Nitric Oxide regulates mouth development in amphioxus
Abstract The development of the mouth in animals has fascinated researchers for decades, and a recent study proposed the modern view of recurrent evolution of protostomy and deuterostomy. Here we expanded our knowledge about conserved traits of mouth formation in chordates, testing the hypothesis th...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-08157-w |
| _version_ | 1818754273130840064 |
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| author | Giovanni Annona Filomena Caccavale Juan Pascual-Anaya Shigeru Kuratani Pasquale De Luca Anna Palumbo Salvatore D’Aniello |
| author_facet | Giovanni Annona Filomena Caccavale Juan Pascual-Anaya Shigeru Kuratani Pasquale De Luca Anna Palumbo Salvatore D’Aniello |
| author_sort | Giovanni Annona |
| collection | DOAJ |
| description | Abstract The development of the mouth in animals has fascinated researchers for decades, and a recent study proposed the modern view of recurrent evolution of protostomy and deuterostomy. Here we expanded our knowledge about conserved traits of mouth formation in chordates, testing the hypothesis that nitric oxide (NO) is a potential regulator of this process. In the present work we show for the first time that NO is an essential cell signaling molecule for cephalochordate mouth formation, as previously shown for vertebrates, indicating its conserved ancestral role in chordates. The experimental decrease of NO during early amphioxus Branchiostoma lanceolatum development impaired the formation of the mouth and gill slits, demonstrating that it is a prerequisite in pharyngeal morphogenesis. Our results represent the first step in the understanding of NO physiology in non-vertebrate chordates, opening new evolutionary perspectives into the ancestral importance of NO homeostasis and acquisition of novel biological roles during evolution. |
| first_indexed | 2024-12-18T05:20:38Z |
| format | Article |
| id | doaj.art-9de11b66dcdc4b5ebca123017304a261 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T05:20:38Z |
| publishDate | 2017-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9de11b66dcdc4b5ebca123017304a2612022-12-21T21:19:40ZengNature PortfolioScientific Reports2045-23222017-08-017111010.1038/s41598-017-08157-wNitric Oxide regulates mouth development in amphioxusGiovanni Annona0Filomena Caccavale1Juan Pascual-Anaya2Shigeru Kuratani3Pasquale De Luca4Anna Palumbo5Salvatore D’Aniello6Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn di NapoliBiology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn di NapoliEvolutionary Morphology Laboratory, RIKENEvolutionary Morphology Laboratory, RIKENRIMAR, Stazione Zoologica Anton Dohrn di NapoliBiology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn di NapoliBiology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn di NapoliAbstract The development of the mouth in animals has fascinated researchers for decades, and a recent study proposed the modern view of recurrent evolution of protostomy and deuterostomy. Here we expanded our knowledge about conserved traits of mouth formation in chordates, testing the hypothesis that nitric oxide (NO) is a potential regulator of this process. In the present work we show for the first time that NO is an essential cell signaling molecule for cephalochordate mouth formation, as previously shown for vertebrates, indicating its conserved ancestral role in chordates. The experimental decrease of NO during early amphioxus Branchiostoma lanceolatum development impaired the formation of the mouth and gill slits, demonstrating that it is a prerequisite in pharyngeal morphogenesis. Our results represent the first step in the understanding of NO physiology in non-vertebrate chordates, opening new evolutionary perspectives into the ancestral importance of NO homeostasis and acquisition of novel biological roles during evolution.https://doi.org/10.1038/s41598-017-08157-w |
| spellingShingle | Giovanni Annona Filomena Caccavale Juan Pascual-Anaya Shigeru Kuratani Pasquale De Luca Anna Palumbo Salvatore D’Aniello Nitric Oxide regulates mouth development in amphioxus Scientific Reports |
| title | Nitric Oxide regulates mouth development in amphioxus |
| title_full | Nitric Oxide regulates mouth development in amphioxus |
| title_fullStr | Nitric Oxide regulates mouth development in amphioxus |
| title_full_unstemmed | Nitric Oxide regulates mouth development in amphioxus |
| title_short | Nitric Oxide regulates mouth development in amphioxus |
| title_sort | nitric oxide regulates mouth development in amphioxus |
| url | https://doi.org/10.1038/s41598-017-08157-w |
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