Bioactivity Assessment of Indian Origin—Mangrove Actinobacteria against Candida albicans
Actinobacteria is found to have a potent metabolic activity against pathogens. The present study reveals the assessment of potent antifungal secondary metabolites from actinobacteria isolated from Indian marine mangrove sediments. The samples were collected from the coastal regions of Muthupet, Anda...
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MDPI AG
2018-02-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/16/2/60 |
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| author | J. G. S. Pavan Kumar Ajitha Gomathi Vitor Vasconcelos K. M. Gothandam |
| author_facet | J. G. S. Pavan Kumar Ajitha Gomathi Vitor Vasconcelos K. M. Gothandam |
| author_sort | J. G. S. Pavan Kumar |
| collection | DOAJ |
| description | Actinobacteria is found to have a potent metabolic activity against pathogens. The present study reveals the assessment of potent antifungal secondary metabolites from actinobacteria isolated from Indian marine mangrove sediments. The samples were collected from the coastal regions of Muthupet, Andaman and the Nicobar Islands. Identification was carried out using 16S rRNA analysis and biosynthetic genes (Polyketide synthase type I/II and Non-ribosomal peptide synthase) were screened. Actinobacteria were assayed for their antifungal activity against 16 clinical Candida albicans and the compound analysis was performed using gas chromatography-mass spectrometry GC-MS. The 31 actinobacterial strains were isolated and 16S rRNA gene sequencing revealed that this ecosystem is rich on actinobacteria, with Streptomyces as the predominant genus. The PCR based screening of biosynthetic genes revealed the presence of PKS-I in six strains, PKS-II in four strains and NRPS in 11 strains. The isolated actinobacteria VITGAP240 and VITGAP241 (two isolates) were found to have a potential antifungal activity against all the tested C. albicans. GC-MS results revealed that the actinobacterial compounds were belonging to heterocyclic, polyketides and peptides. Overall, the strains possess a wide spectrum of antifungal properties which affords the production of significant bioactive metabolites as potential antibiotics. |
| first_indexed | 2024-04-11T13:26:57Z |
| format | Article |
| id | doaj.art-9dbea97930814e1a8acbdb509ae6848b |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T13:26:57Z |
| publishDate | 2018-02-01 |
| publisher | MDPI AG |
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| series | Marine Drugs |
| spelling | doaj.art-9dbea97930814e1a8acbdb509ae6848b2022-12-22T04:22:00ZengMDPI AGMarine Drugs1660-33972018-02-011626010.3390/md16020060md16020060Bioactivity Assessment of Indian Origin—Mangrove Actinobacteria against Candida albicansJ. G. S. Pavan Kumar0Ajitha Gomathi1Vitor Vasconcelos2K. M. Gothandam3Department of Biotechnology, School of Biosciences and Technology, VIT University, Vellore 632014, IndiaDepartment of Biotechnology, School of Biosciences and Technology, VIT University, Vellore 632014, IndiaCIIMAR/CIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, PortugalDepartment of Biotechnology, School of Biosciences and Technology, VIT University, Vellore 632014, IndiaActinobacteria is found to have a potent metabolic activity against pathogens. The present study reveals the assessment of potent antifungal secondary metabolites from actinobacteria isolated from Indian marine mangrove sediments. The samples were collected from the coastal regions of Muthupet, Andaman and the Nicobar Islands. Identification was carried out using 16S rRNA analysis and biosynthetic genes (Polyketide synthase type I/II and Non-ribosomal peptide synthase) were screened. Actinobacteria were assayed for their antifungal activity against 16 clinical Candida albicans and the compound analysis was performed using gas chromatography-mass spectrometry GC-MS. The 31 actinobacterial strains were isolated and 16S rRNA gene sequencing revealed that this ecosystem is rich on actinobacteria, with Streptomyces as the predominant genus. The PCR based screening of biosynthetic genes revealed the presence of PKS-I in six strains, PKS-II in four strains and NRPS in 11 strains. The isolated actinobacteria VITGAP240 and VITGAP241 (two isolates) were found to have a potential antifungal activity against all the tested C. albicans. GC-MS results revealed that the actinobacterial compounds were belonging to heterocyclic, polyketides and peptides. Overall, the strains possess a wide spectrum of antifungal properties which affords the production of significant bioactive metabolites as potential antibiotics.http://www.mdpi.com/1660-3397/16/2/60actinobacteriaantifungalpolyketide synthasenon ribosomal peptide synthaseCandida albicans |
| spellingShingle | J. G. S. Pavan Kumar Ajitha Gomathi Vitor Vasconcelos K. M. Gothandam Bioactivity Assessment of Indian Origin—Mangrove Actinobacteria against Candida albicans Marine Drugs actinobacteria antifungal polyketide synthase non ribosomal peptide synthase Candida albicans |
| title | Bioactivity Assessment of Indian Origin—Mangrove Actinobacteria against Candida albicans |
| title_full | Bioactivity Assessment of Indian Origin—Mangrove Actinobacteria against Candida albicans |
| title_fullStr | Bioactivity Assessment of Indian Origin—Mangrove Actinobacteria against Candida albicans |
| title_full_unstemmed | Bioactivity Assessment of Indian Origin—Mangrove Actinobacteria against Candida albicans |
| title_short | Bioactivity Assessment of Indian Origin—Mangrove Actinobacteria against Candida albicans |
| title_sort | bioactivity assessment of indian origin mangrove actinobacteria against candida albicans |
| topic | actinobacteria antifungal polyketide synthase non ribosomal peptide synthase Candida albicans |
| url | http://www.mdpi.com/1660-3397/16/2/60 |
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