Occurrence of Squash yellow mild mottle virus and Pepper golden mosaic virus in Potential New Hosts in Costa Rica
Leaf samples of Solanum lycopersicum, Capsicum annuum, Cucurbita moschata, Cucurbita pepo, Sechium edule and Erythrina spp. were collected. All samples were positive for begomoviruses using polymerase chain reaction and degenerate primers. A sequence of ∼1,100 bp was obtained from the genomic compon...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hanrimwon Publishing Company
2013-09-01
|
| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174809/ |
| _version_ | 1818558722612396032 |
|---|---|
| author | Ruth M. Castro Lisela Moreira María R. Rojas Robert L. Gilbertson Eduardo Hernández Floribeth Mora Pilar Ramírez |
| author_facet | Ruth M. Castro Lisela Moreira María R. Rojas Robert L. Gilbertson Eduardo Hernández Floribeth Mora Pilar Ramírez |
| author_sort | Ruth M. Castro |
| collection | DOAJ |
| description | Leaf samples of Solanum lycopersicum, Capsicum annuum, Cucurbita moschata, Cucurbita pepo, Sechium edule and Erythrina spp. were collected. All samples were positive for begomoviruses using polymerase chain reaction and degenerate primers. A sequence of ∼1,100 bp was obtained from the genomic component DNA-A of 14 samples. In addition, one sequence of ∼580 bp corresponding to the coat protein (AV1) was obtained from a chayote (S. edule) leaf sample. The presence of Squash yellow mild mottle virus (SYMMoV) and Pepper golden mosaic virus (PepGMV) were confirmed. The host range reported for SYMMoV includes species of the Cucurbitaceae, Caricaceae and Fabaceae families. This report extends the host range of SYMMoV to include the Solanaceae family, and extends the host range of PepGMV to include C. moschata, C. pepo and the Fabaceae Erythrina spp. This is the first report of a begomovirus (PepGMV) infecting chayote in the Western Hemisphere. |
| first_indexed | 2024-12-14T00:16:01Z |
| format | Article |
| id | doaj.art-9ddcd2266e18459b8e40ac3301abf5e1 |
| institution | Directory Open Access Journal |
| issn | 1598-2254 |
| language | English |
| last_indexed | 2024-12-14T00:16:01Z |
| publishDate | 2013-09-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-9ddcd2266e18459b8e40ac3301abf5e12022-12-21T23:25:33ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542013-09-0129328529310.5423/PPJ.OA.12.2012.0182PPJ.OA.12.2012.0182Occurrence of Squash yellow mild mottle virus and Pepper golden mosaic virus in Potential New Hosts in Costa RicaRuth M. Castro0Lisela Moreira1María R. Rojas2Robert L. Gilbertson3Eduardo Hernández4Floribeth Mora5Pilar Ramírez6Escuela de Ciencias Agrarias, Universidad Nacional, Heredia, Costa RicaCentro de Investigación en Biología Celular y Molecular, Universidad de Costa Rica, CP 11501-2060, San Jose, Costa RicaPlant Pathology Department, University of California-Davis, California, USAPlant Pathology Department, University of California-Davis, California, USACentro de Investigación en Biología Celular y Molecular, Universidad de Costa Rica, CP 11501-2060, San Jose, Costa RicaCentro de Investigación en Biología Celular y Molecular, Universidad de Costa Rica, CP 11501-2060, San Jose, Costa RicaCentro de Investigación en Biología Celular y Molecular, Universidad de Costa Rica, CP 11501-2060, San Jose, Costa RicaLeaf samples of Solanum lycopersicum, Capsicum annuum, Cucurbita moschata, Cucurbita pepo, Sechium edule and Erythrina spp. were collected. All samples were positive for begomoviruses using polymerase chain reaction and degenerate primers. A sequence of ∼1,100 bp was obtained from the genomic component DNA-A of 14 samples. In addition, one sequence of ∼580 bp corresponding to the coat protein (AV1) was obtained from a chayote (S. edule) leaf sample. The presence of Squash yellow mild mottle virus (SYMMoV) and Pepper golden mosaic virus (PepGMV) were confirmed. The host range reported for SYMMoV includes species of the Cucurbitaceae, Caricaceae and Fabaceae families. This report extends the host range of SYMMoV to include the Solanaceae family, and extends the host range of PepGMV to include C. moschata, C. pepo and the Fabaceae Erythrina spp. This is the first report of a begomovirus (PepGMV) infecting chayote in the Western Hemisphere.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174809/begomovirusvegetableswhiteflies |
| spellingShingle | Ruth M. Castro Lisela Moreira María R. Rojas Robert L. Gilbertson Eduardo Hernández Floribeth Mora Pilar Ramírez Occurrence of Squash yellow mild mottle virus and Pepper golden mosaic virus in Potential New Hosts in Costa Rica The Plant Pathology Journal begomovirus vegetables whiteflies |
| title | Occurrence of Squash yellow mild mottle virus and Pepper golden mosaic virus in Potential New Hosts in Costa Rica |
| title_full | Occurrence of Squash yellow mild mottle virus and Pepper golden mosaic virus in Potential New Hosts in Costa Rica |
| title_fullStr | Occurrence of Squash yellow mild mottle virus and Pepper golden mosaic virus in Potential New Hosts in Costa Rica |
| title_full_unstemmed | Occurrence of Squash yellow mild mottle virus and Pepper golden mosaic virus in Potential New Hosts in Costa Rica |
| title_short | Occurrence of Squash yellow mild mottle virus and Pepper golden mosaic virus in Potential New Hosts in Costa Rica |
| title_sort | occurrence of squash yellow mild mottle virus and pepper golden mosaic virus in potential new hosts in costa rica |
| topic | begomovirus vegetables whiteflies |
| url | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174809/ |
| work_keys_str_mv | AT ruthmcastro occurrenceofsquashyellowmildmottlevirusandpeppergoldenmosaicvirusinpotentialnewhostsincostarica AT liselamoreira occurrenceofsquashyellowmildmottlevirusandpeppergoldenmosaicvirusinpotentialnewhostsincostarica AT mariarrojas occurrenceofsquashyellowmildmottlevirusandpeppergoldenmosaicvirusinpotentialnewhostsincostarica AT robertlgilbertson occurrenceofsquashyellowmildmottlevirusandpeppergoldenmosaicvirusinpotentialnewhostsincostarica AT eduardohernandez occurrenceofsquashyellowmildmottlevirusandpeppergoldenmosaicvirusinpotentialnewhostsincostarica AT floribethmora occurrenceofsquashyellowmildmottlevirusandpeppergoldenmosaicvirusinpotentialnewhostsincostarica AT pilarramirez occurrenceofsquashyellowmildmottlevirusandpeppergoldenmosaicvirusinpotentialnewhostsincostarica |