Genome-wide identification, classification, and expression analysis of the HSF gene family in pineapple (Ananas comosus)
Transcription factors (TFs), such as heat shock transcription factors (HSFs), usually play critical regulatory functions in plant development, growth, and response to environmental cues. However, no HSFs have been characterized in pineapple thus far. Here, we identified 22 AcHSF genes from the pinea...
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PeerJ Inc.
2021-04-01
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| author | Lulu Wang Yanhui Liu Mengnan Chai Huihuang Chen Mohammad Aslam Xiaoping Niu Yuan Qin Hanyang Cai |
| author_facet | Lulu Wang Yanhui Liu Mengnan Chai Huihuang Chen Mohammad Aslam Xiaoping Niu Yuan Qin Hanyang Cai |
| author_sort | Lulu Wang |
| collection | DOAJ |
| description | Transcription factors (TFs), such as heat shock transcription factors (HSFs), usually play critical regulatory functions in plant development, growth, and response to environmental cues. However, no HSFs have been characterized in pineapple thus far. Here, we identified 22 AcHSF genes from the pineapple genome. Gene structure, motifs, and phylogenetic analysis showed that AcHSF families were distinctly grouped into three subfamilies (12 in Group A, seven in Group B, and four in Group C). The AcHSF promoters contained various cis-elements associated with stress, hormones, and plant development processes, for instance, STRE, WRKY, and ABRE binding sites. The majority of HSFs were expressed in diverse pineapple tissues and developmental stages. The expression of AcHSF-B4b/AcHSF-B4c and AcHSF-A7b/AcHSF-A1c were enriched in the ovules and fruits, respectively. Six genes (AcHSF-A1a , AcHSF-A2, AcHSF-A9a, AcHSF-B1a, AcHSF-B2a, and AcHSF-C1a) were transcriptionally modified by cold, heat, and ABA. Our results provide an overview and lay the foundation for future functional characterization of the pineapple HSF gene family. |
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| language | English |
| last_indexed | 2024-03-09T07:23:04Z |
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| spelling | doaj.art-9dde5faf1be1486d973dc108d3f28fee2023-12-03T07:14:16ZengPeerJ Inc.PeerJ2167-83592021-04-019e1132910.7717/peerj.11329Genome-wide identification, classification, and expression analysis of the HSF gene family in pineapple (Ananas comosus)Lulu Wang0Yanhui Liu1Mengnan Chai2Huihuang Chen3Mohammad Aslam4Xiaoping Niu5Yuan Qin6Hanyang Cai7State Key Lab of Ecological Pest Control for Fujian and Taiwan Crops; Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education; Fujian Provincial Key Lab of Haixia Applied Plant Systems Biology, College of Life Sciences, Fuji, Fuzhou, Fujian, ChinaState Key Lab of Ecological Pest Control for Fujian and Taiwan Crops; Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education; Fujian Provincial Key Lab of Haixia Applied Plant Systems Biology, College of Life Sciences, Fuji, Fuzhou, Fujian, ChinaState Key Lab of Ecological Pest Control for Fujian and Taiwan Crops; Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education; Fujian Provincial Key Lab of Haixia Applied Plant Systems Biology, College of Life Sciences, Fuji, Fuzhou, Fujian, ChinaState Key Lab of Ecological Pest Control for Fujian and Taiwan Crops; Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education; Fujian Provincial Key Lab of Haixia Applied Plant Systems Biology, College of Life Sciences, Fuji, Fuzhou, Fujian, ChinaState Key Lab of Ecological Pest Control for Fujian and Taiwan Crops; Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education; Fujian Provincial Key Lab of Haixia Applied Plant Systems Biology, College of Life Sciences, Fuji, Fuzhou, Fujian, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Lab of Sugarcane Biology, College of Agriculture, Guangxi University, Nanning, Guangxi, ChinaState Key Lab of Ecological Pest Control for Fujian and Taiwan Crops; Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education; Fujian Provincial Key Lab of Haixia Applied Plant Systems Biology, College of Life Sciences, Fuji, Fuzhou, Fujian, ChinaState Key Lab of Ecological Pest Control for Fujian and Taiwan Crops; Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education; Fujian Provincial Key Lab of Haixia Applied Plant Systems Biology, College of Life Sciences, Fuji, Fuzhou, Fujian, ChinaTranscription factors (TFs), such as heat shock transcription factors (HSFs), usually play critical regulatory functions in plant development, growth, and response to environmental cues. However, no HSFs have been characterized in pineapple thus far. Here, we identified 22 AcHSF genes from the pineapple genome. Gene structure, motifs, and phylogenetic analysis showed that AcHSF families were distinctly grouped into three subfamilies (12 in Group A, seven in Group B, and four in Group C). The AcHSF promoters contained various cis-elements associated with stress, hormones, and plant development processes, for instance, STRE, WRKY, and ABRE binding sites. The majority of HSFs were expressed in diverse pineapple tissues and developmental stages. The expression of AcHSF-B4b/AcHSF-B4c and AcHSF-A7b/AcHSF-A1c were enriched in the ovules and fruits, respectively. Six genes (AcHSF-A1a , AcHSF-A2, AcHSF-A9a, AcHSF-B1a, AcHSF-B2a, and AcHSF-C1a) were transcriptionally modified by cold, heat, and ABA. Our results provide an overview and lay the foundation for future functional characterization of the pineapple HSF gene family.https://peerj.com/articles/11329.pdfAcHSF Phylogenetic analysis Pineapple Cold Heat ABA |
| spellingShingle | Lulu Wang Yanhui Liu Mengnan Chai Huihuang Chen Mohammad Aslam Xiaoping Niu Yuan Qin Hanyang Cai Genome-wide identification, classification, and expression analysis of the HSF gene family in pineapple (Ananas comosus) PeerJ AcHSF Phylogenetic analysis Pineapple Cold Heat ABA |
| title | Genome-wide identification, classification, and expression analysis of the HSF gene family in pineapple (Ananas comosus) |
| title_full | Genome-wide identification, classification, and expression analysis of the HSF gene family in pineapple (Ananas comosus) |
| title_fullStr | Genome-wide identification, classification, and expression analysis of the HSF gene family in pineapple (Ananas comosus) |
| title_full_unstemmed | Genome-wide identification, classification, and expression analysis of the HSF gene family in pineapple (Ananas comosus) |
| title_short | Genome-wide identification, classification, and expression analysis of the HSF gene family in pineapple (Ananas comosus) |
| title_sort | genome wide identification classification and expression analysis of the hsf gene family in pineapple ananas comosus |
| topic | AcHSF Phylogenetic analysis Pineapple Cold Heat ABA |
| url | https://peerj.com/articles/11329.pdf |
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