The Feasibility of Integrating Spray-Dried and Freeze-Dried Oat β-Glucans into a Synbiotic Formulation with <i>Akkermansia muciniphila</i>
The objective of this research was to investigate the feasibility of integrating oat (1 → 3, 1 → 4)-β-D-glucan (β-glucan) dried by two different techniques (freeze drying and spray drying) into a synbiotic formulation with <i>Akkermansia muciniphila</i>. The study evaluated the impact of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/10/895 |
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| author | Vanda Sargautiene Darius Sargautis Anton Podjava Ida Jakobsone Vizma Nikolajeva |
| author_facet | Vanda Sargautiene Darius Sargautis Anton Podjava Ida Jakobsone Vizma Nikolajeva |
| author_sort | Vanda Sargautiene |
| collection | DOAJ |
| description | The objective of this research was to investigate the feasibility of integrating oat (1 → 3, 1 → 4)-β-D-glucan (β-glucan) dried by two different techniques (freeze drying and spray drying) into a synbiotic formulation with <i>Akkermansia muciniphila</i>. The study evaluated the impact of this synbiotic formulation on the growth of <i>A. muciniphila</i> and its effect on the fermentation process. The extracted oat β-glucans underwent freeze-drying (FD) and spray-drying (SD) processes before being introduced as supplementary carbon sources (1%) to brain heart infusion (BHI) medium containing <i>A. muciniphila</i> MSCL 1582. The BHI medium containing inulin, D-glucose, and BHI without added substrates served as the control. Bacterial growth and short-chain fatty acid (SCFA) production were measured before and after 72 h of fermentation. A light microscope and KOVA slides were used for the <i>A. muciniphila</i> count, and SCFA levels were measured via gas chromatography. Our findings revealed that oat β-glucans could effectively function as prebiotic substrates in complementary synbiotic composition with <i>A. muciniphila</i>, without inhibiting growth and causing metabolic impairment. Both FD and SD techniques demonstrated equivalent and favorable impacts on the fermentative capacity of <i>A. muciniphila</i>, rendering them suitable choices for the drying of β-glucans. Incorporating oat β-glucan into synbiotic formulations offers potential benefits, contributing to <i>A. muciniphila</i> growth and the fermentation process. |
| first_indexed | 2024-03-10T21:15:52Z |
| format | Article |
| id | doaj.art-9dd783af407f45acbd87263abb5cb7b5 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T21:15:52Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-9dd783af407f45acbd87263abb5cb7b52023-11-19T16:26:18ZengMDPI AGFermentation2311-56372023-10-0191089510.3390/fermentation9100895The Feasibility of Integrating Spray-Dried and Freeze-Dried Oat β-Glucans into a Synbiotic Formulation with <i>Akkermansia muciniphila</i>Vanda Sargautiene0Darius Sargautis1Anton Podjava2Ida Jakobsone3Vizma Nikolajeva4Faculty of Medicine, University of Latvia, 3 Jelgavas Str., LV-1004 Riga, LatviaFaculty of Food Technologies, Latvia University of Life Sciences and Technologies, 22 Riga Str., LV-3004 Jelgava, LatviaFaculty of Chemistry, University of Latvia, 1 Jelgavas Str., LV-1004 Riga, LatviaFaculty of Chemistry, University of Latvia, 1 Jelgavas Str., LV-1004 Riga, LatviaFaculty of Biology, University of Latvia, 1 Jelgavas Str., LV-1004 Riga, LatviaThe objective of this research was to investigate the feasibility of integrating oat (1 → 3, 1 → 4)-β-D-glucan (β-glucan) dried by two different techniques (freeze drying and spray drying) into a synbiotic formulation with <i>Akkermansia muciniphila</i>. The study evaluated the impact of this synbiotic formulation on the growth of <i>A. muciniphila</i> and its effect on the fermentation process. The extracted oat β-glucans underwent freeze-drying (FD) and spray-drying (SD) processes before being introduced as supplementary carbon sources (1%) to brain heart infusion (BHI) medium containing <i>A. muciniphila</i> MSCL 1582. The BHI medium containing inulin, D-glucose, and BHI without added substrates served as the control. Bacterial growth and short-chain fatty acid (SCFA) production were measured before and after 72 h of fermentation. A light microscope and KOVA slides were used for the <i>A. muciniphila</i> count, and SCFA levels were measured via gas chromatography. Our findings revealed that oat β-glucans could effectively function as prebiotic substrates in complementary synbiotic composition with <i>A. muciniphila</i>, without inhibiting growth and causing metabolic impairment. Both FD and SD techniques demonstrated equivalent and favorable impacts on the fermentative capacity of <i>A. muciniphila</i>, rendering them suitable choices for the drying of β-glucans. Incorporating oat β-glucan into synbiotic formulations offers potential benefits, contributing to <i>A. muciniphila</i> growth and the fermentation process.https://www.mdpi.com/2311-5637/9/10/895spray dryingfreeze dryingoat β-glucan<i>Akkermansia muciniphila</i>synbiotic formulationfermentation |
| spellingShingle | Vanda Sargautiene Darius Sargautis Anton Podjava Ida Jakobsone Vizma Nikolajeva The Feasibility of Integrating Spray-Dried and Freeze-Dried Oat β-Glucans into a Synbiotic Formulation with <i>Akkermansia muciniphila</i> Fermentation spray drying freeze drying oat β-glucan <i>Akkermansia muciniphila</i> synbiotic formulation fermentation |
| title | The Feasibility of Integrating Spray-Dried and Freeze-Dried Oat β-Glucans into a Synbiotic Formulation with <i>Akkermansia muciniphila</i> |
| title_full | The Feasibility of Integrating Spray-Dried and Freeze-Dried Oat β-Glucans into a Synbiotic Formulation with <i>Akkermansia muciniphila</i> |
| title_fullStr | The Feasibility of Integrating Spray-Dried and Freeze-Dried Oat β-Glucans into a Synbiotic Formulation with <i>Akkermansia muciniphila</i> |
| title_full_unstemmed | The Feasibility of Integrating Spray-Dried and Freeze-Dried Oat β-Glucans into a Synbiotic Formulation with <i>Akkermansia muciniphila</i> |
| title_short | The Feasibility of Integrating Spray-Dried and Freeze-Dried Oat β-Glucans into a Synbiotic Formulation with <i>Akkermansia muciniphila</i> |
| title_sort | feasibility of integrating spray dried and freeze dried oat β glucans into a synbiotic formulation with i akkermansia muciniphila i |
| topic | spray drying freeze drying oat β-glucan <i>Akkermansia muciniphila</i> synbiotic formulation fermentation |
| url | https://www.mdpi.com/2311-5637/9/10/895 |
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