Global Changes in Gene Expression in Sinorhizobium meliloti 1021 under Microoxic and Symbiotic Conditions
Sinorhizobium meliloti is an α-proteobacterium that alternates between a free-living phase in bulk soil or in the rhizosphere of plants and a symbiotic phase within the host plant cells, where the bacteria ultimately differentiate into nitrogen-fixing organelle-like cells, called bacteroids. As a st...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2004-03-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2004.17.3.292 |
| _version_ | 1818914954111090688 |
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| author | Anke Becker Hélène Bergès Elizaveta Krol Claude Bruand Silvia Rüberg Delphine Capela Emmanuelle Lauber Eliane Meilhoc Frédéric Ampe Frans J. de Bruijn Joëlle Fourment Anne Francez-Charlot Daniel Kahn Helge Küster Carine Liebe Alfred Pühler Stefan Weidner Jacques Batut |
| author_facet | Anke Becker Hélène Bergès Elizaveta Krol Claude Bruand Silvia Rüberg Delphine Capela Emmanuelle Lauber Eliane Meilhoc Frédéric Ampe Frans J. de Bruijn Joëlle Fourment Anne Francez-Charlot Daniel Kahn Helge Küster Carine Liebe Alfred Pühler Stefan Weidner Jacques Batut |
| author_sort | Anke Becker |
| collection | DOAJ |
| description | Sinorhizobium meliloti is an α-proteobacterium that alternates between a free-living phase in bulk soil or in the rhizosphere of plants and a symbiotic phase within the host plant cells, where the bacteria ultimately differentiate into nitrogen-fixing organelle-like cells, called bacteroids. As a step toward understanding the physiology of S. meliloti in its free-living and symbiotic forms and the transition between the two, gene expression profiles were determined under two sets of biological conditions: growth under oxic versus microoxic conditions, and in free-living versus symbiotic state. Data acquisition was based on both macro- and microarrays. Transcriptome profiles highlighted a profound modification of gene expression during bacteroid differentiation, with 16% of genes being altered. The data are consistent with an overall slow down of bacteroid metabolism during adaptation to symbiotic life and acquisition of nitrogen fixation capability. A large number of genes of unknown function, including potential regulators, that may play a role in symbiosis were identified. Transcriptome profiling in response to oxygen limitation indicated that up to 5% of the genes were oxygen regulated. However, the microoxic and bacteroid transcriptomes only partially overlap, implying that oxygen contributes to a limited extent to the control of symbiotic gene expression. |
| first_indexed | 2024-12-19T23:54:35Z |
| format | Article |
| id | doaj.art-9ddede56eb2141d78baee7c12877651b |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-19T23:54:35Z |
| publishDate | 2004-03-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-9ddede56eb2141d78baee7c12877651b2022-12-21T20:01:03ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062004-03-0117329230310.1094/MPMI.2004.17.3.292Global Changes in Gene Expression in Sinorhizobium meliloti 1021 under Microoxic and Symbiotic ConditionsAnke BeckerHélène BergèsElizaveta KrolClaude BruandSilvia RübergDelphine CapelaEmmanuelle LauberEliane MeilhocFrédéric AmpeFrans J. de BruijnJoëlle FourmentAnne Francez-CharlotDaniel KahnHelge KüsterCarine LiebeAlfred PühlerStefan WeidnerJacques BatutSinorhizobium meliloti is an α-proteobacterium that alternates between a free-living phase in bulk soil or in the rhizosphere of plants and a symbiotic phase within the host plant cells, where the bacteria ultimately differentiate into nitrogen-fixing organelle-like cells, called bacteroids. As a step toward understanding the physiology of S. meliloti in its free-living and symbiotic forms and the transition between the two, gene expression profiles were determined under two sets of biological conditions: growth under oxic versus microoxic conditions, and in free-living versus symbiotic state. Data acquisition was based on both macro- and microarrays. Transcriptome profiles highlighted a profound modification of gene expression during bacteroid differentiation, with 16% of genes being altered. The data are consistent with an overall slow down of bacteroid metabolism during adaptation to symbiotic life and acquisition of nitrogen fixation capability. A large number of genes of unknown function, including potential regulators, that may play a role in symbiosis were identified. Transcriptome profiling in response to oxygen limitation indicated that up to 5% of the genes were oxygen regulated. However, the microoxic and bacteroid transcriptomes only partially overlap, implying that oxygen contributes to a limited extent to the control of symbiotic gene expression.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2004.17.3.292macroarrayroot nodule |
| spellingShingle | Anke Becker Hélène Bergès Elizaveta Krol Claude Bruand Silvia Rüberg Delphine Capela Emmanuelle Lauber Eliane Meilhoc Frédéric Ampe Frans J. de Bruijn Joëlle Fourment Anne Francez-Charlot Daniel Kahn Helge Küster Carine Liebe Alfred Pühler Stefan Weidner Jacques Batut Global Changes in Gene Expression in Sinorhizobium meliloti 1021 under Microoxic and Symbiotic Conditions Molecular Plant-Microbe Interactions macroarray root nodule |
| title | Global Changes in Gene Expression in Sinorhizobium meliloti 1021 under Microoxic and Symbiotic Conditions |
| title_full | Global Changes in Gene Expression in Sinorhizobium meliloti 1021 under Microoxic and Symbiotic Conditions |
| title_fullStr | Global Changes in Gene Expression in Sinorhizobium meliloti 1021 under Microoxic and Symbiotic Conditions |
| title_full_unstemmed | Global Changes in Gene Expression in Sinorhizobium meliloti 1021 under Microoxic and Symbiotic Conditions |
| title_short | Global Changes in Gene Expression in Sinorhizobium meliloti 1021 under Microoxic and Symbiotic Conditions |
| title_sort | global changes in gene expression in sinorhizobium meliloti 1021 under microoxic and symbiotic conditions |
| topic | macroarray root nodule |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2004.17.3.292 |
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