Effects of human parvovirus B19 VP1 unique region protein on macrophage responses
<p>Abstract</p> <p>Background</p> <p>Activity of secreted phospholipase A (sPLA2) has been implicated in a wide range of cellular responses. However, little is known about the function of human parvovirus B19-VP1 unique region (VP1u) with sPLA2 activity on macrophage.&l...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-01-01
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| Series: | Journal of Biomedical Science |
| Online Access: | http://www.jbiomedsci.com/content/16/1/13 |
| _version_ | 1811277885905829888 |
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| author | Lee Yi-Ju Tsai Chun-Chou Chiu Chun-Ching Tzang Bor-Show Lu I-Jung Shi Jing-Yu Hsu Tsai-Ching |
| author_facet | Lee Yi-Ju Tsai Chun-Chou Chiu Chun-Ching Tzang Bor-Show Lu I-Jung Shi Jing-Yu Hsu Tsai-Ching |
| author_sort | Lee Yi-Ju |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Activity of secreted phospholipase A (sPLA2) has been implicated in a wide range of cellular responses. However, little is known about the function of human parvovirus B19-VP1 unique region (VP1u) with sPLA2 activity on macrophage.</p> <p>Methods</p> <p>To investigate the roles of B19-VP1u in response to macrophage, phospholipase A2 activity, cell migration assay, phagocytosis activity, metalloproteinase assay, RT-PCR and immunoblotting were performed.</p> <p>Results</p> <p>In the present study, we report that migration, phagocytosis, IL-6, IL-1β mRNA, and MMP9 activity are significantly increased in RAW264.7 cells by B19-VP1u protein with sPLA2 activity, but not by B19-VP1uD175A protein that is mutated and lacks sPLA2 activity. Additionally, significant increases of phosphorylated ERK1/2 and JNK proteins were detected in macrophages that were treated with B19-VP1u protein, but not when they were treated with B19-VP1uD175A protein.</p> <p>Conclusion</p> <p>Taken together, our experimental results suggest that B19-VP1u with sPLA2 activity affects production of IL-6, IL-1β mRNA, and MMP9 activity, possibly through the involvement of ERK1/2 and JNK signaling pathways. These findings could provide clues in understanding the role of B19-VP1u and its sPLA2 enzymatic activity in B19 infection and B19-related diseases.</p> |
| first_indexed | 2024-04-13T00:24:39Z |
| format | Article |
| id | doaj.art-9dc67bff26454bf28462955f3de92a51 |
| institution | Directory Open Access Journal |
| issn | 1021-7770 1423-0127 |
| language | English |
| last_indexed | 2024-04-13T00:24:39Z |
| publishDate | 2009-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biomedical Science |
| spelling | doaj.art-9dc67bff26454bf28462955f3de92a512022-12-22T03:10:39ZengBMCJournal of Biomedical Science1021-77701423-01272009-01-011611310.1186/1423-0127-16-13Effects of human parvovirus B19 VP1 unique region protein on macrophage responsesLee Yi-JuTsai Chun-ChouChiu Chun-ChingTzang Bor-ShowLu I-JungShi Jing-YuHsu Tsai-Ching<p>Abstract</p> <p>Background</p> <p>Activity of secreted phospholipase A (sPLA2) has been implicated in a wide range of cellular responses. However, little is known about the function of human parvovirus B19-VP1 unique region (VP1u) with sPLA2 activity on macrophage.</p> <p>Methods</p> <p>To investigate the roles of B19-VP1u in response to macrophage, phospholipase A2 activity, cell migration assay, phagocytosis activity, metalloproteinase assay, RT-PCR and immunoblotting were performed.</p> <p>Results</p> <p>In the present study, we report that migration, phagocytosis, IL-6, IL-1β mRNA, and MMP9 activity are significantly increased in RAW264.7 cells by B19-VP1u protein with sPLA2 activity, but not by B19-VP1uD175A protein that is mutated and lacks sPLA2 activity. Additionally, significant increases of phosphorylated ERK1/2 and JNK proteins were detected in macrophages that were treated with B19-VP1u protein, but not when they were treated with B19-VP1uD175A protein.</p> <p>Conclusion</p> <p>Taken together, our experimental results suggest that B19-VP1u with sPLA2 activity affects production of IL-6, IL-1β mRNA, and MMP9 activity, possibly through the involvement of ERK1/2 and JNK signaling pathways. These findings could provide clues in understanding the role of B19-VP1u and its sPLA2 enzymatic activity in B19 infection and B19-related diseases.</p>http://www.jbiomedsci.com/content/16/1/13 |
| spellingShingle | Lee Yi-Ju Tsai Chun-Chou Chiu Chun-Ching Tzang Bor-Show Lu I-Jung Shi Jing-Yu Hsu Tsai-Ching Effects of human parvovirus B19 VP1 unique region protein on macrophage responses Journal of Biomedical Science |
| title | Effects of human parvovirus B19 VP1 unique region protein on macrophage responses |
| title_full | Effects of human parvovirus B19 VP1 unique region protein on macrophage responses |
| title_fullStr | Effects of human parvovirus B19 VP1 unique region protein on macrophage responses |
| title_full_unstemmed | Effects of human parvovirus B19 VP1 unique region protein on macrophage responses |
| title_short | Effects of human parvovirus B19 VP1 unique region protein on macrophage responses |
| title_sort | effects of human parvovirus b19 vp1 unique region protein on macrophage responses |
| url | http://www.jbiomedsci.com/content/16/1/13 |
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