Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss
Bryophytes are the dominant vegetation in the Antarctic continent. They have suffered more unpleasant ultraviolet radiation due to the Antarctic ozone layer destruction. However, it remains unclear about the molecular mechanism of Antarctic moss acclimation to UV-B light. Here, the transcriptomics a...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.788377/full |
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| author | Shenghao Liu Shenghao Liu Shuo Fang Chenlin Liu Linlin Zhao Linlin Zhao Bailin Cong Zhaohui Zhang Zhaohui Zhang |
| author_facet | Shenghao Liu Shenghao Liu Shuo Fang Chenlin Liu Linlin Zhao Linlin Zhao Bailin Cong Zhaohui Zhang Zhaohui Zhang |
| author_sort | Shenghao Liu |
| collection | DOAJ |
| description | Bryophytes are the dominant vegetation in the Antarctic continent. They have suffered more unpleasant ultraviolet radiation due to the Antarctic ozone layer destruction. However, it remains unclear about the molecular mechanism of Antarctic moss acclimation to UV-B light. Here, the transcriptomics and metabolomics approaches were conducted to uncover transcriptional and metabolic profiling of the Antarctic moss Leptobryum pyriforme under UV-B radiation. Totally, 67,290 unigenes with N50 length of 2,055 bp were assembled. Of them, 1,594 unigenes were significantly up-regulated and 3353 unigenes were markedly down-regulated under UV-B radiation. These differentially expressed genes (DEGs) involved in UV-B signaling, flavonoid biosynthesis, ROS scavenging, and DNA repair. In addition, a total of 531 metabolites were detected, while flavonoids and anthocyanins accounted for 10.36% of the total compounds. There were 49 upregulated metabolites and 41 downregulated metabolites under UV-B radiation. Flavonoids were the most significantly changed metabolites. qPCR analysis showed that UVR8-COP1-HY5 signaling pathway genes and photolyase genes (i.e., LpUVR3, LpPHR1, and LpDPL) were significantly up-regulated under UV-B light. In addition, the expression levels of JA signaling pathway-related genes (i.e., OPR and JAZ) and flavonoid biosynthesis-related genes were also significantly increased under UV-B radiation. The integrative data analysis showed that UVR8-mediated signaling, jasmonate signaling, flavonoid biosynthesis pathway and DNA repair system might contribute to L. pyriforme acclimating to UV-B radiation. Therefore, these findings present a novel knowledge for understanding the adaption of Antarctic moss to polar environments and provide a foundation for assessing the impact of global climate change on Antarctic land plants. |
| first_indexed | 2024-12-14T13:56:35Z |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-14T13:56:35Z |
| publishDate | 2021-12-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-9dd413fed743441aa9660e4d60efe4532022-12-21T22:58:50ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-12-011210.3389/fpls.2021.788377788377Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic MossShenghao Liu0Shenghao Liu1Shuo Fang2Chenlin Liu3Linlin Zhao4Linlin Zhao5Bailin Cong6Zhaohui Zhang7Zhaohui Zhang8Key Laboratory of Marine Ecology and Environment Science, First Institute of Oceanography, Natural Resources Ministry, Qingdao, ChinaMarine Ecology and Environmental Science Laboratory, Pilot National Laboratory for Marine Science and Technology, Qingdao, ChinaKey Laboratory of Marine Ecology and Environment Science, First Institute of Oceanography, Natural Resources Ministry, Qingdao, ChinaKey Laboratory of Marine Ecology and Environment Science, First Institute of Oceanography, Natural Resources Ministry, Qingdao, ChinaKey Laboratory of Marine Ecology and Environment Science, First Institute of Oceanography, Natural Resources Ministry, Qingdao, ChinaMarine Ecology and Environmental Science Laboratory, Pilot National Laboratory for Marine Science and Technology, Qingdao, ChinaKey Laboratory of Marine Ecology and Environment Science, First Institute of Oceanography, Natural Resources Ministry, Qingdao, ChinaKey Laboratory of Marine Ecology and Environment Science, First Institute of Oceanography, Natural Resources Ministry, Qingdao, ChinaMarine Ecology and Environmental Science Laboratory, Pilot National Laboratory for Marine Science and Technology, Qingdao, ChinaBryophytes are the dominant vegetation in the Antarctic continent. They have suffered more unpleasant ultraviolet radiation due to the Antarctic ozone layer destruction. However, it remains unclear about the molecular mechanism of Antarctic moss acclimation to UV-B light. Here, the transcriptomics and metabolomics approaches were conducted to uncover transcriptional and metabolic profiling of the Antarctic moss Leptobryum pyriforme under UV-B radiation. Totally, 67,290 unigenes with N50 length of 2,055 bp were assembled. Of them, 1,594 unigenes were significantly up-regulated and 3353 unigenes were markedly down-regulated under UV-B radiation. These differentially expressed genes (DEGs) involved in UV-B signaling, flavonoid biosynthesis, ROS scavenging, and DNA repair. In addition, a total of 531 metabolites were detected, while flavonoids and anthocyanins accounted for 10.36% of the total compounds. There were 49 upregulated metabolites and 41 downregulated metabolites under UV-B radiation. Flavonoids were the most significantly changed metabolites. qPCR analysis showed that UVR8-COP1-HY5 signaling pathway genes and photolyase genes (i.e., LpUVR3, LpPHR1, and LpDPL) were significantly up-regulated under UV-B light. In addition, the expression levels of JA signaling pathway-related genes (i.e., OPR and JAZ) and flavonoid biosynthesis-related genes were also significantly increased under UV-B radiation. The integrative data analysis showed that UVR8-mediated signaling, jasmonate signaling, flavonoid biosynthesis pathway and DNA repair system might contribute to L. pyriforme acclimating to UV-B radiation. Therefore, these findings present a novel knowledge for understanding the adaption of Antarctic moss to polar environments and provide a foundation for assessing the impact of global climate change on Antarctic land plants.https://www.frontiersin.org/articles/10.3389/fpls.2021.788377/fullabiotic stressbryophytesflavonoidsmetabolometranscriptomeultraviolet-B radiation |
| spellingShingle | Shenghao Liu Shenghao Liu Shuo Fang Chenlin Liu Linlin Zhao Linlin Zhao Bailin Cong Zhaohui Zhang Zhaohui Zhang Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss Frontiers in Plant Science abiotic stress bryophytes flavonoids metabolome transcriptome ultraviolet-B radiation |
| title | Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss |
| title_full | Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss |
| title_fullStr | Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss |
| title_full_unstemmed | Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss |
| title_short | Transcriptomics Integrated With Metabolomics Reveal the Effects of Ultraviolet-B Radiation on Flavonoid Biosynthesis in Antarctic Moss |
| title_sort | transcriptomics integrated with metabolomics reveal the effects of ultraviolet b radiation on flavonoid biosynthesis in antarctic moss |
| topic | abiotic stress bryophytes flavonoids metabolome transcriptome ultraviolet-B radiation |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.788377/full |
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