Ectopic Expression of <i>OLEOSIN 1</i> and Inactivation of <i>GBSS1</i> Have a Synergistic Effect on Oil Accumulation in Plant Leaves
During the transformation of wild-type (WT) <i>Arabidopsis thaliana</i>, a T-DNA containing <i>OLEOSIN-GFP</i> (<i>OLE1-GFP</i>) was inserted by happenstance within the <i>GBSS1</i> gene, resulting in significant reduction in amylose and increase in le...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/3/513 |
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| author | Zhiyang Zhai Hui Liu John Shanklin |
| author_facet | Zhiyang Zhai Hui Liu John Shanklin |
| author_sort | Zhiyang Zhai |
| collection | DOAJ |
| description | During the transformation of wild-type (WT) <i>Arabidopsis thaliana</i>, a T-DNA containing <i>OLEOSIN-GFP</i> (<i>OLE1-GFP</i>) was inserted by happenstance within the <i>GBSS1</i> gene, resulting in significant reduction in amylose and increase in leaf oil content in the transgenic line (OG). The synergistic effect on oil accumulation of combining <i>gbss1</i> with the expression of <i>OLE1-GFP</i> was confirmed by transforming an independent <i>gbss1</i> mutant (GABI_914G01) with <i>OLE1-GFP.</i> The resulting <i>OLE1-GFP</i>/<i>gbss1</i> transgenic lines showed higher leaf oil content than the individual <i>OLE1-GFP/</i>WT or single <i>gbss1</i> mutant lines. Further stacking of the lipogenic factors WRINKLED1, Diacylglycerol O-Acyltransferase (DGAT1), and Cys-OLEOSIN1 (an engineered sesame OLEOSIN1) in <i>OG</i> significantly elevated its oil content in mature leaves to 2.3% of dry weight, which is 15 times higher than that in WT Arabidopsis. Inducible expression of the same lipogenic factors was shown to be an effective strategy for triacylglycerol (TAG) accumulation without incurring growth, development, and yield penalties. |
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| format | Article |
| id | doaj.art-9de35aa23c2944248ea9307f94546d0c |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T13:24:22Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-9de35aa23c2944248ea9307f94546d0c2023-11-21T09:48:52ZengMDPI AGPlants2223-77472021-03-0110351310.3390/plants10030513Ectopic Expression of <i>OLEOSIN 1</i> and Inactivation of <i>GBSS1</i> Have a Synergistic Effect on Oil Accumulation in Plant LeavesZhiyang Zhai0Hui Liu1John Shanklin2Biology Department, Brookhaven National Laboratory, BNL 463, 50 Bell Ave., Upton, NY 11953, USABiology Department, Brookhaven National Laboratory, BNL 463, 50 Bell Ave., Upton, NY 11953, USABiology Department, Brookhaven National Laboratory, BNL 463, 50 Bell Ave., Upton, NY 11953, USADuring the transformation of wild-type (WT) <i>Arabidopsis thaliana</i>, a T-DNA containing <i>OLEOSIN-GFP</i> (<i>OLE1-GFP</i>) was inserted by happenstance within the <i>GBSS1</i> gene, resulting in significant reduction in amylose and increase in leaf oil content in the transgenic line (OG). The synergistic effect on oil accumulation of combining <i>gbss1</i> with the expression of <i>OLE1-GFP</i> was confirmed by transforming an independent <i>gbss1</i> mutant (GABI_914G01) with <i>OLE1-GFP.</i> The resulting <i>OLE1-GFP</i>/<i>gbss1</i> transgenic lines showed higher leaf oil content than the individual <i>OLE1-GFP/</i>WT or single <i>gbss1</i> mutant lines. Further stacking of the lipogenic factors WRINKLED1, Diacylglycerol O-Acyltransferase (DGAT1), and Cys-OLEOSIN1 (an engineered sesame OLEOSIN1) in <i>OG</i> significantly elevated its oil content in mature leaves to 2.3% of dry weight, which is 15 times higher than that in WT Arabidopsis. Inducible expression of the same lipogenic factors was shown to be an effective strategy for triacylglycerol (TAG) accumulation without incurring growth, development, and yield penalties.https://www.mdpi.com/2223-7747/10/3/513triacylglycerolfatty acidsoleosingranule bound starch synthasemetabolic engineering |
| spellingShingle | Zhiyang Zhai Hui Liu John Shanklin Ectopic Expression of <i>OLEOSIN 1</i> and Inactivation of <i>GBSS1</i> Have a Synergistic Effect on Oil Accumulation in Plant Leaves Plants triacylglycerol fatty acids oleosin granule bound starch synthase metabolic engineering |
| title | Ectopic Expression of <i>OLEOSIN 1</i> and Inactivation of <i>GBSS1</i> Have a Synergistic Effect on Oil Accumulation in Plant Leaves |
| title_full | Ectopic Expression of <i>OLEOSIN 1</i> and Inactivation of <i>GBSS1</i> Have a Synergistic Effect on Oil Accumulation in Plant Leaves |
| title_fullStr | Ectopic Expression of <i>OLEOSIN 1</i> and Inactivation of <i>GBSS1</i> Have a Synergistic Effect on Oil Accumulation in Plant Leaves |
| title_full_unstemmed | Ectopic Expression of <i>OLEOSIN 1</i> and Inactivation of <i>GBSS1</i> Have a Synergistic Effect on Oil Accumulation in Plant Leaves |
| title_short | Ectopic Expression of <i>OLEOSIN 1</i> and Inactivation of <i>GBSS1</i> Have a Synergistic Effect on Oil Accumulation in Plant Leaves |
| title_sort | ectopic expression of i oleosin 1 i and inactivation of i gbss1 i have a synergistic effect on oil accumulation in plant leaves |
| topic | triacylglycerol fatty acids oleosin granule bound starch synthase metabolic engineering |
| url | https://www.mdpi.com/2223-7747/10/3/513 |
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