Fibre Composition and Maturity of Forage-Based Diets Affects the Fluid Balance, Faecal Water-Holding Capacity and Microbial Ecosystem in French Trotters
Racing events challenge the fluid balance of athletic horses. The equine large intestine functions as a fluid reservoir, since the properties of dietary forage fibre affect the digesta water content and the milieu of this ecosystem. This study aimed to investigate the effect of grass maturity and le...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/13/3/328 |
| _version_ | 1797625344540278784 |
|---|---|
| author | Sara Muhonen Véronique Julliand |
| author_facet | Sara Muhonen Véronique Julliand |
| author_sort | Sara Muhonen |
| collection | DOAJ |
| description | Racing events challenge the fluid balance of athletic horses. The equine large intestine functions as a fluid reservoir, since the properties of dietary forage fibre affect the digesta water content and the milieu of this ecosystem. This study aimed to investigate the effect of grass maturity and legume forage on the faecal water-holding capacity (WHC) and microbial ecosystem, and the fluid balance and body weight (BW) of French trotters in race training. Six geldings were offered three diets with different fibre compositions: concentrate and late-harvested mature grass haylage (35:65 energy ratio) (CMGH); early-harvested grass haylage and mature grass haylage (80:20) (EGH); and lucerne and mature grass haylage (80:20) (LH), for 24 days in a Latin square design. Body weights were lower and faecal WHC higher when the horses were fed EGH compared to CMGH and LH (485 vs. 492 and 492 kg, <i>p</i> < 0.001; 12.6 vs. 11.1 and 11.4 g H<sub>2</sub>O/g dry faeces, <i>p</i> = 0.014, respectively). Total water intake and output did not differ between diets, but water excretion via faeces was lower and via urine was greater on EGH compared to CMGH and LH (13.1 vs. 18.8 and 17.6 kg, <i>p</i> = 0.001; 10.5 vs. 7.6 and 7.9 kg, <i>p</i> = 0.032, respectively). Total bacteria concentrations were higher on EGH than CMGH and LH (5.4 × 10<sup>11</sup> vs. 2.8 × 10<sup>11</sup> and 2.8 × 10<sup>11</sup> CFU/mL, <i>p</i> = 0.018, respectively). Concentrations of butyrate were greater, and pH was lower when fed EGH compared to CMGH and LH (3.0 vs. 2.0 and 1.6 mmol/L, <i>p</i> = 0.034; 6.5 vs. 6.9 and 7.2, <i>p</i> = 0.005, respectively). In conclusion, forage harvested at an early stage of maturity could benefit athletic horses’ fluid balance by providing a more available large intestine fluid reservoir without increasing BW. |
| first_indexed | 2024-03-11T09:55:14Z |
| format | Article |
| id | doaj.art-9dcb79452c9447359bb19eab14a92e18 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T09:55:14Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-9dcb79452c9447359bb19eab14a92e182023-11-16T15:59:21ZengMDPI AGAnimals2076-26152023-01-0113332810.3390/ani13030328Fibre Composition and Maturity of Forage-Based Diets Affects the Fluid Balance, Faecal Water-Holding Capacity and Microbial Ecosystem in French TrottersSara Muhonen0Véronique Julliand1L’institut Agro Dijon, Burgundy Franche-Comté University, PAM UMR A 02.102, 21000 Dijon, FranceL’institut Agro Dijon, Burgundy Franche-Comté University, PAM UMR A 02.102, 21000 Dijon, FranceRacing events challenge the fluid balance of athletic horses. The equine large intestine functions as a fluid reservoir, since the properties of dietary forage fibre affect the digesta water content and the milieu of this ecosystem. This study aimed to investigate the effect of grass maturity and legume forage on the faecal water-holding capacity (WHC) and microbial ecosystem, and the fluid balance and body weight (BW) of French trotters in race training. Six geldings were offered three diets with different fibre compositions: concentrate and late-harvested mature grass haylage (35:65 energy ratio) (CMGH); early-harvested grass haylage and mature grass haylage (80:20) (EGH); and lucerne and mature grass haylage (80:20) (LH), for 24 days in a Latin square design. Body weights were lower and faecal WHC higher when the horses were fed EGH compared to CMGH and LH (485 vs. 492 and 492 kg, <i>p</i> < 0.001; 12.6 vs. 11.1 and 11.4 g H<sub>2</sub>O/g dry faeces, <i>p</i> = 0.014, respectively). Total water intake and output did not differ between diets, but water excretion via faeces was lower and via urine was greater on EGH compared to CMGH and LH (13.1 vs. 18.8 and 17.6 kg, <i>p</i> = 0.001; 10.5 vs. 7.6 and 7.9 kg, <i>p</i> = 0.032, respectively). Total bacteria concentrations were higher on EGH than CMGH and LH (5.4 × 10<sup>11</sup> vs. 2.8 × 10<sup>11</sup> and 2.8 × 10<sup>11</sup> CFU/mL, <i>p</i> = 0.018, respectively). Concentrations of butyrate were greater, and pH was lower when fed EGH compared to CMGH and LH (3.0 vs. 2.0 and 1.6 mmol/L, <i>p</i> = 0.034; 6.5 vs. 6.9 and 7.2, <i>p</i> = 0.005, respectively). In conclusion, forage harvested at an early stage of maturity could benefit athletic horses’ fluid balance by providing a more available large intestine fluid reservoir without increasing BW.https://www.mdpi.com/2076-2615/13/3/328equinefaecal microbiotahaylagelucerne |
| spellingShingle | Sara Muhonen Véronique Julliand Fibre Composition and Maturity of Forage-Based Diets Affects the Fluid Balance, Faecal Water-Holding Capacity and Microbial Ecosystem in French Trotters Animals equine faecal microbiota haylage lucerne |
| title | Fibre Composition and Maturity of Forage-Based Diets Affects the Fluid Balance, Faecal Water-Holding Capacity and Microbial Ecosystem in French Trotters |
| title_full | Fibre Composition and Maturity of Forage-Based Diets Affects the Fluid Balance, Faecal Water-Holding Capacity and Microbial Ecosystem in French Trotters |
| title_fullStr | Fibre Composition and Maturity of Forage-Based Diets Affects the Fluid Balance, Faecal Water-Holding Capacity and Microbial Ecosystem in French Trotters |
| title_full_unstemmed | Fibre Composition and Maturity of Forage-Based Diets Affects the Fluid Balance, Faecal Water-Holding Capacity and Microbial Ecosystem in French Trotters |
| title_short | Fibre Composition and Maturity of Forage-Based Diets Affects the Fluid Balance, Faecal Water-Holding Capacity and Microbial Ecosystem in French Trotters |
| title_sort | fibre composition and maturity of forage based diets affects the fluid balance faecal water holding capacity and microbial ecosystem in french trotters |
| topic | equine faecal microbiota haylage lucerne |
| url | https://www.mdpi.com/2076-2615/13/3/328 |
| work_keys_str_mv | AT saramuhonen fibrecompositionandmaturityofforagebaseddietsaffectsthefluidbalancefaecalwaterholdingcapacityandmicrobialecosysteminfrenchtrotters AT veroniquejulliand fibrecompositionandmaturityofforagebaseddietsaffectsthefluidbalancefaecalwaterholdingcapacityandmicrobialecosysteminfrenchtrotters |