels of PDGF-2, PDGF-7, and the percentage of collagen in the G-3 was significantly higher than that of G-2 and G-1 (p <0.05). Conclusion: Injection of MSC at a dose of 2 x 1044 and 4 x 1044 increases the levels of PDGF and the percentage of collagen.http://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/1173Mesenchymal stem cellsPDGFCollagen. |
| spellingShingle | Erna Ridawati Taufiqurrachman Nasihun Wiratno Wiratno Stem Cell Mesenchymal Injection Increases Platelet-Derived Growth Factors Level and Percentage of Collagen in Third-Degree Burn injured Mice Sains Medika Mesenchymal stem cells PDGF Collagen. |
| title | Stem Cell Mesenchymal Injection Increases Platelet-Derived Growth Factors Level and Percentage of Collagen in Third-Degree Burn injured Mice |
| title_full | Stem Cell Mesenchymal Injection Increases Platelet-Derived Growth Factors Level and Percentage of Collagen in Third-Degree Burn injured Mice |
| title_fullStr | Stem Cell Mesenchymal Injection Increases Platelet-Derived Growth Factors Level and Percentage of Collagen in Third-Degree Burn injured Mice |
| title_full_unstemmed | Stem Cell Mesenchymal Injection Increases Platelet-Derived Growth Factors Level and Percentage of Collagen in Third-Degree Burn injured Mice |
| title_short | Stem Cell Mesenchymal Injection Increases Platelet-Derived Growth Factors Level and Percentage of Collagen in Third-Degree Burn injured Mice |
| title_sort | stem cell mesenchymal injection increases platelet derived growth factors level and percentage of collagen in third degree burn injured mice |
| topic | Mesenchymal stem cells PDGF Collagen. |
| url | http://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/1173 |
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