Sentinel lymph node biopsy for conjunctival malignant melanoma: surgical techniques
Alberto JA Wainstein,1,2 Ana P Drummond-Lage,1 Milhem JM Kansaon,2 Gustavo O Bretas,2 Rodrigo F Almeida,3 Ana LF Gloria,3 Ana RP Figueiredo3 1Faculty of Medical Sciences of Minas Gerais, 2Oncad Surgical Oncology, 3Ophthalmology Department, Federal University of Minas Gerais, Belo Horizonte, Brazil B...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2014-12-01
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| Series: | Clinical Ophthalmology |
| Online Access: | http://www.dovepress.com/sentinel-lymph-node-biopsy-for-conjunctival-malignant-melanoma-surgica-peer-reviewed-article-OPTH |
| _version_ | 1819000834661285888 |
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| author | Wainstein AJA Drummond-Lage AP Kansaon MJM Bretas GO Almeida RF Gloria ALF Figueiredo ARP |
| author_facet | Wainstein AJA Drummond-Lage AP Kansaon MJM Bretas GO Almeida RF Gloria ALF Figueiredo ARP |
| author_sort | Wainstein AJA |
| collection | DOAJ |
| description | Alberto JA Wainstein,1,2 Ana P Drummond-Lage,1 Milhem JM Kansaon,2 Gustavo O Bretas,2 Rodrigo F Almeida,3 Ana LF Gloria,3 Ana RP Figueiredo3 1Faculty of Medical Sciences of Minas Gerais, 2Oncad Surgical Oncology, 3Ophthalmology Department, Federal University of Minas Gerais, Belo Horizonte, Brazil Background: The purpose of this report is to examine the viability and safety of preoperative lymphoscintigraphy and radio guided sentinel lymph node (SLN) biopsy for conjunctival melanoma, and to identify the best technique to perform this procedure.Methods: Three patients diagnosed with malignant melanoma of the conjunctiva underwent lymphoscintigraphy and SLN biopsy using a dual technique comprising isosulfan blue dye and technetium Tc 99m sulfur colloid. Each patient was anesthetized and the conjunctival melanoma was excised. SLNs were localized by a gamma probe, identified according to radioactivity and sentinel blue printing, and dissected, along with drainage of the associated lymphatic basins. The SLNs were evaluated by a pathologist using hematoxylin-eosin staining following serial sectioning and immunohistochemistry using a triple melanoma cocktail (S-100, Melan-A, and HMB-45 antigens).Results: Two SLNs were stained in the jugular chain during preoperative lymphoscintigraphy in the first patient, two SLNs were identified in the preauricular and submandibular areas in the second patient, and two SLNs were identified in the submandibular and parotid areas in the third patient. All lymph nodes identified by lymphoscintigraphy were dissected and identified at surgery with 100% accuracy in all three patients. All SLNs were histologically and immunohistochemically negative. Patients had good cosmetic and functional results, and maintained their visual acuity and ocular motility.Conclusion: Patients with conjunctival melanoma can undergo preoperative lymphoscintigraphy and SLN biopsy safely using radioactive technetium and isosulfan blue dye. Keywords: ocular melanoma, sentinel lymph node biopsy, lymphoscintigraphy, conjunctival melanoma |
| first_indexed | 2024-12-20T22:39:37Z |
| format | Article |
| id | doaj.art-9dc0ecb5202446e598cc3b11e1286e21 |
| institution | Directory Open Access Journal |
| issn | 1177-5483 |
| language | English |
| last_indexed | 2024-12-20T22:39:37Z |
| publishDate | 2014-12-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Clinical Ophthalmology |
| spelling | doaj.art-9dc0ecb5202446e598cc3b11e1286e212022-12-21T19:24:30ZengDove Medical PressClinical Ophthalmology1177-54832014-12-012015default1619598Sentinel lymph node biopsy for conjunctival malignant melanoma: surgical techniquesWainstein AJADrummond-Lage APKansaon MJMBretas GOAlmeida RFGloria ALFFigueiredo ARPAlberto JA Wainstein,1,2 Ana P Drummond-Lage,1 Milhem JM Kansaon,2 Gustavo O Bretas,2 Rodrigo F Almeida,3 Ana LF Gloria,3 Ana RP Figueiredo3 1Faculty of Medical Sciences of Minas Gerais, 2Oncad Surgical Oncology, 3Ophthalmology Department, Federal University of Minas Gerais, Belo Horizonte, Brazil Background: The purpose of this report is to examine the viability and safety of preoperative lymphoscintigraphy and radio guided sentinel lymph node (SLN) biopsy for conjunctival melanoma, and to identify the best technique to perform this procedure.Methods: Three patients diagnosed with malignant melanoma of the conjunctiva underwent lymphoscintigraphy and SLN biopsy using a dual technique comprising isosulfan blue dye and technetium Tc 99m sulfur colloid. Each patient was anesthetized and the conjunctival melanoma was excised. SLNs were localized by a gamma probe, identified according to radioactivity and sentinel blue printing, and dissected, along with drainage of the associated lymphatic basins. The SLNs were evaluated by a pathologist using hematoxylin-eosin staining following serial sectioning and immunohistochemistry using a triple melanoma cocktail (S-100, Melan-A, and HMB-45 antigens).Results: Two SLNs were stained in the jugular chain during preoperative lymphoscintigraphy in the first patient, two SLNs were identified in the preauricular and submandibular areas in the second patient, and two SLNs were identified in the submandibular and parotid areas in the third patient. All lymph nodes identified by lymphoscintigraphy were dissected and identified at surgery with 100% accuracy in all three patients. All SLNs were histologically and immunohistochemically negative. Patients had good cosmetic and functional results, and maintained their visual acuity and ocular motility.Conclusion: Patients with conjunctival melanoma can undergo preoperative lymphoscintigraphy and SLN biopsy safely using radioactive technetium and isosulfan blue dye. Keywords: ocular melanoma, sentinel lymph node biopsy, lymphoscintigraphy, conjunctival melanomahttp://www.dovepress.com/sentinel-lymph-node-biopsy-for-conjunctival-malignant-melanoma-surgica-peer-reviewed-article-OPTH |
| spellingShingle | Wainstein AJA Drummond-Lage AP Kansaon MJM Bretas GO Almeida RF Gloria ALF Figueiredo ARP Sentinel lymph node biopsy for conjunctival malignant melanoma: surgical techniques Clinical Ophthalmology |
| title | Sentinel lymph node biopsy for conjunctival malignant melanoma: surgical techniques |
| title_full | Sentinel lymph node biopsy for conjunctival malignant melanoma: surgical techniques |
| title_fullStr | Sentinel lymph node biopsy for conjunctival malignant melanoma: surgical techniques |
| title_full_unstemmed | Sentinel lymph node biopsy for conjunctival malignant melanoma: surgical techniques |
| title_short | Sentinel lymph node biopsy for conjunctival malignant melanoma: surgical techniques |
| title_sort | sentinel lymph node biopsy for conjunctival malignant melanoma surgical techniques |
| url | http://www.dovepress.com/sentinel-lymph-node-biopsy-for-conjunctival-malignant-melanoma-surgica-peer-reviewed-article-OPTH |
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