In vitro and in vivo evaluation of electrospun PCL/PMMA fibrous scaffolds for bone regeneration
Scaffolds were fabricated by electrospinning using polycaprolactone (PCL) blended with poly(methyl methacrylate) (PMMA) in ratios of 10/0, 7/3, 5/5 and 3/7. The PCL/PMMA ratio affected the fiber diameter, contact angle, tensile strength and biological in vitro and in vivo properties of the scaffolds...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2013-01-01
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| Series: | Science and Technology of Advanced Materials |
| Online Access: | http://dx.doi.org/10.1088/1468-6996/14/1/015009 |
| _version_ | 1811265207033397248 |
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| author | So-Ra Son, Nguyen-Thuy Ba Linh, Hun-Mo Yang and Byong-Taek Lee |
| author_facet | So-Ra Son, Nguyen-Thuy Ba Linh, Hun-Mo Yang and Byong-Taek Lee |
| author_sort | So-Ra Son, Nguyen-Thuy Ba Linh, Hun-Mo Yang and Byong-Taek Lee |
| collection | DOAJ |
| description | Scaffolds were fabricated by electrospinning using polycaprolactone (PCL) blended with poly(methyl methacrylate) (PMMA) in ratios of 10/0, 7/3, 5/5 and 3/7. The PCL/PMMA ratio affected the fiber diameter, contact angle, tensile strength and biological in vitro and in vivo properties of the scaffolds, and the 7/3 ratio resulted in a higher mechanical strength than 5/5 and 3/7. In vitro cytotoxicity and proliferation of MG-63 osteoblast cells on these blended scaffolds were examined by MTT assay, and it was found that PCL/PMMA blends are suitable for osteoblast cell proliferation. Confocal images and expression of proliferating cell nuclear antigen confirmed the good proliferation and expression of cells on the 7/3 PCL/PMMA fibrous scaffolds. In vivo bone formation was examined using rat models, and bone formation was observed on the 7/3 PCL/PMMA scaffold within 2 months. In vitro and in vivo results suggest that 7/3 PCL/PMMA scaffolds can be used for bone tissue regeneration. |
| first_indexed | 2024-04-12T20:18:21Z |
| format | Article |
| id | doaj.art-9deb23851b144156ad7e974b77534d5b |
| institution | Directory Open Access Journal |
| issn | 1468-6996 1878-5514 |
| language | English |
| last_indexed | 2024-04-12T20:18:21Z |
| publishDate | 2013-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Science and Technology of Advanced Materials |
| spelling | doaj.art-9deb23851b144156ad7e974b77534d5b2022-12-22T03:18:03ZengTaylor & Francis GroupScience and Technology of Advanced Materials1468-69961878-55142013-01-01141015009In vitro and in vivo evaluation of electrospun PCL/PMMA fibrous scaffolds for bone regenerationSo-Ra Son, Nguyen-Thuy Ba Linh, Hun-Mo Yang and Byong-Taek LeeScaffolds were fabricated by electrospinning using polycaprolactone (PCL) blended with poly(methyl methacrylate) (PMMA) in ratios of 10/0, 7/3, 5/5 and 3/7. The PCL/PMMA ratio affected the fiber diameter, contact angle, tensile strength and biological in vitro and in vivo properties of the scaffolds, and the 7/3 ratio resulted in a higher mechanical strength than 5/5 and 3/7. In vitro cytotoxicity and proliferation of MG-63 osteoblast cells on these blended scaffolds were examined by MTT assay, and it was found that PCL/PMMA blends are suitable for osteoblast cell proliferation. Confocal images and expression of proliferating cell nuclear antigen confirmed the good proliferation and expression of cells on the 7/3 PCL/PMMA fibrous scaffolds. In vivo bone formation was examined using rat models, and bone formation was observed on the 7/3 PCL/PMMA scaffold within 2 months. In vitro and in vivo results suggest that 7/3 PCL/PMMA scaffolds can be used for bone tissue regeneration.http://dx.doi.org/10.1088/1468-6996/14/1/015009 |
| spellingShingle | So-Ra Son, Nguyen-Thuy Ba Linh, Hun-Mo Yang and Byong-Taek Lee In vitro and in vivo evaluation of electrospun PCL/PMMA fibrous scaffolds for bone regeneration Science and Technology of Advanced Materials |
| title | In vitro and in vivo evaluation of electrospun PCL/PMMA fibrous scaffolds for bone regeneration |
| title_full | In vitro and in vivo evaluation of electrospun PCL/PMMA fibrous scaffolds for bone regeneration |
| title_fullStr | In vitro and in vivo evaluation of electrospun PCL/PMMA fibrous scaffolds for bone regeneration |
| title_full_unstemmed | In vitro and in vivo evaluation of electrospun PCL/PMMA fibrous scaffolds for bone regeneration |
| title_short | In vitro and in vivo evaluation of electrospun PCL/PMMA fibrous scaffolds for bone regeneration |
| title_sort | in vitro and in vivo evaluation of electrospun pcl pmma fibrous scaffolds for bone regeneration |
| url | http://dx.doi.org/10.1088/1468-6996/14/1/015009 |
| work_keys_str_mv | AT sorasonnguyenthuybalinhhunmoyangandbyongtaeklee invitroandinvivoevaluationofelectrospunpclpmmafibrousscaffoldsforboneregeneration |