Assay of Antioxidant Potential of Two Filamentous Fungi Isolated from the Indonesian Fermented Dried Cassava
The antioxidant capacity and antioxidant constituents of two filamentous fungi (Acremonium charticola and Rhizopus oryzae) isolated from the Indonesian fermented dried cassava (gathot) were evaluated in the present study. The antioxidant capacity of the fungal crude extracts was assessed based on th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-02-01
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| Series: | Antioxidants |
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| Online Access: | http://www.mdpi.com/2076-3921/5/1/6 |
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| author | Sugiharto Sugiharto Turrini Yudiarti Isroli Isroli |
| author_facet | Sugiharto Sugiharto Turrini Yudiarti Isroli Isroli |
| author_sort | Sugiharto Sugiharto |
| collection | DOAJ |
| description | The antioxidant capacity and antioxidant constituents of two filamentous fungi (Acremonium charticola and Rhizopus oryzae) isolated from the Indonesian fermented dried cassava (gathot) were evaluated in the present study. The antioxidant capacity of the fungal crude extracts was assessed based on the 2,2′-azino-bis(3-ethyl-benzthiazolin-6-sulfonicacid) (ABTS) method. Total phenolics were determined based on the Folin-Ciocalteu method, while the flavonoids content in the fungal extracts was determined by the spectrophotometric method with aluminum chloride. Total tannins were estimated by the Folin-Denis method. The ABTS+ radical scavenging activity was higher (p < 0.01) in A. charticola compared to that in R. oryzae and ascorbic acid (as a control). A higher (p < 0.01) content of total phenolics was detected in A. charticola than that in R. oryzae. Total flavonoids were higher (p < 0.01) in R. oryzae as compared with that in A. charticola. The fungus A. charticola had a higher (p < 0.01) level of total tannins than R. oryzae. In conclusion, both filamentous fungi isolated from the Indonesian fermented dried cassava exhibited antioxidant potentials as indicated by their capabilities to scavenge ABTS+. A. charticola had a higher antioxidant capacity than R. oryzae. The antioxidant capacity of A. charticola was attributed mainly to its phenolics and tannins contents. |
| first_indexed | 2024-03-12T09:58:37Z |
| format | Article |
| id | doaj.art-9dbc6f9e1d324deab6387b975ed7d11b |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T09:58:37Z |
| publishDate | 2016-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-9dbc6f9e1d324deab6387b975ed7d11b2023-09-02T11:59:53ZengMDPI AGAntioxidants2076-39212016-02-0151610.3390/antiox5010006antiox5010006Assay of Antioxidant Potential of Two Filamentous Fungi Isolated from the Indonesian Fermented Dried CassavaSugiharto Sugiharto0Turrini Yudiarti1Isroli Isroli2Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang 50275, IndonesiaFaculty of Animal and Agricultural Sciences, Diponegoro University, Semarang 50275, IndonesiaFaculty of Animal and Agricultural Sciences, Diponegoro University, Semarang 50275, IndonesiaThe antioxidant capacity and antioxidant constituents of two filamentous fungi (Acremonium charticola and Rhizopus oryzae) isolated from the Indonesian fermented dried cassava (gathot) were evaluated in the present study. The antioxidant capacity of the fungal crude extracts was assessed based on the 2,2′-azino-bis(3-ethyl-benzthiazolin-6-sulfonicacid) (ABTS) method. Total phenolics were determined based on the Folin-Ciocalteu method, while the flavonoids content in the fungal extracts was determined by the spectrophotometric method with aluminum chloride. Total tannins were estimated by the Folin-Denis method. The ABTS+ radical scavenging activity was higher (p < 0.01) in A. charticola compared to that in R. oryzae and ascorbic acid (as a control). A higher (p < 0.01) content of total phenolics was detected in A. charticola than that in R. oryzae. Total flavonoids were higher (p < 0.01) in R. oryzae as compared with that in A. charticola. The fungus A. charticola had a higher (p < 0.01) level of total tannins than R. oryzae. In conclusion, both filamentous fungi isolated from the Indonesian fermented dried cassava exhibited antioxidant potentials as indicated by their capabilities to scavenge ABTS+. A. charticola had a higher antioxidant capacity than R. oryzae. The antioxidant capacity of A. charticola was attributed mainly to its phenolics and tannins contents.http://www.mdpi.com/2076-3921/5/1/6antioxidant potentialantioxidant constituentsAcremonium charticolaRhizopus oryzaegathot |
| spellingShingle | Sugiharto Sugiharto Turrini Yudiarti Isroli Isroli Assay of Antioxidant Potential of Two Filamentous Fungi Isolated from the Indonesian Fermented Dried Cassava Antioxidants antioxidant potential antioxidant constituents Acremonium charticola Rhizopus oryzae gathot |
| title | Assay of Antioxidant Potential of Two Filamentous Fungi Isolated from the Indonesian Fermented Dried Cassava |
| title_full | Assay of Antioxidant Potential of Two Filamentous Fungi Isolated from the Indonesian Fermented Dried Cassava |
| title_fullStr | Assay of Antioxidant Potential of Two Filamentous Fungi Isolated from the Indonesian Fermented Dried Cassava |
| title_full_unstemmed | Assay of Antioxidant Potential of Two Filamentous Fungi Isolated from the Indonesian Fermented Dried Cassava |
| title_short | Assay of Antioxidant Potential of Two Filamentous Fungi Isolated from the Indonesian Fermented Dried Cassava |
| title_sort | assay of antioxidant potential of two filamentous fungi isolated from the indonesian fermented dried cassava |
| topic | antioxidant potential antioxidant constituents Acremonium charticola Rhizopus oryzae gathot |
| url | http://www.mdpi.com/2076-3921/5/1/6 |
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