Molecular identification and characterization of Anaplasma capra and Anaplasma platys-like in Rhipicephalus microplus in Ankang, Northwest China
Abstract Background Four species within Anaplasma genus are emerging zoonotic pathogens, which are transmitted by ticks and generate veterinary and public health concerns. Here, we performed a molecular survey of Anaplasma in Ankang, Northwest China. Methods Hard ticks were collected and identified...
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | http://link.springer.com/article/10.1186/s12879-019-4075-3 |
| _version_ | 1818925463016308736 |
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| author | Wen-Ping Guo Bing Zhang Yi-Han Wang Gang Xu Xiaoquan Wang Xuebing Ni En-Min Zhou |
| author_facet | Wen-Ping Guo Bing Zhang Yi-Han Wang Gang Xu Xiaoquan Wang Xuebing Ni En-Min Zhou |
| author_sort | Wen-Ping Guo |
| collection | DOAJ |
| description | Abstract Background Four species within Anaplasma genus are emerging zoonotic pathogens, which are transmitted by ticks and generate veterinary and public health concerns. Here, we performed a molecular survey of Anaplasma in Ankang, Northwest China. Methods Hard ticks were collected and identified using morphological and molecular methods. Human-pathogenic Anaplasma species were tested using nested polymerase chain reaction. The nearly complete rrs, gltA, and groEL genes sequences from revealed Anaplasma species were amplified and sequenced to determine their molecular characteristics and their phylogeny. Results All ticks collected in Ankang belonged to the Rhipicephalus microplus. Novel unclassified Anaplasma strains genetically related to A. platys and A. capra were detected in these ticks. Co-infection of these two organisms was also found. The novel unclassified Anaplasma strains identified in this study formed a distinct phylogenetic lineage based on the groEL gene and two lineages based on the gltA gene within A. platys and related strains group. The revealed A. capra strains identified in this study were most closely related to those detected in humans and other vertebrate animals. Conclusion We revealed the presence of A. capra, a novel human pathogens in R. microplus ticks in previously unrecognized endemic regions. We also detected a novel unclassified Anaplasma species genetically related to A. platys. The epidemiology of anaplasmosis caused by these two Anaplasma species in humans should be assessed in future studies. |
| first_indexed | 2024-12-20T02:41:37Z |
| format | Article |
| id | doaj.art-9de9955631d84740b9278752d0dab65a |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-20T02:41:37Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-9de9955631d84740b9278752d0dab65a2022-12-21T19:56:17ZengBMCBMC Infectious Diseases1471-23342019-05-011911910.1186/s12879-019-4075-3Molecular identification and characterization of Anaplasma capra and Anaplasma platys-like in Rhipicephalus microplus in Ankang, Northwest ChinaWen-Ping Guo0Bing Zhang1Yi-Han Wang2Gang Xu3Xiaoquan Wang4Xuebing Ni5En-Min Zhou6Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F UniversityDepartment of Human parasitology, Preclinical Medicine College, Xinjiang Medical UniversityDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F UniversityDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F UniversityXuwang Town Comprehensive Agricultural Service StationState Key laboratory of Emerging infectious disease, School of Public Health, The University of Hong KongDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F UniversityAbstract Background Four species within Anaplasma genus are emerging zoonotic pathogens, which are transmitted by ticks and generate veterinary and public health concerns. Here, we performed a molecular survey of Anaplasma in Ankang, Northwest China. Methods Hard ticks were collected and identified using morphological and molecular methods. Human-pathogenic Anaplasma species were tested using nested polymerase chain reaction. The nearly complete rrs, gltA, and groEL genes sequences from revealed Anaplasma species were amplified and sequenced to determine their molecular characteristics and their phylogeny. Results All ticks collected in Ankang belonged to the Rhipicephalus microplus. Novel unclassified Anaplasma strains genetically related to A. platys and A. capra were detected in these ticks. Co-infection of these two organisms was also found. The novel unclassified Anaplasma strains identified in this study formed a distinct phylogenetic lineage based on the groEL gene and two lineages based on the gltA gene within A. platys and related strains group. The revealed A. capra strains identified in this study were most closely related to those detected in humans and other vertebrate animals. Conclusion We revealed the presence of A. capra, a novel human pathogens in R. microplus ticks in previously unrecognized endemic regions. We also detected a novel unclassified Anaplasma species genetically related to A. platys. The epidemiology of anaplasmosis caused by these two Anaplasma species in humans should be assessed in future studies.http://link.springer.com/article/10.1186/s12879-019-4075-3Anaplasma capraAnaplasma platys-like strainsChinaRhipicephalus microplus ticksRrsgltA |
| spellingShingle | Wen-Ping Guo Bing Zhang Yi-Han Wang Gang Xu Xiaoquan Wang Xuebing Ni En-Min Zhou Molecular identification and characterization of Anaplasma capra and Anaplasma platys-like in Rhipicephalus microplus in Ankang, Northwest China BMC Infectious Diseases Anaplasma capra Anaplasma platys-like strains China Rhipicephalus microplus ticks Rrs gltA |
| title | Molecular identification and characterization of Anaplasma capra and Anaplasma platys-like in Rhipicephalus microplus in Ankang, Northwest China |
| title_full | Molecular identification and characterization of Anaplasma capra and Anaplasma platys-like in Rhipicephalus microplus in Ankang, Northwest China |
| title_fullStr | Molecular identification and characterization of Anaplasma capra and Anaplasma platys-like in Rhipicephalus microplus in Ankang, Northwest China |
| title_full_unstemmed | Molecular identification and characterization of Anaplasma capra and Anaplasma platys-like in Rhipicephalus microplus in Ankang, Northwest China |
| title_short | Molecular identification and characterization of Anaplasma capra and Anaplasma platys-like in Rhipicephalus microplus in Ankang, Northwest China |
| title_sort | molecular identification and characterization of anaplasma capra and anaplasma platys like in rhipicephalus microplus in ankang northwest china |
| topic | Anaplasma capra Anaplasma platys-like strains China Rhipicephalus microplus ticks Rrs gltA |
| url | http://link.springer.com/article/10.1186/s12879-019-4075-3 |
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