| Summary: | The objective of this research was to investigate the feasibility of integrating oat (1 → 3, 1 → 4)-β-D-glucan (β-glucan) dried by two different techniques (freeze drying and spray drying) into a synbiotic formulation with <i>Akkermansia muciniphila</i>. The study evaluated the impact of this synbiotic formulation on the growth of <i>A. muciniphila</i> and its effect on the fermentation process. The extracted oat β-glucans underwent freeze-drying (FD) and spray-drying (SD) processes before being introduced as supplementary carbon sources (1%) to brain heart infusion (BHI) medium containing <i>A. muciniphila</i> MSCL 1582. The BHI medium containing inulin, D-glucose, and BHI without added substrates served as the control. Bacterial growth and short-chain fatty acid (SCFA) production were measured before and after 72 h of fermentation. A light microscope and KOVA slides were used for the <i>A. muciniphila</i> count, and SCFA levels were measured via gas chromatography. Our findings revealed that oat β-glucans could effectively function as prebiotic substrates in complementary synbiotic composition with <i>A. muciniphila</i>, without inhibiting growth and causing metabolic impairment. Both FD and SD techniques demonstrated equivalent and favorable impacts on the fermentative capacity of <i>A. muciniphila</i>, rendering them suitable choices for the drying of β-glucans. Incorporating oat β-glucan into synbiotic formulations offers potential benefits, contributing to <i>A. muciniphila</i> growth and the fermentation process.
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