Ovarian Decellularized Bioscaffolds Provide an Optimal Microenvironment for Cell Growth and Differentiation In Vitro
Ovarian failure is the most common cause of infertility. Although numerous strategies have been proposed, a definitive solution for recovering ovarian functions and restoring fertility is currently unavailable. One innovative alternative may be represented by the development of an “artificial ovary”...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/10/8/2126 |
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| author | Georgia Pennarossa Teresina De Iorio Fulvio Gandolfi Tiziana A. L. Brevini |
| author_facet | Georgia Pennarossa Teresina De Iorio Fulvio Gandolfi Tiziana A. L. Brevini |
| author_sort | Georgia Pennarossa |
| collection | DOAJ |
| description | Ovarian failure is the most common cause of infertility. Although numerous strategies have been proposed, a definitive solution for recovering ovarian functions and restoring fertility is currently unavailable. One innovative alternative may be represented by the development of an “artificial ovary” that could be transplanted in patients for re-establishing reproductive activities. Here, we describe a novel approach for successful repopulation of decellularized ovarian bioscaffolds in vitro. Porcine whole ovaries were subjected to a decellularization protocol that removed the cell compartment, while maintaining the macrostructure and microstructure of the original tissue. The obtained bioscaffolds were then repopulated with porcine ovarian cells or with epigenetically erased porcine and human dermal fibroblasts. The results obtained demonstrated that the decellularized extracellular matrix (ECM)-based scaffold may constitute a suitable niche for ex vivo culture of ovarian cells. Furthermore, it was able to properly drive epigenetically erased cell differentiation, fate, and viability. Overall, the method described represents a powerful tool for the in vitro creation of a bioengineered ovary that may constitute a promising solution for hormone and fertility restoration. In addition, it allows for the creation of a suitable 3D platform with useful applications both in toxicological and transplantation studies. |
| first_indexed | 2024-03-10T08:55:43Z |
| format | Article |
| id | doaj.art-9dd522ccec104f64aed93d4205ba0896 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T08:55:43Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-9dd522ccec104f64aed93d4205ba08962023-11-22T07:12:07ZengMDPI AGCells2073-44092021-08-01108212610.3390/cells10082126Ovarian Decellularized Bioscaffolds Provide an Optimal Microenvironment for Cell Growth and Differentiation In VitroGeorgia Pennarossa0Teresina De Iorio1Fulvio Gandolfi2Tiziana A. L. Brevini3Laboratory of Biomedical Embryology, Department of Health, Animal Science and Food Safety and Center for Stem Cell Research, Università Degli Studi di Milano, 20133 Milan, ItalyLaboratory of Biomedical Embryology, Department of Health, Animal Science and Food Safety and Center for Stem Cell Research, Università Degli Studi di Milano, 20133 Milan, ItalyLaboratory of Biomedical Embryology, Department of Agricultural and Environmental Sciences—Production, Landscape, Agroenergy, Università Degli Studi di Milano, 20133 Milan, ItalyLaboratory of Biomedical Embryology, Department of Health, Animal Science and Food Safety and Center for Stem Cell Research, Università Degli Studi di Milano, 20133 Milan, ItalyOvarian failure is the most common cause of infertility. Although numerous strategies have been proposed, a definitive solution for recovering ovarian functions and restoring fertility is currently unavailable. One innovative alternative may be represented by the development of an “artificial ovary” that could be transplanted in patients for re-establishing reproductive activities. Here, we describe a novel approach for successful repopulation of decellularized ovarian bioscaffolds in vitro. Porcine whole ovaries were subjected to a decellularization protocol that removed the cell compartment, while maintaining the macrostructure and microstructure of the original tissue. The obtained bioscaffolds were then repopulated with porcine ovarian cells or with epigenetically erased porcine and human dermal fibroblasts. The results obtained demonstrated that the decellularized extracellular matrix (ECM)-based scaffold may constitute a suitable niche for ex vivo culture of ovarian cells. Furthermore, it was able to properly drive epigenetically erased cell differentiation, fate, and viability. Overall, the method described represents a powerful tool for the in vitro creation of a bioengineered ovary that may constitute a promising solution for hormone and fertility restoration. In addition, it allows for the creation of a suitable 3D platform with useful applications both in toxicological and transplantation studies.https://www.mdpi.com/2073-4409/10/8/2126whole-ovary decellularizationECM-based scaffold repopulationepigenetically erased cellsporcinehumanfibroblasts |
| spellingShingle | Georgia Pennarossa Teresina De Iorio Fulvio Gandolfi Tiziana A. L. Brevini Ovarian Decellularized Bioscaffolds Provide an Optimal Microenvironment for Cell Growth and Differentiation In Vitro Cells whole-ovary decellularization ECM-based scaffold repopulation epigenetically erased cells porcine human fibroblasts |
| title | Ovarian Decellularized Bioscaffolds Provide an Optimal Microenvironment for Cell Growth and Differentiation In Vitro |
| title_full | Ovarian Decellularized Bioscaffolds Provide an Optimal Microenvironment for Cell Growth and Differentiation In Vitro |
| title_fullStr | Ovarian Decellularized Bioscaffolds Provide an Optimal Microenvironment for Cell Growth and Differentiation In Vitro |
| title_full_unstemmed | Ovarian Decellularized Bioscaffolds Provide an Optimal Microenvironment for Cell Growth and Differentiation In Vitro |
| title_short | Ovarian Decellularized Bioscaffolds Provide an Optimal Microenvironment for Cell Growth and Differentiation In Vitro |
| title_sort | ovarian decellularized bioscaffolds provide an optimal microenvironment for cell growth and differentiation in vitro |
| topic | whole-ovary decellularization ECM-based scaffold repopulation epigenetically erased cells porcine human fibroblasts |
| url | https://www.mdpi.com/2073-4409/10/8/2126 |
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