Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats
PURPOSE:To evaluate the effects of acupuncture (Ac) and electroacupuncture (EAc) on oxidative stress and inflammation in testis torsion/detorsion (T/D) model in rats.METHODS: Thirty male Wistar rats were randomized into five groups. G1 Group (Sham) served as control. The remaining groups were submit...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2014-07-01
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| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000700450&tlng=en |
| _version_ | 1818491018705633280 |
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| author | Porfirio Cezar Passos Acioli Andrea de Oliveira Albuquerque Isaac Bevilaqua de Albuquerque Guimarães Roberto Wagner Bezerra de Araujo Paulo Roberto Leitão Vasconcelos Sérgio Botelho Guimarães |
| author_facet | Porfirio Cezar Passos Acioli Andrea de Oliveira Albuquerque Isaac Bevilaqua de Albuquerque Guimarães Roberto Wagner Bezerra de Araujo Paulo Roberto Leitão Vasconcelos Sérgio Botelho Guimarães |
| author_sort | Porfirio Cezar Passos Acioli |
| collection | DOAJ |
| description | PURPOSE:To evaluate the effects of acupuncture (Ac) and electroacupuncture (EAc) on oxidative stress and inflammation in testis torsion/detorsion (T/D) model in rats.METHODS: Thirty male Wistar rats were randomized into five groups. G1 Group (Sham) served as control. The remaining groups were submitted to spermatic cord torsion (720°) for 3 hours, followed by detorsion and reperfusion for 4 hours. Before detorsion G3, G4 and G5 rats were treated with Ac, EAc 2Hz and EAc 10 Hz, respectively, applied to acupoint Gulai (S-29) bilaterally under anesthesia for 5 minutes. Next, the testes were detorsioned and reperfused for 4 hours. Afterwards, blood samples and the right testis were collected for biochemical assays: reduced Glutathione (GSH), Malonaldehyde (MDA), Myeloperoxidase (MPO).RESULTS: EAc stimulation (2 and 10 Hz) promoted significant increase in concentrations of GSH in plasma and testis of G4-G5 rats, compared with G1. There was significant increase of tissue MDA in groups G4-G5 and plasma MDA in all groups, compared with G1. There was a significant reduction in MPO activity in groups G4-G5 compared with G1.CONCLUSION:Electroacupuncture stimulation (2 and 10 Hz) attenuates oxidative stress and inflammatory response in rats subjected to testicular torsion/detorsion. |
| first_indexed | 2024-12-10T17:24:42Z |
| format | Article |
| id | doaj.art-9db89b78e0534ec6a9a33116cf65017a |
| institution | Directory Open Access Journal |
| issn | 0102-8650 |
| language | English |
| last_indexed | 2024-12-10T17:24:42Z |
| publishDate | 2014-07-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-9db89b78e0534ec6a9a33116cf65017a2022-12-22T01:39:53ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira0102-86502014-07-0129745045610.1590/S0102-86502014000700007Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in ratsPorfirio Cezar Passos AcioliAndrea de Oliveira AlbuquerqueIsaac Bevilaqua de Albuquerque GuimarãesRoberto Wagner Bezerra de AraujoPaulo Roberto Leitão VasconcelosSérgio Botelho GuimarãesPURPOSE:To evaluate the effects of acupuncture (Ac) and electroacupuncture (EAc) on oxidative stress and inflammation in testis torsion/detorsion (T/D) model in rats.METHODS: Thirty male Wistar rats were randomized into five groups. G1 Group (Sham) served as control. The remaining groups were submitted to spermatic cord torsion (720°) for 3 hours, followed by detorsion and reperfusion for 4 hours. Before detorsion G3, G4 and G5 rats were treated with Ac, EAc 2Hz and EAc 10 Hz, respectively, applied to acupoint Gulai (S-29) bilaterally under anesthesia for 5 minutes. Next, the testes were detorsioned and reperfused for 4 hours. Afterwards, blood samples and the right testis were collected for biochemical assays: reduced Glutathione (GSH), Malonaldehyde (MDA), Myeloperoxidase (MPO).RESULTS: EAc stimulation (2 and 10 Hz) promoted significant increase in concentrations of GSH in plasma and testis of G4-G5 rats, compared with G1. There was significant increase of tissue MDA in groups G4-G5 and plasma MDA in all groups, compared with G1. There was a significant reduction in MPO activity in groups G4-G5 compared with G1.CONCLUSION:Electroacupuncture stimulation (2 and 10 Hz) attenuates oxidative stress and inflammatory response in rats subjected to testicular torsion/detorsion.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000700450&tlng=enSpermatic Cord TorsionIschemiaReperfusion InjuryElectroacupunctureRats |
| spellingShingle | Porfirio Cezar Passos Acioli Andrea de Oliveira Albuquerque Isaac Bevilaqua de Albuquerque Guimarães Roberto Wagner Bezerra de Araujo Paulo Roberto Leitão Vasconcelos Sérgio Botelho Guimarães Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats Acta Cirúrgica Brasileira Spermatic Cord Torsion Ischemia Reperfusion Injury Electroacupuncture Rats |
| title | Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats |
| title_full | Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats |
| title_fullStr | Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats |
| title_full_unstemmed | Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats |
| title_short | Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats |
| title_sort | protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion detorsion in rats |
| topic | Spermatic Cord Torsion Ischemia Reperfusion Injury Electroacupuncture Rats |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000700450&tlng=en |
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