CRISPR-Cas12a exploits R-loop asymmetry to form double-strand breaks
Type V CRISPR-Cas interference proteins use a single RuvC active site to make RNA-guided breaks in double-stranded DNA substrates, an activity essential for both bacterial immunity and genome editing. The best-studied of these enzymes, Cas12a, initiates DNA cutting by forming a 20-nucleotide R-loop...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
eLife Sciences Publications Ltd
2020-06-01
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Series: | eLife |
Subjects: | |
Online Access: | https://elifesciences.org/articles/55143 |