Association between <i>HSPA8</i> Gene Variants and Ischemic Stroke: A Pilot Study Providing Additional Evidence for the Role of Heat Shock Proteins in Disease Pathogenesis
HSPA8 is involved in many stroke-associated cellular processes, playing a pivotal role in the protein quality control system. Here we report the results of the pilot study aimed at determining whether <i>HSPA8</i> SNPs are linked to the risk of ischemic stroke (IS). DNA samples from 2139...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/6/1171 |
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| author | Ksenia A. Kobzeva Maria O. Soldatova Tatiana A. Stetskaya Vladislav O. Soldatov Alexey V. Deykin Maxim B. Freidin Marina A. Bykanova Mikhail I. Churnosov Alexey V. Polonikov Olga Y. Bushueva |
| author_facet | Ksenia A. Kobzeva Maria O. Soldatova Tatiana A. Stetskaya Vladislav O. Soldatov Alexey V. Deykin Maxim B. Freidin Marina A. Bykanova Mikhail I. Churnosov Alexey V. Polonikov Olga Y. Bushueva |
| author_sort | Ksenia A. Kobzeva |
| collection | DOAJ |
| description | HSPA8 is involved in many stroke-associated cellular processes, playing a pivotal role in the protein quality control system. Here we report the results of the pilot study aimed at determining whether <i>HSPA8</i> SNPs are linked to the risk of ischemic stroke (IS). DNA samples from 2139 Russians (888 IS patients and 1251 healthy controls) were genotyped for tagSNPs (rs1461496, rs10892958, and rs1136141) in the <i>HSPA8</i> gene using probe-based PCR. SNP rs10892958 of <i>HSPA8</i> was associated with an increased risk (risk allele G) of IS in smokers (OR = 1.37; 95% CI = 1.07–1.77; <i>p</i> = 0.01) and patients with low fruit and vegetable consumption (OR = 1.36; 95% CI = 1.14–1.63; <i>p</i> = 0.002). SNP rs1136141 of <i>HSPA8</i> was also associated with an increased risk of IS (risk allele A) exclusively in smokers (OR = 1.68; 95% CI = 1.23–2.28; <i>p</i> = 0.0007) and in patients with a low fruit and vegetable intake (OR = 1.29; 95% CI = 1.05–1.60; <i>p</i> = 0.04). Sex-stratified analysis revealed an association of rs10892958 <i>HSPA8</i> with an increased risk of IS in males (risk allele G; OR = 1.30; 95% CI = 1.05–1.61; <i>p</i> = 0.01). Thus, SNPs rs10892958 and rs1136141 in the <i>HSPA8</i> gene represent novel genetic markers of IS. |
| first_indexed | 2024-03-11T02:25:35Z |
| format | Article |
| id | doaj.art-9dbbe407cbfd45ce9a5ba1bef1e00752 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T02:25:35Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-9dbbe407cbfd45ce9a5ba1bef1e007522023-11-18T10:33:47ZengMDPI AGGenes2073-44252023-05-01146117110.3390/genes14061171Association between <i>HSPA8</i> Gene Variants and Ischemic Stroke: A Pilot Study Providing Additional Evidence for the Role of Heat Shock Proteins in Disease PathogenesisKsenia A. Kobzeva0Maria O. Soldatova1Tatiana A. Stetskaya2Vladislav O. Soldatov3Alexey V. Deykin4Maxim B. Freidin5Marina A. Bykanova6Mikhail I. Churnosov7Alexey V. Polonikov8Olga Y. Bushueva9Laboratory of Genomic Research, Research Institute for Genetic and Molecular Epidemiology, Kursk State Medical University, 305041 Kursk, RussiaLaboratory of Genomic Research, Research Institute for Genetic and Molecular Epidemiology, Kursk State Medical University, 305041 Kursk, RussiaLaboratory of Statistical Genetics and Bioinformatics, Research Institute for Genetic and Molecular Epidemiology, Kursk State Medical University, 305041 Kursk, RussiaLaboratory of Genome Editing for Biomedicine and Animal Health, Belgorod State National Research University, 308015 Belgorod, RussiaLaboratory of Genome Editing for Biomedicine and Animal Health, Belgorod State National Research University, 308015 Belgorod, RussiaDepartment of Biology, School of Biological and Behavioural Sciences, Queen Mary University of London, London E1 4NS, UKLaboratory of Genomic Research, Research Institute for Genetic and Molecular Epidemiology, Kursk State Medical University, 305041 Kursk, RussiaDepartment of Medical Biological Disciplines, Belgorod State University, 308015 Belgorod, RussiaLaboratory of Statistical Genetics and Bioinformatics, Research Institute for Genetic and Molecular Epidemiology, Kursk State Medical University, 305041 Kursk, RussiaLaboratory of Genomic Research, Research Institute for Genetic and Molecular Epidemiology, Kursk State Medical University, 305041 Kursk, RussiaHSPA8 is involved in many stroke-associated cellular processes, playing a pivotal role in the protein quality control system. Here we report the results of the pilot study aimed at determining whether <i>HSPA8</i> SNPs are linked to the risk of ischemic stroke (IS). DNA samples from 2139 Russians (888 IS patients and 1251 healthy controls) were genotyped for tagSNPs (rs1461496, rs10892958, and rs1136141) in the <i>HSPA8</i> gene using probe-based PCR. SNP rs10892958 of <i>HSPA8</i> was associated with an increased risk (risk allele G) of IS in smokers (OR = 1.37; 95% CI = 1.07–1.77; <i>p</i> = 0.01) and patients with low fruit and vegetable consumption (OR = 1.36; 95% CI = 1.14–1.63; <i>p</i> = 0.002). SNP rs1136141 of <i>HSPA8</i> was also associated with an increased risk of IS (risk allele A) exclusively in smokers (OR = 1.68; 95% CI = 1.23–2.28; <i>p</i> = 0.0007) and in patients with a low fruit and vegetable intake (OR = 1.29; 95% CI = 1.05–1.60; <i>p</i> = 0.04). Sex-stratified analysis revealed an association of rs10892958 <i>HSPA8</i> with an increased risk of IS in males (risk allele G; OR = 1.30; 95% CI = 1.05–1.61; <i>p</i> = 0.01). Thus, SNPs rs10892958 and rs1136141 in the <i>HSPA8</i> gene represent novel genetic markers of IS.https://www.mdpi.com/2073-4425/14/6/1171ischemic strokechaperonesheat shock proteins<i>HSPA8</i><i>HSC70</i>rs10892958 |
| spellingShingle | Ksenia A. Kobzeva Maria O. Soldatova Tatiana A. Stetskaya Vladislav O. Soldatov Alexey V. Deykin Maxim B. Freidin Marina A. Bykanova Mikhail I. Churnosov Alexey V. Polonikov Olga Y. Bushueva Association between <i>HSPA8</i> Gene Variants and Ischemic Stroke: A Pilot Study Providing Additional Evidence for the Role of Heat Shock Proteins in Disease Pathogenesis Genes ischemic stroke chaperones heat shock proteins <i>HSPA8</i> <i>HSC70</i> rs10892958 |
| title | Association between <i>HSPA8</i> Gene Variants and Ischemic Stroke: A Pilot Study Providing Additional Evidence for the Role of Heat Shock Proteins in Disease Pathogenesis |
| title_full | Association between <i>HSPA8</i> Gene Variants and Ischemic Stroke: A Pilot Study Providing Additional Evidence for the Role of Heat Shock Proteins in Disease Pathogenesis |
| title_fullStr | Association between <i>HSPA8</i> Gene Variants and Ischemic Stroke: A Pilot Study Providing Additional Evidence for the Role of Heat Shock Proteins in Disease Pathogenesis |
| title_full_unstemmed | Association between <i>HSPA8</i> Gene Variants and Ischemic Stroke: A Pilot Study Providing Additional Evidence for the Role of Heat Shock Proteins in Disease Pathogenesis |
| title_short | Association between <i>HSPA8</i> Gene Variants and Ischemic Stroke: A Pilot Study Providing Additional Evidence for the Role of Heat Shock Proteins in Disease Pathogenesis |
| title_sort | association between i hspa8 i gene variants and ischemic stroke a pilot study providing additional evidence for the role of heat shock proteins in disease pathogenesis |
| topic | ischemic stroke chaperones heat shock proteins <i>HSPA8</i> <i>HSC70</i> rs10892958 |
| url | https://www.mdpi.com/2073-4425/14/6/1171 |
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