Clinical Characteristics of Mitochondrial DNA Copy Number in Osteonecrosis of the Femoral Head
<i>Background and objectives</i>: Alterations in mitochondrial DNA (mtDNA) have been observed and studied in various diseases. However, the clinical value of the mtDNA copy number (mtDNA-CN) alterations in osteonecrosis of the femoral head (ONFH) is poorly understood. In the present stud...
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MDPI AG
2020-05-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1010-660X/56/5/239 |
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| author | Si-Wook Lee Kyung-Jae Lee Beom-Soo Kim Hyuk-Jun Kwon Jae-Ho Lee |
| author_facet | Si-Wook Lee Kyung-Jae Lee Beom-Soo Kim Hyuk-Jun Kwon Jae-Ho Lee |
| author_sort | Si-Wook Lee |
| collection | DOAJ |
| description | <i>Background and objectives</i>: Alterations in mitochondrial DNA (mtDNA) have been observed and studied in various diseases. However, the clinical value of the mtDNA copy number (mtDNA-CN) alterations in osteonecrosis of the femoral head (ONFH) is poorly understood. In the present study, we investigated whether alterations in mtDNA-CNs are associated with clinicopathological parameters in ONFH. <i>Materials and methods</i>: MtDNA-CNs in the synovial tissue of 34 patients with ONFH and 123 control tissues (femoral neck fracture) were measured using quantitative real-time PCR. The present study then analyzed the correlation between the mtDNA-CN and the clinicopathological characteristics of ONFH and fracture patients. <i>Results</i>: The average mtDNA-CN (mean ± standard deviation) was 23.82 ± 22.37 and 25.04 ± 24.27 in ONFH and control tissues, respectively, and was not significantly different between the groups (<i>p</i> = 0.792). The mtDNA-CN was positively associated with age (27.7% vs. 45.9%, <i>p</i> = 0.018) and negatively associated with the erythrocyte sedimentation rate (ESR) (11.8% vs. 39.7%, <i>p</i> = 0.024) in all of the samples. The study also found further associations with age (22.2% vs. 68.8%, <i>p</i> = 0.014), gender (30.0% vs. 64.3%, <i>p</i> = 0.048), and ESR (0% vs. 57.7%, <i>p</i> = 0.043) in ONFH. <i>Conclusions</i>: in this study, we demonstrated that mtDNA-CN might be a significant marker for predicting clinical characteristics in ONFH. |
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| series | Medicina |
| spelling | doaj.art-9db7db2123794acca44eaf73ca7108822023-09-02T14:27:15ZengMDPI AGMedicina1010-660X2020-05-015623923910.3390/medicina56050239Clinical Characteristics of Mitochondrial DNA Copy Number in Osteonecrosis of the Femoral HeadSi-Wook Lee0Kyung-Jae Lee1Beom-Soo Kim2Hyuk-Jun Kwon3Jae-Ho Lee4Department of Orthopaedic Surgery, Keimyung University School of Medicine, Dongsan Hospital, Daegu 42601, KoreaDepartment of Orthopaedic Surgery, Keimyung University School of Medicine, Dongsan Hospital, Daegu 42601, KoreaDepartment of Orthopaedic Surgery, Keimyung University School of Medicine, Dongsan Hospital, Daegu 42601, KoreaDepartment of Orthopaedic Surgery, Keimyung University School of Medicine, Dongsan Hospital, Daegu 42601, KoreaDepartment of Anatomy, School of Medicine, Keimyung University, Daegu 42601, Korea<i>Background and objectives</i>: Alterations in mitochondrial DNA (mtDNA) have been observed and studied in various diseases. However, the clinical value of the mtDNA copy number (mtDNA-CN) alterations in osteonecrosis of the femoral head (ONFH) is poorly understood. In the present study, we investigated whether alterations in mtDNA-CNs are associated with clinicopathological parameters in ONFH. <i>Materials and methods</i>: MtDNA-CNs in the synovial tissue of 34 patients with ONFH and 123 control tissues (femoral neck fracture) were measured using quantitative real-time PCR. The present study then analyzed the correlation between the mtDNA-CN and the clinicopathological characteristics of ONFH and fracture patients. <i>Results</i>: The average mtDNA-CN (mean ± standard deviation) was 23.82 ± 22.37 and 25.04 ± 24.27 in ONFH and control tissues, respectively, and was not significantly different between the groups (<i>p</i> = 0.792). The mtDNA-CN was positively associated with age (27.7% vs. 45.9%, <i>p</i> = 0.018) and negatively associated with the erythrocyte sedimentation rate (ESR) (11.8% vs. 39.7%, <i>p</i> = 0.024) in all of the samples. The study also found further associations with age (22.2% vs. 68.8%, <i>p</i> = 0.014), gender (30.0% vs. 64.3%, <i>p</i> = 0.048), and ESR (0% vs. 57.7%, <i>p</i> = 0.043) in ONFH. <i>Conclusions</i>: in this study, we demonstrated that mtDNA-CN might be a significant marker for predicting clinical characteristics in ONFH.https://www.mdpi.com/1010-660X/56/5/239mtDNAmtDNA-CNONFHosteonecrosismitochondrial DNAESR |
| spellingShingle | Si-Wook Lee Kyung-Jae Lee Beom-Soo Kim Hyuk-Jun Kwon Jae-Ho Lee Clinical Characteristics of Mitochondrial DNA Copy Number in Osteonecrosis of the Femoral Head Medicina mtDNA mtDNA-CN ONFH osteonecrosis mitochondrial DNA ESR |
| title | Clinical Characteristics of Mitochondrial DNA Copy Number in Osteonecrosis of the Femoral Head |
| title_full | Clinical Characteristics of Mitochondrial DNA Copy Number in Osteonecrosis of the Femoral Head |
| title_fullStr | Clinical Characteristics of Mitochondrial DNA Copy Number in Osteonecrosis of the Femoral Head |
| title_full_unstemmed | Clinical Characteristics of Mitochondrial DNA Copy Number in Osteonecrosis of the Femoral Head |
| title_short | Clinical Characteristics of Mitochondrial DNA Copy Number in Osteonecrosis of the Femoral Head |
| title_sort | clinical characteristics of mitochondrial dna copy number in osteonecrosis of the femoral head |
| topic | mtDNA mtDNA-CN ONFH osteonecrosis mitochondrial DNA ESR |
| url | https://www.mdpi.com/1010-660X/56/5/239 |
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