Hydroxyapatite–Silicon Scaffold Promotes Osteogenic Differentiation of CGF Primary Cells
The application of scaffolding materials together with stem cell technologies plays a key role in tissue regeneration. Therefore, in this study, CGF (concentrated growth factor), which represents an autologous and biocompatible blood-derived product rich in growth factors and multipotent stem cells,...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/12/4/528 |
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| author | Laura Giannotti Benedetta Di Chiara Stanca Paola Nitti Francesco Spedicato Fabrizio Damiano Christian Demitri Nadia Calabriso Maria Annunziata Carluccio Andrea Palermo Franco Ferrante Luisa Siculella Eleonora Stanca |
| author_facet | Laura Giannotti Benedetta Di Chiara Stanca Paola Nitti Francesco Spedicato Fabrizio Damiano Christian Demitri Nadia Calabriso Maria Annunziata Carluccio Andrea Palermo Franco Ferrante Luisa Siculella Eleonora Stanca |
| author_sort | Laura Giannotti |
| collection | DOAJ |
| description | The application of scaffolding materials together with stem cell technologies plays a key role in tissue regeneration. Therefore, in this study, CGF (concentrated growth factor), which represents an autologous and biocompatible blood-derived product rich in growth factors and multipotent stem cells, was used together with a hydroxyapatite and silicon (HA-Si) scaffold, which represents a very interesting material in the field of bone reconstructive surgery. The aim of this work was to evaluate the potential osteogenic differentiation of CGF primary cells induced by HA-Si scaffolds. The cellular viability of CGF primary cells cultured on HA-Si scaffolds and their structural characterization were performed by MTT assay and SEM analysis, respectively. Moreover, the matrix mineralization of CGF primary cells on the HA-Si scaffold was evaluated through Alizarin red staining. The expression of osteogenic differentiation markers was investigated through mRNA quantification by real-time PCR. We found that the HA-Si scaffold was not cytotoxic for CGF primary cells, allowing their growth and proliferation. Furthermore, the HA-Si scaffold was able to induce increased levels of osteogenic markers, decreased levels of stemness markers in these cells, and the formation of a mineralized matrix. In conclusion, our results suggest that HA-Si scaffolds can be used as a biomaterial support for CGF application in the field of tissue regeneration. |
| first_indexed | 2024-03-11T05:14:13Z |
| format | Article |
| id | doaj.art-9dc53a14fe724d6291f654609bd4ee89 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-11T05:14:13Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-9dc53a14fe724d6291f654609bd4ee892023-11-17T18:23:32ZengMDPI AGBiology2079-77372023-03-0112452810.3390/biology12040528Hydroxyapatite–Silicon Scaffold Promotes Osteogenic Differentiation of CGF Primary CellsLaura Giannotti0Benedetta Di Chiara Stanca1Paola Nitti2Francesco Spedicato3Fabrizio Damiano4Christian Demitri5Nadia Calabriso6Maria Annunziata Carluccio7Andrea Palermo8Franco Ferrante9Luisa Siculella10Eleonora Stanca11Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, ItalyDepartment of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, ItalyDepartment of Engineering for Innovation, Campus Ecotekne, University of Salento, Via per Monteroni, 73100 Lecce, ItalyDepartment of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, ItalyDepartment of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, ItalyDepartment of Engineering for Innovation, Campus Ecotekne, University of Salento, Via per Monteroni, 73100 Lecce, ItalyNational Research Council (CNR) Institute of Clinical Physiology (IFC), 73100 Lecce, ItalyNational Research Council (CNR) Institute of Clinical Physiology (IFC), 73100 Lecce, ItalyImplant Dentistry College of Medicine and Dentistry, Birmingham B4 6BN, UKSpecialist in Oral Surgery, Private Practitioner, 73100 Lecce, ItalyDepartment of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, ItalyDepartment of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, ItalyThe application of scaffolding materials together with stem cell technologies plays a key role in tissue regeneration. Therefore, in this study, CGF (concentrated growth factor), which represents an autologous and biocompatible blood-derived product rich in growth factors and multipotent stem cells, was used together with a hydroxyapatite and silicon (HA-Si) scaffold, which represents a very interesting material in the field of bone reconstructive surgery. The aim of this work was to evaluate the potential osteogenic differentiation of CGF primary cells induced by HA-Si scaffolds. The cellular viability of CGF primary cells cultured on HA-Si scaffolds and their structural characterization were performed by MTT assay and SEM analysis, respectively. Moreover, the matrix mineralization of CGF primary cells on the HA-Si scaffold was evaluated through Alizarin red staining. The expression of osteogenic differentiation markers was investigated through mRNA quantification by real-time PCR. We found that the HA-Si scaffold was not cytotoxic for CGF primary cells, allowing their growth and proliferation. Furthermore, the HA-Si scaffold was able to induce increased levels of osteogenic markers, decreased levels of stemness markers in these cells, and the formation of a mineralized matrix. In conclusion, our results suggest that HA-Si scaffolds can be used as a biomaterial support for CGF application in the field of tissue regeneration.https://www.mdpi.com/2079-7737/12/4/528blood-derived biomaterialsCGFgrowth factorsosteogenic differentiationhydroxyapatite–silicon scaffoldtissue regeneration |
| spellingShingle | Laura Giannotti Benedetta Di Chiara Stanca Paola Nitti Francesco Spedicato Fabrizio Damiano Christian Demitri Nadia Calabriso Maria Annunziata Carluccio Andrea Palermo Franco Ferrante Luisa Siculella Eleonora Stanca Hydroxyapatite–Silicon Scaffold Promotes Osteogenic Differentiation of CGF Primary Cells Biology blood-derived biomaterials CGF growth factors osteogenic differentiation hydroxyapatite–silicon scaffold tissue regeneration |
| title | Hydroxyapatite–Silicon Scaffold Promotes Osteogenic Differentiation of CGF Primary Cells |
| title_full | Hydroxyapatite–Silicon Scaffold Promotes Osteogenic Differentiation of CGF Primary Cells |
| title_fullStr | Hydroxyapatite–Silicon Scaffold Promotes Osteogenic Differentiation of CGF Primary Cells |
| title_full_unstemmed | Hydroxyapatite–Silicon Scaffold Promotes Osteogenic Differentiation of CGF Primary Cells |
| title_short | Hydroxyapatite–Silicon Scaffold Promotes Osteogenic Differentiation of CGF Primary Cells |
| title_sort | hydroxyapatite silicon scaffold promotes osteogenic differentiation of cgf primary cells |
| topic | blood-derived biomaterials CGF growth factors osteogenic differentiation hydroxyapatite–silicon scaffold tissue regeneration |
| url | https://www.mdpi.com/2079-7737/12/4/528 |
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