Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass
Single-cell RNA sequencing (scRNA-seq) is widely adopted for identifying the signature molecular markers or regulators in cells, as this would benefit defining or isolating various types of cells. Likewise, the signature transcriptome profile analysis at the single cell level would well illustrate t...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-03-01
|
| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.647892/full |
| _version_ | 1818667880490729472 |
|---|---|
| author | Xiaoli Liu Xiaoli Liu Wei Li Yanping Yang Kaili Chen Yulin Li Xinping Zhu Hua Ye Hongyan Xu Hongyan Xu |
| author_facet | Xiaoli Liu Xiaoli Liu Wei Li Yanping Yang Kaili Chen Yulin Li Xinping Zhu Hua Ye Hongyan Xu Hongyan Xu |
| author_sort | Xiaoli Liu |
| collection | DOAJ |
| description | Single-cell RNA sequencing (scRNA-seq) is widely adopted for identifying the signature molecular markers or regulators in cells, as this would benefit defining or isolating various types of cells. Likewise, the signature transcriptome profile analysis at the single cell level would well illustrate the key regulators or networks involved in gametogenesis and gonad development in animals; however, there is limited scRNA-seq analysis on gonadal cells in lower vertebrates, especially in the sexual reversal fish species. In this study, we analyzed the molecular signature of several distinct cell populations of Asian seabass adult ovaries through scRNA-seq. We identified five cell types and also successfully validated some specific genes of germ cells and granulosa cells. Likewise, we found some key pathways involved in ovarian development that may concert germline-somatic interactions. Moreover, we compared the transcriptomic profiles across fruit fly, mammals, and fish, and thus uncovered the conservation and divergence in molecular mechanisms that might drive ovarian development. Our results provide a basis for studying the crucial features of germ cells and somatic cells, which will benefit the understandings of the molecular mechanisms behind gametogenesis and gonad development in fish. |
| first_indexed | 2024-12-17T06:27:27Z |
| format | Article |
| id | doaj.art-9dc0c20ec8a548fba75efc12370e7f99 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-17T06:27:27Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-9dc0c20ec8a548fba75efc12370e7f992022-12-21T22:00:16ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-03-01910.3389/fcell.2021.647892647892Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian SeabassXiaoli Liu0Xiaoli Liu1Wei Li2Yanping Yang3Kaili Chen4Yulin Li5Xinping Zhu6Hua Ye7Hongyan Xu8Hongyan Xu9Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Key Laboratory of Aquatic Sciences of Chongqing, College of Fisheries, Southwest University, Chongqing, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Key Laboratory of Aquatic Sciences of Chongqing, College of Fisheries, Southwest University, Chongqing, ChinaKey Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Key Laboratory of Aquatic Sciences of Chongqing, College of Fisheries, Southwest University, Chongqing, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Key Laboratory of Aquatic Sciences of Chongqing, College of Fisheries, Southwest University, Chongqing, ChinaKey Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Key Laboratory of Aquatic Sciences of Chongqing, College of Fisheries, Southwest University, Chongqing, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaSingle-cell RNA sequencing (scRNA-seq) is widely adopted for identifying the signature molecular markers or regulators in cells, as this would benefit defining or isolating various types of cells. Likewise, the signature transcriptome profile analysis at the single cell level would well illustrate the key regulators or networks involved in gametogenesis and gonad development in animals; however, there is limited scRNA-seq analysis on gonadal cells in lower vertebrates, especially in the sexual reversal fish species. In this study, we analyzed the molecular signature of several distinct cell populations of Asian seabass adult ovaries through scRNA-seq. We identified five cell types and also successfully validated some specific genes of germ cells and granulosa cells. Likewise, we found some key pathways involved in ovarian development that may concert germline-somatic interactions. Moreover, we compared the transcriptomic profiles across fruit fly, mammals, and fish, and thus uncovered the conservation and divergence in molecular mechanisms that might drive ovarian development. Our results provide a basis for studying the crucial features of germ cells and somatic cells, which will benefit the understandings of the molecular mechanisms behind gametogenesis and gonad development in fish.https://www.frontiersin.org/articles/10.3389/fcell.2021.647892/fullsingle-celltranscriptomeAsian seabassovaryaquatic animal |
| spellingShingle | Xiaoli Liu Xiaoli Liu Wei Li Yanping Yang Kaili Chen Yulin Li Xinping Zhu Hua Ye Hongyan Xu Hongyan Xu Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass Frontiers in Cell and Developmental Biology single-cell transcriptome Asian seabass ovary aquatic animal |
| title | Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass |
| title_full | Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass |
| title_fullStr | Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass |
| title_full_unstemmed | Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass |
| title_short | Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass |
| title_sort | transcriptome profiling of the ovarian cells at the single cell resolution in adult asian seabass |
| topic | single-cell transcriptome Asian seabass ovary aquatic animal |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2021.647892/full |
| work_keys_str_mv | AT xiaoliliu transcriptomeprofilingoftheovariancellsatthesinglecellresolutioninadultasianseabass AT xiaoliliu transcriptomeprofilingoftheovariancellsatthesinglecellresolutioninadultasianseabass AT weili transcriptomeprofilingoftheovariancellsatthesinglecellresolutioninadultasianseabass AT yanpingyang transcriptomeprofilingoftheovariancellsatthesinglecellresolutioninadultasianseabass AT kailichen transcriptomeprofilingoftheovariancellsatthesinglecellresolutioninadultasianseabass AT yulinli transcriptomeprofilingoftheovariancellsatthesinglecellresolutioninadultasianseabass AT xinpingzhu transcriptomeprofilingoftheovariancellsatthesinglecellresolutioninadultasianseabass AT huaye transcriptomeprofilingoftheovariancellsatthesinglecellresolutioninadultasianseabass AT hongyanxu transcriptomeprofilingoftheovariancellsatthesinglecellresolutioninadultasianseabass AT hongyanxu transcriptomeprofilingoftheovariancellsatthesinglecellresolutioninadultasianseabass |