Identification of deer and goat species from skin samples-a DNA barcoding approach
DNA barcoding is a technique for characterizing species of organisms using a short DNA sequence from a standard and agreed-upon position in the genome. In the present study, DNA barcoding was used as a technology to differentiate deer and goat species from the skin samples. The samples were collecte...
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| Format: | Article |
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Director of Academics and Research, Kerala Veterinary and Animal Sciences University
2021-01-01
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| Series: | Journal of Veterinary and Animal Sciences |
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| Online Access: | https://www.jvas.in/public_html/upload/article_file/article_file_qutk2x.pdf?t=qutk2x |
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| author | S. Maya Sanil George A.R. Sreeranjini C. Leena N. S. Sunilkumar K.B. Sumena |
| author_facet | S. Maya Sanil George A.R. Sreeranjini C. Leena N. S. Sunilkumar K.B. Sumena |
| author_sort | S. Maya |
| collection | DOAJ |
| description | DNA barcoding is a technique for characterizing species of organisms using a short DNA sequence from a standard and agreed-upon position in the genome. In the present study, DNA barcoding was used as a technology to differentiate deer and goat species from the skin samples. The samples were collected from different regions of the skin, viz. ear pinna, dorsal abdomen, muzzle and interdigital space of foerelimb of deer brought for post mortem at College of Veterinary and Animal Sciences, from Thrissur zoo and forest department and of goat freshly slaughtered in Meat Technology Unit, Mannuthy. Samples were chopped and preserved in ethanol and processed. The mitochondrial sequence of Cytochrome C Oxidase subunit (COI) was deliberated as a barcode marker suited best for species identification of the animals and based on this the samples were identified as Cervus unicolor, Rusa timorensis and Capra hircus. The results recommended that unidentified specimens from wild animals can be recognized competently using DNA sequencebased analysis. |
| first_indexed | 2024-12-21T17:28:15Z |
| format | Article |
| id | doaj.art-9dbee8aec5dc45e8bd8b0b48df745756 |
| institution | Directory Open Access Journal |
| issn | 0971-0701 2582-0605 |
| language | English |
| last_indexed | 2024-12-21T17:28:15Z |
| publishDate | 2021-01-01 |
| publisher | Director of Academics and Research, Kerala Veterinary and Animal Sciences University |
| record_format | Article |
| series | Journal of Veterinary and Animal Sciences |
| spelling | doaj.art-9dbee8aec5dc45e8bd8b0b48df7457562022-12-21T18:55:59ZengDirector of Academics and Research, Kerala Veterinary and Animal Sciences UniversityJournal of Veterinary and Animal Sciences0971-07012582-06052021-01-01521858710.51966/jvas.2021.52.1.85-87Identification of deer and goat species from skin samples-a DNA barcoding approachS. Maya0https://orcid.org/0000-0001-9111-1897Sanil George1A.R. Sreeranjini2https://orcid.org/0000-0001-7384-1570C. Leena3N. S. Sunilkumar4https://orcid.org/0000-0002-3785-5825K.B. Sumena5Department of Veterinary Anatomy and Histology, College of Veterinary and Animal Sciences, Mannuthy. Kerala Veterinary and Animal Sciences University, Pookode.Rajiv Gandhi Centre of Biotechnology, Thiruvananthapuram, Kerala-695014Department of Veterinary Anatomy and Histology, College of Veterinary and Animal Sciences, Mannuthy. Kerala Veterinary and Animal Sciences University, Pookode.Department of Veterinary Anatomy and Histology, College of Veterinary and Animal Sciences, Mannuthy. Kerala Veterinary and Animal Sciences University, Pookode.Department of Veterinary Anatomy and Histology, College of Veterinary and Animal Sciences, Mannuthy. Kerala Veterinary and Animal Sciences University, Pookode.Department of Veterinary Anatomy and Histology, College of Veterinary and Animal Sciences, Mannuthy. Kerala Veterinary and Animal Sciences University, Pookode.DNA barcoding is a technique for characterizing species of organisms using a short DNA sequence from a standard and agreed-upon position in the genome. In the present study, DNA barcoding was used as a technology to differentiate deer and goat species from the skin samples. The samples were collected from different regions of the skin, viz. ear pinna, dorsal abdomen, muzzle and interdigital space of foerelimb of deer brought for post mortem at College of Veterinary and Animal Sciences, from Thrissur zoo and forest department and of goat freshly slaughtered in Meat Technology Unit, Mannuthy. Samples were chopped and preserved in ethanol and processed. The mitochondrial sequence of Cytochrome C Oxidase subunit (COI) was deliberated as a barcode marker suited best for species identification of the animals and based on this the samples were identified as Cervus unicolor, Rusa timorensis and Capra hircus. The results recommended that unidentified specimens from wild animals can be recognized competently using DNA sequencebased analysis.https://www.jvas.in/public_html/upload/article_file/article_file_qutk2x.pdf?t=qutk2xdeerdna barcodinggoatskin |
| spellingShingle | S. Maya Sanil George A.R. Sreeranjini C. Leena N. S. Sunilkumar K.B. Sumena Identification of deer and goat species from skin samples-a DNA barcoding approach Journal of Veterinary and Animal Sciences deer dna barcoding goat skin |
| title | Identification of deer and goat species from skin samples-a DNA barcoding approach |
| title_full | Identification of deer and goat species from skin samples-a DNA barcoding approach |
| title_fullStr | Identification of deer and goat species from skin samples-a DNA barcoding approach |
| title_full_unstemmed | Identification of deer and goat species from skin samples-a DNA barcoding approach |
| title_short | Identification of deer and goat species from skin samples-a DNA barcoding approach |
| title_sort | identification of deer and goat species from skin samples a dna barcoding approach |
| topic | deer dna barcoding goat skin |
| url | https://www.jvas.in/public_html/upload/article_file/article_file_qutk2x.pdf?t=qutk2x |
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