<i>Oenanthe javanica</i> Ethanolic Extract Alleviates Inflammation and Modifies Gut Microbiota in Mice with DSS-Induced Colitis
<i>Oenanthe javanica</i>, commonly known as water dropwort, has long been used to treat acute and chronic hepatitis, abdominal pain, alcohol hangovers, and inflammation in various traditional medicine systems in Asia. However, whether <i>O. javanica</i> has beneficial effects...
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MDPI AG
2022-12-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/12/2429 |
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| author | Ui-Jin Bae Ha-Na Jang Sung-Hyen Lee Ji-Young Kim Gi-Chang Kim |
| author_facet | Ui-Jin Bae Ha-Na Jang Sung-Hyen Lee Ji-Young Kim Gi-Chang Kim |
| author_sort | Ui-Jin Bae |
| collection | DOAJ |
| description | <i>Oenanthe javanica</i>, commonly known as water dropwort, has long been used to treat acute and chronic hepatitis, abdominal pain, alcohol hangovers, and inflammation in various traditional medicine systems in Asia. However, whether <i>O. javanica</i> has beneficial effects on colitis-induced intestinal damage remains elusive. This study tested the hypothesis that <i>O. javanica</i> has anti-inflammatory and antioxidant activities in mice with dextran sulfate sodium (DSS)-induced colitis. First, treatment of <i>O. javanica</i> ethanol extract (OJE) inhibited the production of inflammatory cytokines in lipopolysaccharide-affected macrophages. Second, in mice with DSS-induced colitis, OJE administration reduced pathological damage to the colon while alleviating weight gain and decreasing colon length, including inflammation and mucosal necrosis. In addition, OJE significantly (<i>p</i> < 0.01) restricted the activation of nuclear factor-κB (NF-κB) and the secretion of pro-inflammatory mediators and increased the expression of Nrf2-phase 2 antioxidant enzymes. The results of 16S rRNA gene sequencing workflows for taxonomic assignment analysis confirmed that the diversity (richness and evenness) of fecal microbiota was markedly elevated in the OJE group. OJE administration reduced the abundance of <i>Proteobacteria</i> including <i>Escherichia</i> and increased the abundance of the genus <i>Muribaculum</i>. These results suggested that OJE exerts beneficial effects on inflammation and gut microbial composition in a mouse model of colitis. |
| first_indexed | 2024-03-09T17:23:13Z |
| format | Article |
| id | doaj.art-9dc6513148c14da7a1bbc02dc8840da3 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T17:23:13Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-9dc6513148c14da7a1bbc02dc8840da32023-11-24T12:57:56ZengMDPI AGAntioxidants2076-39212022-12-011112242910.3390/antiox11122429<i>Oenanthe javanica</i> Ethanolic Extract Alleviates Inflammation and Modifies Gut Microbiota in Mice with DSS-Induced ColitisUi-Jin Bae0Ha-Na Jang1Sung-Hyen Lee2Ji-Young Kim3Gi-Chang Kim4National Institute of Agricultural Sciences, Wanju 55365, Republic of KoreaNational Institute of Agricultural Sciences, Wanju 55365, Republic of KoreaNational Institute of Agricultural Sciences, Wanju 55365, Republic of KoreaNational Institute of Agricultural Sciences, Wanju 55365, Republic of KoreaNational Institute of Agricultural Sciences, Wanju 55365, Republic of Korea<i>Oenanthe javanica</i>, commonly known as water dropwort, has long been used to treat acute and chronic hepatitis, abdominal pain, alcohol hangovers, and inflammation in various traditional medicine systems in Asia. However, whether <i>O. javanica</i> has beneficial effects on colitis-induced intestinal damage remains elusive. This study tested the hypothesis that <i>O. javanica</i> has anti-inflammatory and antioxidant activities in mice with dextran sulfate sodium (DSS)-induced colitis. First, treatment of <i>O. javanica</i> ethanol extract (OJE) inhibited the production of inflammatory cytokines in lipopolysaccharide-affected macrophages. Second, in mice with DSS-induced colitis, OJE administration reduced pathological damage to the colon while alleviating weight gain and decreasing colon length, including inflammation and mucosal necrosis. In addition, OJE significantly (<i>p</i> < 0.01) restricted the activation of nuclear factor-κB (NF-κB) and the secretion of pro-inflammatory mediators and increased the expression of Nrf2-phase 2 antioxidant enzymes. The results of 16S rRNA gene sequencing workflows for taxonomic assignment analysis confirmed that the diversity (richness and evenness) of fecal microbiota was markedly elevated in the OJE group. OJE administration reduced the abundance of <i>Proteobacteria</i> including <i>Escherichia</i> and increased the abundance of the genus <i>Muribaculum</i>. These results suggested that OJE exerts beneficial effects on inflammation and gut microbial composition in a mouse model of colitis.https://www.mdpi.com/2076-3921/11/12/2429colitisinflammationoxidative stressmicrobiota<i>Oenanthe javanica</i>dextran sulfate sodium |
| spellingShingle | Ui-Jin Bae Ha-Na Jang Sung-Hyen Lee Ji-Young Kim Gi-Chang Kim <i>Oenanthe javanica</i> Ethanolic Extract Alleviates Inflammation and Modifies Gut Microbiota in Mice with DSS-Induced Colitis Antioxidants colitis inflammation oxidative stress microbiota <i>Oenanthe javanica</i> dextran sulfate sodium |
| title | <i>Oenanthe javanica</i> Ethanolic Extract Alleviates Inflammation and Modifies Gut Microbiota in Mice with DSS-Induced Colitis |
| title_full | <i>Oenanthe javanica</i> Ethanolic Extract Alleviates Inflammation and Modifies Gut Microbiota in Mice with DSS-Induced Colitis |
| title_fullStr | <i>Oenanthe javanica</i> Ethanolic Extract Alleviates Inflammation and Modifies Gut Microbiota in Mice with DSS-Induced Colitis |
| title_full_unstemmed | <i>Oenanthe javanica</i> Ethanolic Extract Alleviates Inflammation and Modifies Gut Microbiota in Mice with DSS-Induced Colitis |
| title_short | <i>Oenanthe javanica</i> Ethanolic Extract Alleviates Inflammation and Modifies Gut Microbiota in Mice with DSS-Induced Colitis |
| title_sort | i oenanthe javanica i ethanolic extract alleviates inflammation and modifies gut microbiota in mice with dss induced colitis |
| topic | colitis inflammation oxidative stress microbiota <i>Oenanthe javanica</i> dextran sulfate sodium |
| url | https://www.mdpi.com/2076-3921/11/12/2429 |
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