Allergen Content of Therapeutic Preparations for Allergen-Specific Immunotherapy of European Paper Wasp Venom Allergy
Allergy to <i>Polistes dominula</i> (European paper wasp) venom is of particular relevance in Southern Europe, potentially becoming a threat in other regions in the near future, and can be effectively cured by venom immunotherapy (VIT). As allergen content in extracts may vary and have a...
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| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/14/4/284 |
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| author | Johannes Grosch Antoine Lesur Stéphanie Kler François Bernardin Gunnar Dittmar Elisabetta Francescato Simon J. Hewings Constanze A. Jakwerth Ulrich M. Zissler Matthew D. Heath Markus Ollert Matthias F. Kramer Christiane Hilger Maria Beatrice Bilò Carsten B. Schmidt-Weber Simon Blank |
| author_facet | Johannes Grosch Antoine Lesur Stéphanie Kler François Bernardin Gunnar Dittmar Elisabetta Francescato Simon J. Hewings Constanze A. Jakwerth Ulrich M. Zissler Matthew D. Heath Markus Ollert Matthias F. Kramer Christiane Hilger Maria Beatrice Bilò Carsten B. Schmidt-Weber Simon Blank |
| author_sort | Johannes Grosch |
| collection | DOAJ |
| description | Allergy to <i>Polistes dominula</i> (European paper wasp) venom is of particular relevance in Southern Europe, potentially becoming a threat in other regions in the near future, and can be effectively cured by venom immunotherapy (VIT). As allergen content in extracts may vary and have an impact on diagnostic and therapeutic approaches, the aim was to compare five therapeutic preparations for VIT of <i>P. dominula</i> venom allergy available in Spain. Products from five different suppliers were analyzed by SDS-PAGE and LC-MS/MS and compared with a reference venom sample. Three products with <i>P. dominula</i> venom and one product with a venom mixture of American <i>Polistes</i> species showed a comparable band pattern in SDS-PAGE as the reference sample and the bands of the major allergens phospholipase A1 and antigen 5 were assignable. The other product, which consists of a mixture of American <i>Polistes</i> species, exhibited the typical band pattern in one, but not in another sample from a second batch. All annotated <i>P. dominula</i> allergens were detected at comparable levels in LC-MS/MS analysis of products containing <i>P. dominula</i> venom. Due to a lack of genomic information on the American <i>Polistes</i> species, the remaining products were not analyzed by this method. The major <i>Polistes</i> allergens were present in comparable amounts in the majority, but not in all investigated samples of venom preparations for VIT of <i>P. dominula</i> venom allergy. |
| first_indexed | 2024-03-09T04:09:51Z |
| format | Article |
| id | doaj.art-9dd7396232544d4c823462a5d47153c8 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T04:09:51Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-9dd7396232544d4c823462a5d47153c82023-12-03T14:02:04ZengMDPI AGToxins2072-66512022-04-0114428410.3390/toxins14040284Allergen Content of Therapeutic Preparations for Allergen-Specific Immunotherapy of European Paper Wasp Venom AllergyJohannes Grosch0Antoine Lesur1Stéphanie Kler2François Bernardin3Gunnar Dittmar4Elisabetta Francescato5Simon J. Hewings6Constanze A. Jakwerth7Ulrich M. Zissler8Matthew D. Heath9Markus Ollert10Matthias F. Kramer11Christiane Hilger12Maria Beatrice Bilò13Carsten B. Schmidt-Weber14Simon Blank15Center of Allergy and Environment (ZAUM), Technical University of Munich, School of Medicine and Helmholtz Center Munich, German Research Center for Environmental Health, Member of the German Center of Lung Research (DZL), Member of the Immunology and Inflammation Initiative of the Helmholtz Association, 85764 Munich, GermanyQuantitative Biology Unit, Luxembourg Institute of Health (LIH), 1445 Strassen, LuxembourgDepartment of Infection and Immunity, Luxembourg Institute of Health (LIH), 4354 Esch-Sur-Alzette, LuxembourgQuantitative Biology Unit, Luxembourg Institute of Health (LIH), 1445 Strassen, LuxembourgQuantitative Biology Unit, Luxembourg Institute of Health (LIH), 1445 Strassen, LuxembourgEntomon SRL, 50012 Florence, ItalyAllergy Therapeutics PLC, Worthing BN14 8SA, UKCenter of Allergy and Environment (ZAUM), Technical University of Munich, School of Medicine and Helmholtz Center Munich, German Research Center for Environmental Health, Member of the German Center of Lung Research (DZL), Member of the Immunology and Inflammation Initiative of the Helmholtz Association, 85764 Munich, GermanyCenter of Allergy and Environment (ZAUM), Technical University of Munich, School of Medicine and Helmholtz Center Munich, German Research Center for Environmental Health, Member of the German Center of Lung Research (DZL), Member of the Immunology and Inflammation Initiative of the Helmholtz Association, 85764 Munich, GermanyAllergy Therapeutics PLC, Worthing BN14 8SA, UKDepartment of Infection and Immunity, Luxembourg Institute of Health (LIH), 4354 Esch-Sur-Alzette, LuxembourgAllergy Therapeutics PLC, Worthing BN14 8SA, UKDepartment of Infection and Immunity, Luxembourg Institute of Health (LIH), 4354 Esch-Sur-Alzette, LuxembourgDepartment of Clinical and Molecular Sciences, Polytechnic University of Marche, Ancona and Allergy Unit, Department of Internal Medicine, University Hospital of Ancona, 60121 Ancona, ItalyCenter of Allergy and Environment (ZAUM), Technical University of Munich, School of Medicine and Helmholtz Center Munich, German Research Center for Environmental Health, Member of the German Center of Lung Research (DZL), Member of the Immunology and Inflammation Initiative of the Helmholtz Association, 85764 Munich, GermanyCenter of Allergy and Environment (ZAUM), Technical University of Munich, School of Medicine and Helmholtz Center Munich, German Research Center for Environmental Health, Member of the German Center of Lung Research (DZL), Member of the Immunology and Inflammation Initiative of the Helmholtz Association, 85764 Munich, GermanyAllergy to <i>Polistes dominula</i> (European paper wasp) venom is of particular relevance in Southern Europe, potentially becoming a threat in other regions in the near future, and can be effectively cured by venom immunotherapy (VIT). As allergen content in extracts may vary and have an impact on diagnostic and therapeutic approaches, the aim was to compare five therapeutic preparations for VIT of <i>P. dominula</i> venom allergy available in Spain. Products from five different suppliers were analyzed by SDS-PAGE and LC-MS/MS and compared with a reference venom sample. Three products with <i>P. dominula</i> venom and one product with a venom mixture of American <i>Polistes</i> species showed a comparable band pattern in SDS-PAGE as the reference sample and the bands of the major allergens phospholipase A1 and antigen 5 were assignable. The other product, which consists of a mixture of American <i>Polistes</i> species, exhibited the typical band pattern in one, but not in another sample from a second batch. All annotated <i>P. dominula</i> allergens were detected at comparable levels in LC-MS/MS analysis of products containing <i>P. dominula</i> venom. Due to a lack of genomic information on the American <i>Polistes</i> species, the remaining products were not analyzed by this method. The major <i>Polistes</i> allergens were present in comparable amounts in the majority, but not in all investigated samples of venom preparations for VIT of <i>P. dominula</i> venom allergy.https://www.mdpi.com/2072-6651/14/4/284allergen-specific immunotherapyAmerican <i>Polistes</i> speciesEuropean paper waspEuropean <i>Polistes</i> speciesHymenoptera venom allergy<i>Polistes dominula</i> |
| spellingShingle | Johannes Grosch Antoine Lesur Stéphanie Kler François Bernardin Gunnar Dittmar Elisabetta Francescato Simon J. Hewings Constanze A. Jakwerth Ulrich M. Zissler Matthew D. Heath Markus Ollert Matthias F. Kramer Christiane Hilger Maria Beatrice Bilò Carsten B. Schmidt-Weber Simon Blank Allergen Content of Therapeutic Preparations for Allergen-Specific Immunotherapy of European Paper Wasp Venom Allergy Toxins allergen-specific immunotherapy American <i>Polistes</i> species European paper wasp European <i>Polistes</i> species Hymenoptera venom allergy <i>Polistes dominula</i> |
| title | Allergen Content of Therapeutic Preparations for Allergen-Specific Immunotherapy of European Paper Wasp Venom Allergy |
| title_full | Allergen Content of Therapeutic Preparations for Allergen-Specific Immunotherapy of European Paper Wasp Venom Allergy |
| title_fullStr | Allergen Content of Therapeutic Preparations for Allergen-Specific Immunotherapy of European Paper Wasp Venom Allergy |
| title_full_unstemmed | Allergen Content of Therapeutic Preparations for Allergen-Specific Immunotherapy of European Paper Wasp Venom Allergy |
| title_short | Allergen Content of Therapeutic Preparations for Allergen-Specific Immunotherapy of European Paper Wasp Venom Allergy |
| title_sort | allergen content of therapeutic preparations for allergen specific immunotherapy of european paper wasp venom allergy |
| topic | allergen-specific immunotherapy American <i>Polistes</i> species European paper wasp European <i>Polistes</i> species Hymenoptera venom allergy <i>Polistes dominula</i> |
| url | https://www.mdpi.com/2072-6651/14/4/284 |
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