Preservation of semen from Kintamani Bali dogs by freezing method
Objective: To explore the effect of glycerol at different concentrations using different extenders on DNA fragmentation and motility of frozen-thawed Kintamani Bali dog spermatozoa. Materials and Methods: Sample was collected from four mature Kintamani Bali dogs. Each ejacu¬late was prepared for cr...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Network for the Veterinarians of Bangladesh
2019-06-01
|
| Series: | Journal of Advanced Veterinary and Animal Research |
| Subjects: | |
| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=18171 |
| _version_ | 1818174766506311680 |
|---|---|
| author | I Ketut Puja Ni Made Sawitri Nisa Maharani Luh Gde Sri Surya Heryani Anak Agung Gde Oka Dharmayudha I Wayan Nico Fajar Gunawan |
| author_facet | I Ketut Puja Ni Made Sawitri Nisa Maharani Luh Gde Sri Surya Heryani Anak Agung Gde Oka Dharmayudha I Wayan Nico Fajar Gunawan |
| author_sort | I Ketut Puja |
| collection | DOAJ |
| description | Objective: To explore the effect of glycerol at different concentrations using different extenders on DNA fragmentation and motility of frozen-thawed Kintamani Bali dog spermatozoa.
Materials and Methods: Sample was collected from four mature Kintamani Bali dogs. Each ejacu¬late was prepared for cryopreservation with two different semen extenders; egg yolk Tris extender and coconut water-based extender. For each extender, three different glycerol concentrations were used; 4%, 6%, and 8%. Each of the six aliquots was loaded into 0.5 ml cryotube, placed on a styrofoam box 5 cm over liquid nitrogen for 10 min, and immersed in liquid nitrogen up to 8 min. Then, the frozen cryotubes were transferred into liquid nitrogen container. The cryotubes were thawed in a water bath at 38.5°C for 120 sec. After equilibration and thawing, each sample was assessed for motility parameters and for DNA fragmentation.
Results: The addition of 6% glycerol to extenders revealed the most effective addition of glycerol on motility and sperm DNA fragmentation after equilibrium and post-thawing.
Conclusion: It is concluded that both extenders with the addition of 6% glycerol are safe to be used as an extender in Kintamani Bali dog semen preservation, and DNA fragmentation of Kintamani Bali dog spermatozoa was not influenced by the freezing procedure. [J Adv Vet Anim Res 2019; 6(2.000): 158-162] |
| first_indexed | 2024-12-11T19:49:37Z |
| format | Article |
| id | doaj.art-9dcc082919294461a984f69df4a47480 |
| institution | Directory Open Access Journal |
| issn | 2311-7710 |
| language | English |
| last_indexed | 2024-12-11T19:49:37Z |
| publishDate | 2019-06-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj.art-9dcc082919294461a984f69df4a474802022-12-22T00:52:49ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102019-06-016215816210.5455/javar.2019.f32618171Preservation of semen from Kintamani Bali dogs by freezing methodI Ketut Puja0Ni Made Sawitri1Nisa Maharani2Luh Gde Sri Surya Heryani3Anak Agung Gde Oka Dharmayudha4I Wayan Nico Fajar Gunawan5Veterinary Genetics and Reproduction Technology Laboratory, Faculty of Veterinary Medicine, Udayana University, Bali, Indonesia Veterinary Genetics and Reproduction Technology Laboratory, Faculty of Veterinary Medicine, Udayana University, Bali, Indonesia Veterinary Genetics and Reproduction Technology Laboratory, Faculty of Veterinary Medicine, Udayana University, Bali, Indonesia Laboratory of Veterinary Anatomy, Faculty of Veterinary Medicine, Udayana University, Bali, lndonesia Veterinary Surgery and Radiology Laboratory, Faculty of Veterinary Medicine, Udayana University, Bali, Indonesia Veterinary Surgery and Radiology Laboratory, Faculty of Veterinary Medicine, Udayana University, Bali, Indonesia.Objective: To explore the effect of glycerol at different concentrations using different extenders on DNA fragmentation and motility of frozen-thawed Kintamani Bali dog spermatozoa. Materials and Methods: Sample was collected from four mature Kintamani Bali dogs. Each ejacu¬late was prepared for cryopreservation with two different semen extenders; egg yolk Tris extender and coconut water-based extender. For each extender, three different glycerol concentrations were used; 4%, 6%, and 8%. Each of the six aliquots was loaded into 0.5 ml cryotube, placed on a styrofoam box 5 cm over liquid nitrogen for 10 min, and immersed in liquid nitrogen up to 8 min. Then, the frozen cryotubes were transferred into liquid nitrogen container. The cryotubes were thawed in a water bath at 38.5°C for 120 sec. After equilibration and thawing, each sample was assessed for motility parameters and for DNA fragmentation. Results: The addition of 6% glycerol to extenders revealed the most effective addition of glycerol on motility and sperm DNA fragmentation after equilibrium and post-thawing. Conclusion: It is concluded that both extenders with the addition of 6% glycerol are safe to be used as an extender in Kintamani Bali dog semen preservation, and DNA fragmentation of Kintamani Bali dog spermatozoa was not influenced by the freezing procedure. [J Adv Vet Anim Res 2019; 6(2.000): 158-162]http://www.ejmanager.com/fulltextpdf.php?mno=18171Cryopreservation; DNA fragmentation; Kintamani Bali dog; motility |
| spellingShingle | I Ketut Puja Ni Made Sawitri Nisa Maharani Luh Gde Sri Surya Heryani Anak Agung Gde Oka Dharmayudha I Wayan Nico Fajar Gunawan Preservation of semen from Kintamani Bali dogs by freezing method Journal of Advanced Veterinary and Animal Research Cryopreservation; DNA fragmentation; Kintamani Bali dog; motility |
| title | Preservation of semen from Kintamani Bali dogs by freezing method |
| title_full | Preservation of semen from Kintamani Bali dogs by freezing method |
| title_fullStr | Preservation of semen from Kintamani Bali dogs by freezing method |
| title_full_unstemmed | Preservation of semen from Kintamani Bali dogs by freezing method |
| title_short | Preservation of semen from Kintamani Bali dogs by freezing method |
| title_sort | preservation of semen from kintamani bali dogs by freezing method |
| topic | Cryopreservation; DNA fragmentation; Kintamani Bali dog; motility |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=18171 |
| work_keys_str_mv | AT iketutpuja preservationofsemenfromkintamanibalidogsbyfreezingmethod AT nimadesawitri preservationofsemenfromkintamanibalidogsbyfreezingmethod AT nisamaharani preservationofsemenfromkintamanibalidogsbyfreezingmethod AT luhgdesrisuryaheryani preservationofsemenfromkintamanibalidogsbyfreezingmethod AT anakagunggdeokadharmayudha preservationofsemenfromkintamanibalidogsbyfreezingmethod AT iwayannicofajargunawan preservationofsemenfromkintamanibalidogsbyfreezingmethod |