Experimental exposure assessment for in vitro cell-based bioassays in 96- and 384-well plates
In vitro cell-based bioassays have great potential for applications in the human health risk assessment of chemicals. The quantification of freely dissolved concentrations (Cfree) in in vitro assays is essential to generate reliable data for in vitro-to-in vivo extrapolation. Existing methods for th...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Toxicology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/ftox.2023.1221625/full |
| _version_ | 1797772952505155584 |
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| author | Julia Huchthausen Maria König Beate I. Escher Beate I. Escher Luise Henneberger |
| author_facet | Julia Huchthausen Maria König Beate I. Escher Beate I. Escher Luise Henneberger |
| author_sort | Julia Huchthausen |
| collection | DOAJ |
| description | In vitro cell-based bioassays have great potential for applications in the human health risk assessment of chemicals. The quantification of freely dissolved concentrations (Cfree) in in vitro assays is essential to generate reliable data for in vitro-to-in vivo extrapolation. Existing methods for the quantification of Cfree are limited to low-throughput microtiter plates. The present study is a proof of principle for the applicability of a solid-phase microextraction (SPME) method for the determination of Cfree in the peroxisome proliferator-activated receptor gamma (PPARγ) bioassay run in 384-well plates with 80 µL medium per well. The effect concentrations obtained from 384-well plates were compared with those obtained from 96-well plates in a previous study. Nominal effect concentrations obtained using 96- and 384-well plates agreed with each other within a factor of three, and freely dissolved effect concentrations agreed within a factor of 6.5. The good degree of agreement in the results from both plate formats proves the general applicability of the SPME method for the determination of Cfree for bioassays in 384-well plates, making the present study a first step toward exposure assessment in high-throughput bioassays. |
| first_indexed | 2024-03-12T21:59:15Z |
| format | Article |
| id | doaj.art-9dea9382fa4d4325a2b836b840158cd6 |
| institution | Directory Open Access Journal |
| issn | 2673-3080 |
| language | English |
| last_indexed | 2024-03-12T21:59:15Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Toxicology |
| spelling | doaj.art-9dea9382fa4d4325a2b836b840158cd62023-07-25T10:19:15ZengFrontiers Media S.A.Frontiers in Toxicology2673-30802023-07-01510.3389/ftox.2023.12216251221625Experimental exposure assessment for in vitro cell-based bioassays in 96- and 384-well platesJulia Huchthausen0Maria König1Beate I. Escher2Beate I. Escher3Luise Henneberger4Department of Cell Toxicology, Helmholtz Centre for Environmental Research—UFZ, Leipzig, GermanyDepartment of Cell Toxicology, Helmholtz Centre for Environmental Research—UFZ, Leipzig, GermanyDepartment of Cell Toxicology, Helmholtz Centre for Environmental Research—UFZ, Leipzig, GermanyDepartment of Geosciences, Environmental Toxicology, Eberhard Karls University Tübingen, Tübingen, GermanyDepartment of Cell Toxicology, Helmholtz Centre for Environmental Research—UFZ, Leipzig, GermanyIn vitro cell-based bioassays have great potential for applications in the human health risk assessment of chemicals. The quantification of freely dissolved concentrations (Cfree) in in vitro assays is essential to generate reliable data for in vitro-to-in vivo extrapolation. Existing methods for the quantification of Cfree are limited to low-throughput microtiter plates. The present study is a proof of principle for the applicability of a solid-phase microextraction (SPME) method for the determination of Cfree in the peroxisome proliferator-activated receptor gamma (PPARγ) bioassay run in 384-well plates with 80 µL medium per well. The effect concentrations obtained from 384-well plates were compared with those obtained from 96-well plates in a previous study. Nominal effect concentrations obtained using 96- and 384-well plates agreed with each other within a factor of three, and freely dissolved effect concentrations agreed within a factor of 6.5. The good degree of agreement in the results from both plate formats proves the general applicability of the SPME method for the determination of Cfree for bioassays in 384-well plates, making the present study a first step toward exposure assessment in high-throughput bioassays.https://www.frontiersin.org/articles/10.3389/ftox.2023.1221625/fullsolid-phase microextractionbioassayin vitrohigh throughputPPARγ |
| spellingShingle | Julia Huchthausen Maria König Beate I. Escher Beate I. Escher Luise Henneberger Experimental exposure assessment for in vitro cell-based bioassays in 96- and 384-well plates Frontiers in Toxicology solid-phase microextraction bioassay in vitro high throughput PPARγ |
| title | Experimental exposure assessment for in vitro cell-based bioassays in 96- and 384-well plates |
| title_full | Experimental exposure assessment for in vitro cell-based bioassays in 96- and 384-well plates |
| title_fullStr | Experimental exposure assessment for in vitro cell-based bioassays in 96- and 384-well plates |
| title_full_unstemmed | Experimental exposure assessment for in vitro cell-based bioassays in 96- and 384-well plates |
| title_short | Experimental exposure assessment for in vitro cell-based bioassays in 96- and 384-well plates |
| title_sort | experimental exposure assessment for in vitro cell based bioassays in 96 and 384 well plates |
| topic | solid-phase microextraction bioassay in vitro high throughput PPARγ |
| url | https://www.frontiersin.org/articles/10.3389/ftox.2023.1221625/full |
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